Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   M3Y14_RS05515 Genome accession   NZ_CP097257
Coordinates   1072745..1076260 (+) Length   1171 a.a.
NCBI ID   WP_219917448.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain Bt Gxmzu777-1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1067745..1081260
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3Y14_RS05485 (M3Y14_05460) cspA 1068102..1068305 (+) 204 WP_000218989.1 RNA chaperone/antiterminator CspA -
  M3Y14_RS35110 - 1068366..1068591 (+) 226 Protein_1062 hypothetical protein -
  M3Y14_RS05490 (M3Y14_05465) - 1068641..1068826 (-) 186 WP_219917445.1 hypothetical protein -
  M3Y14_RS05495 (M3Y14_05470) - 1069636..1070217 (+) 582 WP_219917446.1 competence protein ComK -
  M3Y14_RS05500 (M3Y14_05475) - 1070462..1071058 (+) 597 WP_219917447.1 DUF4306 domain-containing protein -
  M3Y14_RS05505 (M3Y14_05480) - 1071415..1072008 (+) 594 WP_078179996.1 TVP38/TMEM64 family protein -
  M3Y14_RS05510 (M3Y14_05485) lepB 1072065..1072628 (+) 564 WP_002149908.1 signal peptidase I -
  M3Y14_RS05515 (M3Y14_05490) addB 1072745..1076260 (+) 3516 WP_219917448.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  M3Y14_RS05520 (M3Y14_05495) addA 1076257..1079982 (+) 3726 WP_264540330.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  M3Y14_RS05525 (M3Y14_05500) - 1080006..1080284 (+) 279 WP_219917450.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  M3Y14_RS05530 (M3Y14_05505) gerPF 1080410..1080625 (-) 216 WP_098385040.1 spore germination protein GerPF -
  M3Y14_RS05535 (M3Y14_05510) - 1080668..1081054 (-) 387 WP_219917451.1 spore germination protein GerPE -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134238.38 Da        Isoelectric Point: 5.1796

