Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M3Y14_RS00390 Genome accession   NZ_CP097257
Coordinates   69963..71339 (+) Length   458 a.a.
NCBI ID   WP_071747351.1    Uniprot ID   A0AA44KSC4
Organism   Bacillus thuringiensis strain Bt Gxmzu777-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 64963..76339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3Y14_RS00370 (M3Y14_00365) ctsR 65162..65623 (+) 462 WP_002115277.1 transcriptional regulator CtsR -
  M3Y14_RS00375 (M3Y14_00370) - 65793..66341 (+) 549 WP_219916810.1 UvrB/UvrC motif-containing protein -
  M3Y14_RS00380 (M3Y14_00375) - 66346..67410 (+) 1065 WP_264539920.1 protein arginine kinase -
  M3Y14_RS00385 (M3Y14_00380) clpC 67433..69868 (+) 2436 WP_219916812.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  M3Y14_RS00390 (M3Y14_00385) radA 69963..71339 (+) 1377 WP_071747351.1 DNA repair protein RadA Machinery gene
  M3Y14_RS00395 (M3Y14_00390) disA 71343..72416 (+) 1074 WP_070140523.1 DNA integrity scanning diadenylate cyclase DisA -
  M3Y14_RS00400 (M3Y14_00395) - 72578..73681 (+) 1104 WP_199640145.1 PIN/TRAM domain-containing protein -
  M3Y14_RS00405 (M3Y14_00400) ispD 73698..74378 (+) 681 WP_219916813.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  M3Y14_RS00410 (M3Y14_00405) ispF 74492..74968 (+) 477 WP_098366211.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49806.47 Da        Isoelectric Point: 7.0904

>NTDB_id=688136 M3Y14_RS00390 WP_071747351.1 69963..71339(+) (radA) [Bacillus thuringiensis strain Bt Gxmzu777-1]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLDVKGSNLFVVAETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKATAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=688136 M3Y14_RS00390 WP_071747351.1 69963..71339(+) (radA) [Bacillus thuringiensis strain Bt Gxmzu777-1]
ATGGCTAAAAAGAAAACGAAATTTACATGTCAAGAATGTGGTTATCAGTCGCCAAAATATATGGGGAAGTGTCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCAGTTGTATCATCAAGACGCCTTAATTATGCCAATGCAATTCAAT
CAGAAGTAACAAAACCAAGACGCTTAACAGAGGTGGAAACAAAGTCTGAGGCGCGTATTGAAACGAAATTTCAAGAGTTT
AACCGTGTGCTTGGTGGCGGGATTGTAGATGGATCTTTAGTACTTATTGGTGGAGACCCTGGAATTGGGAAATCAACATT
GTTATTACAAATTTCATCGCAATTAGCAGATTCTTCATATGATGTACTATACATATCAGGTGAGGAGTCAGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTGGATGTAAAGGGTAGTAATCTATTTGTTGTAGCAGAGACTGATTTACAGCGAATTGCA
GCGCACATTGAAGAGATGAATCCAGCTTTTGTTGTTATTGACTCTATTCAGACGATACATTTACCTGAAGTGACGTCAGC
CCCAGGGAGTGTAGCGCAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAGACGAAAGGGATTCCTATTTTTA
TCGTCGGACATGTGACAAAAGAAGGGGCAATTGCAGGACCACGTATGCTAGAACATATGGTTGATGCAGTTCTTTACTTT
GAAGGAGATCGTCACCATACATATCGTATTTTGCGAGCTGTGAAGAACCGTTTTGGTTCCACAAATGAAATGGGTATCTT
TGAAATGAAGGAACTAGGTCTTGCAGAAGTACTAAATCCTTCTGAAATTTTCCTTGAGGAACGACCAGTTGGAGTTGCAG
GATCCACAGTGGTTGCTTCAATGGAAGGAACAAGACCGGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGGAATCCTCGAAGAATGGCGACGGGAATTGATCATAACCGCGTTTCACTTATTATGGCAGTGCTAGAAAAAAGAACAGG
TTTATTGTTGCAAAATCAAGACGCATATTTAAAAGTTGCGGGTGGCTTGAAATTAGATGAACCAGCAATCGATTTAGCTG
TCGCCTTAAGTATTGCTTCAAGTTTCAGAGATAAAGCTACTGCACCAACCGATGCGGTAATAGGAGAAGTAGGCCTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCGAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCAGATGGGATTGAGGTAGTAGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.51

100

0.747
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631