Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   M3Y14_RS20090 Genome accession   NZ_CP097257
Coordinates   3824603..3825037 (-) Length   144 a.a.
NCBI ID   WP_219919869.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain Bt Gxmzu777-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3819603..3830037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3Y14_RS20075 (M3Y14_20005) - 3820745..3821302 (+) 558 WP_264539085.1 PadR family transcriptional regulator -
  M3Y14_RS20080 (M3Y14_20010) metE 3821844..3824132 (+) 2289 WP_264539086.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  M3Y14_RS20085 (M3Y14_20015) comJ 3824174..3824563 (-) 390 WP_219919867.1 competence protein ComJ -
  M3Y14_RS20090 (M3Y14_20020) nucA/comI 3824603..3825037 (-) 435 WP_219919869.1 DNA-entry nuclease Machinery gene
  M3Y14_RS20095 (M3Y14_20025) - 3825274..3826017 (+) 744 WP_264539087.1 DUF3967 domain-containing protein -
  M3Y14_RS20100 (M3Y14_20030) - 3826077..3827351 (-) 1275 WP_219919876.1 sensor histidine kinase -
  M3Y14_RS20105 (M3Y14_20035) - 3827661..3827861 (+) 201 WP_219919878.1 hypothetical protein -
  M3Y14_RS20110 (M3Y14_20040) - 3828103..3829266 (+) 1164 WP_264539088.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16180.56 Da        Isoelectric Point: 8.4331

>NTDB_id=688165 M3Y14_RS20090 WP_219919869.1 3824603..3825037(-) (nucA/comI) [Bacillus thuringiensis strain Bt Gxmzu777-1]
MKQFKGIIISIIAILSLLVAVYEVLVPEETSTKKTTTYDQILEFPKERYPETGKHITDAIKDGHSEMCTIDRNGAADRRK
LSLAPYPTKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=688165 M3Y14_RS20090 WP_219919869.1 3824603..3825037(-) (nucA/comI) [Bacillus thuringiensis strain Bt Gxmzu777-1]
ATGAAGCAATTTAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTCTTTTGGTAGCGGTATATGAAGTACTTGTGCC
AGAGGAGACAAGTACTAAAAAAACAACTACGTATGATCAAATTCTAGAGTTCCCGAAAGAACGATATCCAGAGACCGGGA
AACATATAACAGATGCTATAAAAGATGGGCATTCAGAAATGTGTACAATTGATCGTAATGGTGCAGCGGATAGAAGGAAA
TTATCGTTAGCGCCATATCCGACGAAGAAAGGCTATGATCGTGATGAATGGCCGATGGCGATGTGTAAGGAAGGCGGAAA
AGGAGCTCATATTGAATATATAAGTCCAGCAGATAACCGCGGTGCAGGTTCTTGGGTAGGGAATAAGCTAGACAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAATAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(47-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

68.142

78.472

0.535