Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   M3Y14_RS21305 Genome accession   NZ_CP097257
Coordinates   4028776..4029819 (-) Length   347 a.a.
NCBI ID   WP_264539152.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain Bt Gxmzu777-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4023776..4034819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3Y14_RS21260 (M3Y14_21185) - 4024145..4024345 (-) 201 WP_264539149.1 YqzE family protein -
  M3Y14_RS21265 (M3Y14_21190) - 4024384..4024881 (-) 498 WP_264539150.1 shikimate kinase -
  M3Y14_RS21270 (M3Y14_21195) - 4025064..4025714 (-) 651 WP_219920180.1 2OG-Fe(II) oxygenase -
  M3Y14_RS21275 (M3Y14_21200) comGG 4025887..4026258 (-) 372 WP_219920182.1 competence type IV pilus minor pilin ComGG -
  M3Y14_RS21280 (M3Y14_21205) comGF 4026255..4026716 (-) 462 WP_219920184.1 competence type IV pilus minor pilin ComGF -
  M3Y14_RS21285 (M3Y14_21210) - 4026700..4026996 (-) 297 WP_219921083.1 type II secretion system protein -
  M3Y14_RS21290 (M3Y14_21215) comGD 4026989..4027444 (-) 456 WP_219920187.1 competence type IV pilus minor pilin ComGD -
  M3Y14_RS21295 (M3Y14_21220) comGC 4027441..4027740 (-) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  M3Y14_RS21300 (M3Y14_21225) comGB 4027752..4028789 (-) 1038 WP_319802182.1 competence type IV pilus assembly protein ComGB -
  M3Y14_RS21305 (M3Y14_21230) comGA 4028776..4029819 (-) 1044 WP_264539152.1 competence type IV pilus ATPase ComGA Machinery gene
  M3Y14_RS21310 (M3Y14_21235) - 4030025..4030720 (+) 696 WP_219920193.1 helix-turn-helix transcriptional regulator -
  M3Y14_RS21315 (M3Y14_21240) - 4030850..4031092 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  M3Y14_RS21320 (M3Y14_21245) - 4031200..4032594 (+) 1395 WP_264539153.1 L-cystine transporter -
  M3Y14_RS21325 (M3Y14_21250) - 4032838..4033237 (-) 400 Protein_4234 hypothetical protein -
  M3Y14_RS21330 (M3Y14_21255) - 4033325..4033540 (-) 216 WP_219920197.1 DUF3912 family protein -
  M3Y14_RS21335 (M3Y14_21260) - 4033793..4034104 (+) 312 WP_098777139.1 hypothetical protein -
  M3Y14_RS21340 (M3Y14_21265) - 4034311..4034799 (-) 489 WP_219920201.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39049.33 Da        Isoelectric Point: 8.6528

>NTDB_id=688168 M3Y14_RS21305 WP_264539152.1 4028776..4029819(-) (comGA) [Bacillus thuringiensis strain Bt Gxmzu777-1]
MNGIELFANTIMKEACGVQASDLHIVPRQKDVAIQLRIGKDLITKWCIEKEFGKKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKGQTRRIVTLEDPVERRKDGLLQIQINEKAGITYETGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQAIKEASGEH
VTYHYETLEASVRKGYALGFLEEDIYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=688168 M3Y14_RS21305 WP_264539152.1 4028776..4029819(-) (comGA) [Bacillus thuringiensis strain Bt Gxmzu777-1]
ATGAATGGTATCGAGCTTTTTGCGAATACGATTATGAAAGAAGCTTGTGGGGTGCAAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCAATTCAATTACGTATAGGAAAAGATTTAATTACGAAATGGTGTATTGAAAAGGAATTTGGAA
AAAAGCTTGTTTCGCACTTTAAGTTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGTTCAGCCATTGTCTCATCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGATTACTCGTATTTACTGGGCCGACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAAGGGCAAACACGTCGCATCGTTACACTTGAAGATCCAGTTGAGAGAAGAAAGGACGGTTTATTACAAATTCA
AATTAATGAAAAAGCAGGTATCACATATGAGACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATATTAGTTG
GTGAAATCCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGAGCAAGTTTGACAGGCCATTTAGTAATGACGACGTTGCAT
ACAAATGACGCGAAAGGGGCGATATTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTGGC
TGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCGTTTTGCAGAGGGAAGTGTTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAGGCGAGTATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGCGGAGAACAT
GTTACGTATCACTATGAAACATTGGAAGCATCAGTGCGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATATTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.637

100

0.576
  pilB Glaesserella parasuis strain SC1401

43.86

82.133

0.36