Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   M3Y14_RS27360 Genome accession   NZ_CP097257
Coordinates   5182251..5183378 (+) Length   375 a.a.
NCBI ID   WP_002144552.1    Uniprot ID   J8DQA2
Organism   Bacillus thuringiensis strain Bt Gxmzu777-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5177251..5188378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3Y14_RS27330 (M3Y14_27240) spoIIIJ 5177287..5178054 (-) 768 WP_144506036.1 YidC family membrane integrase SpoIIIJ -
  M3Y14_RS27335 (M3Y14_27245) rnpA 5178107..5178454 (-) 348 WP_219916776.1 ribonuclease P protein component -
  M3Y14_RS27340 (M3Y14_27250) rpmH 5178531..5178665 (-) 135 WP_000831901.1 50S ribosomal protein L34 -
  M3Y14_RS27345 (M3Y14_27255) dnaA 5179229..5180569 (+) 1341 WP_219916777.1 chromosomal replication initiator protein DnaA -
  M3Y14_RS27350 (M3Y14_27260) dnaN 5180752..5181897 (+) 1146 WP_264539560.1 DNA polymerase III subunit beta -
  M3Y14_RS27355 (M3Y14_27265) yaaA 5182026..5182238 (+) 213 WP_002016484.1 S4 domain-containing protein YaaA -
  M3Y14_RS27360 (M3Y14_27270) recF 5182251..5183378 (+) 1128 WP_002144552.1 DNA replication/repair protein RecF Machinery gene
  M3Y14_RS27365 (M3Y14_27275) gyrB 5183417..5185339 (+) 1923 WP_219916779.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  M3Y14_RS27370 (M3Y14_27280) gyrA 5185428..5187911 (+) 2484 WP_264539561.1 DNA gyrase subunit A -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 43514.79 Da        Isoelectric Point: 6.8417

>NTDB_id=688186 M3Y14_RS27360 WP_002144552.1 5182251..5183378(+) (recF) [Bacillus thuringiensis strain Bt Gxmzu777-1]
MFITEIQLKNYRNYEHLELSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDRELIRWDEDYGNIKGRLQRRNSS
LSLELNISKKGKKAKLNQLEQQKLSQYIGEMNVVMFAPEDLNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQR
NHLLKKMQGNSKNEETMLDVFTLQLIEHGAKILRKRFEFLHLLQEWAAPIHRGISRGLEELEIIYKPSVDVSESMDLSKI
KEVYYESFQSVKQREIFRGTTLLGPHRDDLQFFVNSKNVQVFGSQGQQRTTALSLKLAEIELIYSEVKEYPILLLDDVLS
ELDDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKEAKTIHVTNGTVDCEIDRK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=688186 M3Y14_RS27360 WP_002144552.1 5182251..5183378(+) (recF) [Bacillus thuringiensis strain Bt Gxmzu777-1]
TTGTTTATTACAGAAATACAATTAAAAAACTATCGCAATTATGAGCATTTAGAGCTTTCCTTTGAGGATAAAGTCAATGT
AATTATTGGTGAAAATGCGCAAGGGAAAACGAATCTAATGGAAGCTATTTATGTATTGGCGATGGCGAAATCCCATAGAA
CTTCGAACGATCGTGAACTCATTCGCTGGGATGAGGATTATGGTAATATAAAAGGTAGATTACAAAGGCGAAATAGTTCT
TTATCCTTAGAATTAAATATTTCCAAAAAAGGTAAAAAGGCAAAGTTAAATCAATTAGAACAGCAAAAATTGAGCCAGTA
TATTGGTGAAATGAATGTTGTTATGTTTGCCCCAGAAGATTTAAATCTTGTAAAAGGAAGCCCTCAAGTACGAAGACGCT
TTTTAGATATGGAACTTGGACAAATAGCTCCGGTCTATTTGTATGAATTAAGCCAATATCAAAAGGTACTCACGCAACGA
AATCATTTACTTAAGAAGATGCAAGGGAATAGTAAAAATGAGGAAACGATGCTGGATGTGTTTACACTCCAACTTATTGA
GCATGGTGCAAAAATTTTGCGAAAACGTTTTGAGTTTTTACATTTACTACAAGAATGGGCTGCTCCAATTCACCGCGGAA
TTAGCAGGGGATTAGAGGAGTTAGAAATCATCTATAAACCAAGTGTAGATGTATCAGAATCAATGGATTTGTCGAAAATA
AAAGAAGTATACTATGAAAGTTTTCAATCTGTGAAACAACGTGAAATTTTTCGTGGTACAACTTTACTCGGTCCTCATCG
TGATGATTTACAATTCTTCGTTAATAGTAAAAATGTTCAAGTCTTTGGTTCACAAGGACAACAACGAACAACTGCACTAT
CCCTAAAATTGGCTGAAATTGAATTGATATACTCAGAGGTTAAGGAATATCCAATCCTTTTATTAGATGATGTGTTATCA
GAACTAGATGATTATCGTCAATCACATCTTTTAAATACGATTCAAGGAAAAGTACAAACATTTGTGACCACGACGAGTGT
CGACGGAATTGAACACGAAACATTGAAAGAAGCGAAAACAATTCATGTAACGAACGGCACGGTAGATTGTGAAATAGACA
GAAAATAA

Domains

Predicted by InterProScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

67.12

98.133

0.659