Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M3M92_RS06050 Genome accession   NZ_CP097165
Coordinates   1237707..1239080 (-) Length   457 a.a.
NCBI ID   WP_003702635.1    Uniprot ID   F5VC94
Organism   Ligilactobacillus salivarius strain VHProbi A17     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1232707..1244080
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3M92_RS06030 (M3M92_06005) - 1232985..1233392 (-) 408 WP_003700685.1 Mini-ribonuclease 3 -
  M3M92_RS06035 (M3M92_06010) cysS 1233385..1234797 (-) 1413 WP_069469113.1 cysteine--tRNA ligase -
  M3M92_RS06040 (M3M92_06015) gltX 1234951..1236438 (-) 1488 WP_003700687.1 glutamate--tRNA ligase -
  M3M92_RS06045 (M3M92_06020) - 1236569..1237681 (-) 1113 WP_003706511.1 PIN/TRAM domain-containing protein -
  M3M92_RS06050 (M3M92_06025) radA 1237707..1239080 (-) 1374 WP_003702635.1 DNA repair protein RadA Machinery gene
  M3M92_RS06055 (M3M92_06030) - 1239098..1239634 (-) 537 WP_069469112.1 hypothetical protein -
  M3M92_RS06060 (M3M92_06035) - 1239790..1240068 (+) 279 WP_003702618.1 GNAT family N-acetyltransferase -
  M3M92_RS06065 (M3M92_06040) - 1240157..1241491 (+) 1335 WP_069469111.1 C1 family peptidase -
  M3M92_RS06070 (M3M92_06045) - 1241701..1242054 (+) 354 WP_069469110.1 bacteriocin immunity protein -
  M3M92_RS06075 (M3M92_06050) - 1242224..1242898 (-) 675 WP_003706506.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  M3M92_RS06080 (M3M92_06055) - 1242920..1243864 (-) 945 WP_003700695.1 AEC family transporter -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49722.37 Da        Isoelectric Point: 8.0254

>NTDB_id=687577 M3M92_RS06050 WP_003702635.1 1237707..1239080(-) (radA) [Ligilactobacillus salivarius strain VHProbi A17]
MAKTKTRYVCQNCEYISPRYLGRCPNCGSWNSLVEEIEKKESSTKAQPRVSISGTTVKPKLIDDVSTVETPRFKTNLEEL
NRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLSTLGKKVLYVSGEESATQIKLRADRLGVHGTELYLYPETDMDSIR
KNIELLKPDFVVIDSVQTMQEPEMTAAVGSVSQIREVTGDLMQIAKTNGITVFVVGHVTKGGAIAGPKILEHMVDTVLYF
EGDQHRTFRILRAVKNRFGSTNEIGIFEMKNGGLSEVANPSEIFLEERLKGATGSAVVASLEGTRPILVELQALVTPTAF
GNAKRTTTGLDHNRVALLMAVLEKRAGLLLQNQDAYLKAAGGVKLDEPAIDLAIAVSLVSSYRDAETRPTDCFIGEVGLT
GEIRRVNRIEQRVVEAAKLGFKRVFLPKNNLDGWMPPKEIEVVGVSTLNQALKLIFS

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=687577 M3M92_RS06050 WP_003702635.1 1237707..1239080(-) (radA) [Ligilactobacillus salivarius strain VHProbi A17]
TTGGCGAAAACTAAGACAAGATATGTTTGTCAAAATTGTGAATATATCTCTCCACGTTATTTAGGACGTTGCCCTAATTG
TGGTTCTTGGAATAGCTTGGTAGAGGAAATAGAGAAAAAGGAAAGTTCAACTAAAGCACAACCTAGAGTTAGTATCTCTG
GTACAACAGTGAAACCTAAATTAATTGATGATGTTTCTACTGTAGAAACTCCAAGATTTAAAACTAATTTAGAAGAATTG
AATCGTGTTTTAGGTGGCGGAATTGTTCCCGGATCTTTAGTGTTAATTGGTGGAGATCCAGGTATTGGCAAGTCTACATT
ATTACTACAAGTTTCTGGACAATTATCTACTCTCGGGAAAAAGGTTCTTTATGTTTCTGGGGAAGAAAGTGCAACTCAAA
TTAAATTACGTGCGGATCGTTTAGGAGTTCATGGAACTGAGCTATATTTATATCCTGAAACAGATATGGATAGTATTAGA
AAAAATATTGAACTTTTAAAGCCTGATTTTGTTGTAATCGATTCGGTGCAGACAATGCAGGAGCCAGAAATGACAGCTGC
AGTTGGAAGTGTTTCTCAAATTAGGGAAGTAACTGGAGATTTGATGCAAATTGCGAAAACTAATGGGATAACTGTCTTTG
TTGTAGGACATGTTACTAAAGGTGGAGCAATTGCAGGGCCTAAAATTTTAGAACACATGGTAGATACTGTTCTTTATTTT
GAGGGAGATCAACATAGAACATTTAGAATTTTACGAGCTGTTAAAAATAGATTTGGTTCAACTAATGAAATTGGAATCTT
TGAAATGAAAAATGGAGGATTAAGCGAAGTTGCTAATCCATCAGAGATTTTTCTAGAAGAAAGATTAAAAGGGGCTACGG
GATCAGCTGTCGTTGCTTCTCTTGAAGGAACTAGACCCATATTGGTAGAATTACAAGCACTAGTAACACCGACTGCATTT
GGAAATGCTAAGCGAACAACAACTGGTTTAGATCACAATCGAGTTGCGTTACTGATGGCAGTACTTGAAAAAAGAGCTGG
TTTGTTATTGCAAAATCAGGATGCTTATTTGAAGGCTGCTGGTGGAGTGAAGTTAGATGAGCCAGCAATAGATTTAGCCA
TTGCTGTTAGTTTAGTTTCTAGTTATCGCGATGCAGAAACAAGACCAACTGACTGTTTTATAGGAGAAGTGGGATTAACT
GGTGAAATAAGACGAGTTAATCGTATTGAACAGCGTGTTGTTGAGGCTGCTAAGCTAGGATTTAAGCGAGTATTTTTACC
AAAGAACAACTTAGATGGTTGGATGCCTCCAAAAGAGATTGAAGTAGTAGGCGTAAGTACACTAAATCAAGCATTAAAAC
TAATTTTTAGTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F5VC94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.49

100

0.685
  radA Streptococcus mitis SK321

68.271

100

0.683
  radA Streptococcus pneumoniae Rx1

68.271

100

0.683
  radA Streptococcus pneumoniae D39

68.271

100

0.683
  radA Streptococcus pneumoniae R6

68.271

100

0.683
  radA Streptococcus pneumoniae TIGR4

68.271

100

0.683
  radA Bacillus subtilis subsp. subtilis str. 168

65.563

99.125

0.65