Detailed information NTG0688144

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: M3Y14_RS05520
• Genome accession: NZ_CP097257
• Coordinates: 1076257..1079982 (+)
• Length: 1241 a.a.
• NCBI ID: WP_264540330.1
• Uniprot ID: -
• Organism: Bacillus thuringiensis strain Bt Gxmzu777-1
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1071257..1084982

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M3Y14_RS05505 (M3Y14_05480)	-	1071415..1072008 (+)	594	WP_078179996.1	TVP38/TMEM64 family protein	-
M3Y14_RS05510 (M3Y14_05485)	lepB	1072065..1072628 (+)	564	WP_002149908.1	signal peptidase I	-
M3Y14_RS05515 (M3Y14_05490)	addB	1072745..1076260 (+)	3516	WP_219917448.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
M3Y14_RS05520 (M3Y14_05495)	addA	1076257..1079982 (+)	3726	WP_264540330.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
M3Y14_RS05525 (M3Y14_05500)	-	1080006..1080284 (+)	279	WP_219917450.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
M3Y14_RS05530 (M3Y14_05505)	gerPF	1080410..1080625 (-)	216	WP_098385040.1	spore germination protein GerPF	-
M3Y14_RS05535 (M3Y14_05510)	-	1080668..1081054 (-)	387	WP_219917451.1	spore germination protein GerPE	-
M3Y14_RS05540 (M3Y14_05515)	gerPD	1081069..1081263 (-)	195	WP_002149893.1	spore germination protein GerPD	-
M3Y14_RS05545 (M3Y14_05520)	-	1081270..1081884 (-)	615	WP_002087034.1	spore germination protein GerPC	-
M3Y14_RS05550 (M3Y14_05525)	gerPB	1081949..1082155 (-)	207	WP_002149891.1	spore germination protein GerPB	-
M3Y14_RS05555 (M3Y14_05530)	gerPA	1082170..1082391 (-)	222	WP_002149890.1	spore germination protein GerPA	-
M3Y14_RS05560 (M3Y14_05535)	-	1082488..1082667 (-)	180	WP_002149889.1	aspartyl-phosphate phosphatase Spo0E family protein	-
M3Y14_RS05565 (M3Y14_05540)	-	1083084..1083476 (+)	393	WP_264540331.1	zinc ribbon domain-containing protein	-
M3Y14_RS05570 (M3Y14_05545)	-	1083649..1084548 (+)	900	WP_219917453.1	fumarylacetoacetate hydrolase family protein	-
M3Y14_RS05575 (M3Y14_05550)	-	1084584..1084868 (-)	285	WP_219917454.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142628.02 Da
• Isoelectric Point: 4.9171

>NTDB_id=688144 M3Y14_RS05520 WP_264540330.1 1076257..1079982(+) (addA) [Bacillus thuringiensis strain Bt Gxmzu777-1]
MIENWPQKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIISEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDGPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEEHI
RKATELAMLPDGPAPRVETLQADLALLGMLSSAARGSWTGVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVKLVKVFTERFQAIKRDKGMVDFTDLEHFCLQILSEQSEAGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLDGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGNIPSDIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKDEVYDRLMWKYGYEDATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIARMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLSAEEAYQDWQGKKGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLKHPVKEKCLYFFDGNHVVKVEE