>NTDB_id=688143 M3Y14_RS05515 WP_219917448.1 1072745..1076260(+) (addB) [Bacillus thuringiensis strain Bt Gxmzu777-1]
MSLRLVIGRAGSGKSALCLREVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLTQSEYVNGAEIYIDGFHSFSPQELEILRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEVEKTIPLMEQPRFHSQALAHLEMHYEARPNEKFHGEASVTISTAANL
RAEVEGVAREIRRLVADEKYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEAIREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDASEMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMTTGLEALQFANIPPSLDQVLVANIDHSRLSDVKATFIIGVNEGVIPAAPMDEGMLSDEERNVLTAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKESFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTDVSRDLYGDTIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADKLLRENRTWADLSIKECEHLSTLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSLILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFALPNGVKMEVVGRIDRVDKAEDESG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGSTASPAGVLYFHIHNPIVEMKGDASEEEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHSFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=688143 M3Y14_RS05515 WP_219917448.1 1072745..1076260(+) (addB) [Bacillus thuringiensis strain Bt Gxmzu777-1]
ATGTCACTTCGATTGGTGATTGGTAGAGCGGGAAGTGGAAAGAGTGCACTTTGTTTACGAGAAGTGCAAGAAGAGTTAAA
ACAGCGCCCGAGAGGCAAAACAATATTATATCTTGTGCCAGAACAGATGACATTTCAAACGCAGCAGGCGTTAATTGGAA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTATTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCGGGTGTGCATATGTTACTTCGTAAAATTGTAGAATCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGAAGTATGATTGCGGAGTTTAAACGTTACA
ATGTGACGCCGTCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCAGAGCAAAAGCTACTAGCG
AATAAAGTATATGATTTACAGCTATTATATGATGATTTTGAACGTGCTTTAATCGGGAAATATTTAGATTCAGAAGACTA
TTTACAATTGCTAGTGGAAAAGCTCACGCAGTCTGAGTATGTAAATGGTGCTGAAATTTATATAGATGGGTTTCATTCAT
TTTCTCCGCAAGAACTTGAAATTTTAAGACAGCTCATGATTTGTGGAGCGAGAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTGAATGAACTGGATTTATTTTATGAAACGACGTTAACATATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAGTTGAAAAAACAATTCCACTTATGGAACAGCCGCGTTTTCATTCGCAAGCATTAGCGCATT
TAGAAATGCATTACGAAGCGCGTCCAAATGAAAAGTTTCACGGTGAAGCGAGTGTGACAATTAGTACAGCAGCTAATTTA
CGAGCTGAAGTAGAAGGTGTTGCTCGTGAAATTCGTAGACTTGTCGCGGACGAAAAGTATCGTTACCGAGATATTGCAGT
ACTTCTTCGTAACGGGGAAAGTTATTACGATGTGATGCGAACGTTATTTACAGATTATAATATTCCGCACTTCATCGATG
AAAAACGCCCGATGTCACATCATCCGCTAGTAGAATGTATTCGTTCGGCTTTAGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTGTTCCGCTGTGTGAAAACAGAACTTTTATATCCACTAGACGTAAGAAAAGAAGCGATCCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAAGATCCGTGGATGTATCGCCGTT
ATCGTTCTCTTGATGATGCGAGCGAAATGATAACAGACAGTGAGCGTGAAATGGAAGAGAAAATAAATCGACTTCGCGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGACTGAAGCGTGCTGGAACAGTTATGCAAATGTGCGAAGCGGTATA
TTTATTTTTAGAGGAGCTTGACGTTCCGAAAAAACTAGAAGCATTACGTATTCGTGCAGAAGAAAGCGGAGATTTCTTAT
TTGCGACAGACCATGAGCAAGTATGGGAAGAAGTGATGAGCCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAG
ATGTCCCTGTCTATGTTTACTGATGTAATGACGACGGGTCTTGAGGCACTTCAATTTGCGAACATTCCGCCGTCATTAGA
TCAAGTATTAGTTGCTAATATTGATCATTCTAGATTATCAGATGTTAAAGCGACGTTCATTATTGGAGTAAATGAAGGAG
TAATTCCAGCAGCGCCTATGGATGAAGGTATGCTTTCTGATGAGGAAAGAAATGTTCTCACCGCTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTATCAAATGGTGACGAGAGCATCTGAGAAGTTATA
CATTTCTTGTCCGCTTGCGGATGAAGAAGGAAAGACGTTACTTGCTTCTAGTTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGAATCGTTTATTACGAATGATGTAAATGACTTATCGCGTTCGGAACAAATCTCATATGTAGCAACGCCG
GAAGTAACTTTGTCTTACGTCATGCAGCAACTGCAAACGTGGAAGCGCTACGGATTTGAAGGCAATTTAGATTTTTGGTG
GGACGTATATAACTTCTACGTTACTTCAGATGAATGGAAGCAAAAAAGTAGCCGCGTGTTATCGAGTTTATTCTACCGAA
ATCGTGCACAGAAACTAAGTACAGATGTAAGTAGGGATTTATATGGAGATACAATTAAGGGAAGCGTTTCTCGTATGGAA
CTATTTAATCGCTGTGCGTACGCTCATTTCGCTCAGCACGGTTTATCGCTAAGAGAGCGTGATATTTTCAAACTTGATGC
GCCAGATATCGGTGAATTATTCCATGCAGCGCTGAAGAGAATTGCAGACAAGCTATTACGTGAAAATCGTACTTGGGCAG
ATTTATCAATAAAAGAGTGTGAGCACCTTTCTACTTTAGTAATAGAAGAAATTGCACCATTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACATCACTTATTCTTCGTGAACA
TGCGAAGTCGAGTGGCTTTGTACCAGTTGATTTAGAAGTACCGTTCGGTATGGGCGGGACTGGATCGCTTCCGCCAATGG
AATTTGCGTTGCCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCGGAAGATGAGAGTGGT
ACGTTCCTTCGTATTATCGACTATAAATCAAGTTCGAAAGCGTTAGATTTAACAGAAGTGTATTACGGACTCGCACTTCA
AATGCTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAGGGCAGTACTGCATCACCGGCGGGTG
TATTGTATTTCCATATTCATAACCCAATTGTTGAGATGAAAGGCGATGCATCTGAAGAAGAAATTGAGAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGGCTCGTACTAGGAGATGCTGACGTTGTTCGTTTAATGGATAATAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGATGGTAGTTTTAGTGCGCGTTCTAGCATTGCAAGTGAACAAGAGTTTA
ACGTACTTCAAAAATATGTACACCACTCGTTTGAAAATATCGGAAAAGACATTACAGAAGGTGTTATCGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCATGTACGTTCTGTAACTTTAAATCTGTTTGTCAGTTTGATGAATCACTTGAAGATAA
CCAATTCCGTACGTTAAAAGATATGAAAGATAGCGAAGCAATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

48.978

100

0.491