Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   M3Y14_RS21575 Genome accession   NZ_CP097257
Coordinates   4081968..4082714 (-) Length   248 a.a.
NCBI ID   WP_002129136.1    Uniprot ID   A0A366GTN2
Organism   Bacillus thuringiensis strain Bt Gxmzu777-1     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4076968..4087714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3Y14_RS21555 (M3Y14_21480) dnaG 4077700..4079502 (-) 1803 WP_264539169.1 DNA primase -
  M3Y14_RS21560 (M3Y14_21485) - 4079804..4079995 (-) 192 WP_264539170.1 hypothetical protein -
  M3Y14_RS21565 (M3Y14_21490) - 4080387..4081199 (-) 813 WP_070144890.1 pyruvate, water dikinase regulatory protein -
  M3Y14_RS21570 (M3Y14_21495) - 4081229..4081861 (-) 633 WP_105584761.1 helix-turn-helix transcriptional regulator -
  M3Y14_RS21575 (M3Y14_21500) recO 4081968..4082714 (-) 747 WP_002129136.1 DNA repair protein RecO Machinery gene
  M3Y14_RS21580 (M3Y14_21505) - 4082748..4082891 (-) 144 WP_000883924.1 YqzL family protein -
  M3Y14_RS21585 (M3Y14_21510) era 4083068..4083973 (-) 906 WP_002122584.1 GTPase Era -
  M3Y14_RS21590 (M3Y14_21515) - 4083966..4084364 (-) 399 WP_002111886.1 cytidine deaminase -
  M3Y14_RS21600 (M3Y14_21525) - 4084464..4084872 (-) 409 Protein_4287 diacylglycerol kinase family protein -
  M3Y14_RS21605 (M3Y14_21530) ybeY 4084869..4085339 (-) 471 WP_002199328.1 rRNA maturation RNase YbeY -
  M3Y14_RS21610 (M3Y14_21535) - 4085336..4087480 (-) 2145 WP_002199327.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28668.23 Da        Isoelectric Point: 8.0540

>NTDB_id=688169 M3Y14_RS21575 WP_002129136.1 4081968..4082714(-) (recO) [Bacillus thuringiensis strain Bt Gxmzu777-1]
MFQKVEGIVIRTTDYGETNKIVTIYSREFGKVSAMARGAKKPKSRLAAISQLMTHGHFLIQMGSGLGTLQQGEIISSMKE
IREDIFLTAYASFIIELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDPYRIPVGEAVHKLLRLFFHFDLHRLGNVSVKDSTKKQMRTVLNTYYDEYCGIYLKTRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=688169 M3Y14_RS21575 WP_002129136.1 4081968..4082714(-) (recO) [Bacillus thuringiensis strain Bt Gxmzu777-1]
ATGTTTCAAAAAGTTGAGGGCATTGTTATCCGTACGACAGATTACGGAGAAACAAATAAGATTGTTACAATATACTCACG
GGAATTTGGTAAGGTAAGTGCAATGGCAAGAGGGGCGAAAAAGCCGAAGAGTCGGTTAGCGGCTATTTCGCAACTTATGA
CACATGGTCATTTTCTTATTCAAATGGGATCTGGACTTGGAACTTTGCAACAAGGCGAGATTATTTCATCTATGAAAGAA
ATTCGTGAGGATATATTTTTAACTGCTTATGCATCATTTATTATTGAACTAACCGATAAAGCAACAGAAGATAAGAAGCA
TAATCCGTATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTACCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGATACATGTGCTATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTCCGGGAAGGCGGTTTTCTGTGCTCGCGTCATGCGGAGCAAGATCCGTATCGTATTCCGGTGGG
AGAGGCTGTTCATAAATTATTACGTCTCTTTTTTCACTTTGATTTACACCGGCTCGGTAATGTATCGGTGAAGGATAGTA
CAAAAAAACAAATGCGCACAGTATTGAATACATATTATGATGAATATTGTGGGATTTATTTGAAAACAAGGCGTTTCTTG
GAGCAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A366GTN2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552