Nucleotide

• Length: 3726 bp

>NTDB_id=688144 M3Y14_RS05520 WP_264540330.1 1076257..1079982(+) (addA) [Bacillus thuringiensis strain Bt Gxmzu777-1]
ATGATAGAAAATTGGCCTCAGAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCAGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAGTG
AGGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAAATGAAAAATCGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGGGCCAGGTTCACAGCATATAAGAAAACAGCTTAGCTTATTAAATAAAGC
TTCCATTTCTACCATTCATTCATTTTGTTTACAAGTTATTAGAGGATATTATTACATGCTTGATGTCGATCCTCGTTTTC
GTATTGCGAACCAAACAGAAAATGAGTTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATCGAA
GATAATAGTATTTTCTTTGAATTAGTTGATCGTTATACGAGTGACCGTAGTGACGATGACTTACAACGAATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCGAACCCGGAGAAGTGGCTCGATAAATTAGTAGAAGCATACGATGTTGAAGGCA
AGACGATTGAAGATTTAGTGTACGCTTCTTACTTATTAGAAGATGTGAAATTCCAGCTGGAAACAGCGGAAGAGCATATT
CGTAAAGCGACTGAACTCGCAATGCTTCCTGATGGTCCGGCGCCTCGCGTTGAAACCCTGCAAGCGGATTTAGCTTTACT
TGGAATGTTATCCTCAGCAGCTCGTGGATCGTGGACAGGCGTTTATGAAGCGATGCAAAATGTATCGTGGCAAACGCTAA
AGCGTATTAAGAAAAGTGATTATAACGAAGATGTTGTAAAACAAGTAGATTCTCTTCGTAATAAAGCGAAAGATGAAGTA
AAGAAATTACAAGAAGAGCTATTTAGCCGCAGACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTGAAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCAATTAAACGAGATAAAGGAATGGTTGATTTCACAG
ATTTAGAGCATTTCTGTTTGCAAATTTTAAGTGAGCAAAGTGAAGCCGGTGAAATGAAGCCGTCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTTGATGAATATCAAGATACGAACTTCGTACAGGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAACAGTCAATCTATCGTTTCCGACTAGCAG
AGCCTGGTTTATTCTTAGGAAAATATAAACGTTTCACACAAGAAGGATTGGACGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGCCGTCATGAAGTGCTAGCAGGTACGAACTTTATTTTCAAACAAATTATGGGCGAAGAAGTTGGAGAGAT
TGACTACGACGCTGACGCTGAATTAAAACTAGGCGCTAGTTATCCAGAAGGTGAAGATGTAGCGGCTGAATTATTATGTA
TTCAGCAAACGGAAGAAGAAGTAATAGATGGTGAAGAAGGTGCAGAAGTAGAAAAAGCGCAGCTTGAAGCTCGTCTTATG
GCACAGCGCATTAAAGCGATGGTCGATTCAGGTTATGAAGTGTATGACCGAAAAACGGATAGTATGCGACCAGTACAATA
CCGTGATTTCGTTATTTTACTTCGCTCTATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAACTACAAGGAATTCCAG
TATATGCAGACCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCACTTGCAGCAGTACTTCGTTCACCGATCGTTGGACTAAATGACGAAGAACTTGCGACGCTTCG
TGCTCATGGAAAGAAAGGGTCATTTTATGAAGTAATGAGTTCATTCTTAAAAGGAGCACCGCTTGAAGAAGAACAAGAAC
TGCATGATAAATTAGAGTGGTTTTATAACTTACTGCAAGGATGGCGTGAATTTGCGCGCCAACAATCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGGTATTATGACTTTGTCGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
GCGCGTATTATATGACCGCGCAAGGCAATATGAAGCAACTTCATTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGACGATATGGGTACGGCGAGAGCTCTCGGTGAACAAGAAGATGTTGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCGGTCGTATTTGTAGCTGGACTTGGTCGCCGTTTTAATACACAAGATTTAATGAA
GCGTTTCTTACTTCATAAAGATTTCGGTTTCGGTTCACAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCGATTAAGCGTAAAATGAAGATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCGCTAACGCGT
GCAAAAGAGAAGTTAATTTTAATTGGAACGGTTAAAGATGCAAATAAAGAAATGGAAAAATGGCTCGATGCAAGAGAGCA
TAGTGAATGGTTATTACCAGACCATATACGTGCCGGAGCGTCATGTTATTTAGATTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAATATTCCAAGTGACATTTATGGATATGACACTAGCTGGAAA
GTAGAAGTTGTTGACGGCAACACGTTACTCGCACCAGAACCGGTTCAAGAAGAGAAACAAGAATTACTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCGCTACAAAGTGAACGAAAAGATGAAGTATACGACAGATTAATGTGGAAGTACGGATATGAGG
ACGCGACATCTCACCGTGCGAAACAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGCAGCGATAATGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGGCTAACATACGCAGAGCGAGG
GACAGCAGTCCATGCCGTTATGCAGCATGTAGATTTGAAGAAACCAATTACGATTGAAGTTCTTCAAGAACAAATTGCAA
GAATGGTAAATAAAGAACTATTAACATTTGAACAAGCTGAAGAAATAGCGATTGAAAAGGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGAGTATTAGCAGCAAAAAGTGTTGAGCGCGAAGTACCATTTACGATGATGCTTTCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGGAAGAAAGGCGAATCAATACTTGTCCAAGGGGTTATCGACTGCATGATCGAAGAGGAAGACG
GAATTACTTTAATCGACTTTAAAACAGATACGATTGAAGGAAAGTTTCCAGGCGGATTTGATCAAGCGAAACCAATTTTA
GAAGACCGATACAAAGTACAGCTTTCACTATATGCAAAAGCACTCGAGAAAAGCTTAAAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGGAATCATGTTGTAAAGGTTGAGGAATAG

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1232)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.488	100	0.537