Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   M2M89_RS12460 Genome accession   NZ_CP097130
Coordinates   2446743..2447510 (-) Length   255 a.a.
NCBI ID   WP_014477369.1    Uniprot ID   A0A0D1L7G7
Organism   Bacillus subtilis strain S16     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2441743..2452510
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2M89_RS12440 ppsR 2441917..2442729 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  M2M89_RS12445 ccpN 2442755..2443393 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  M2M89_RS12450 glyS 2443526..2445565 (-) 2040 WP_128441734.1 glycine--tRNA ligase subunit beta -
  M2M89_RS12455 glyQ 2445558..2446445 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  M2M89_RS12460 recO 2446743..2447510 (-) 768 WP_014477369.1 DNA repair protein RecO Machinery gene
  M2M89_RS12465 yqzL 2447547..2447690 (-) 144 WP_003230053.1 YqzL family protein -
  M2M89_RS12470 era 2447838..2448743 (-) 906 WP_003230051.1 GTPase Era -
  M2M89_RS12475 cdd 2448724..2449134 (-) 411 WP_003230049.1 cytidine deaminase -
  M2M89_RS12480 unpK 2449253..2449624 (-) 372 WP_046160604.1 diacylglycerol kinase family protein -
  M2M89_RS12485 ybeY 2449605..2450078 (-) 474 WP_032722131.1 rRNA maturation RNase YbeY -
  M2M89_RS12490 pgpH 2450079..2452214 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29342.05 Da        Isoelectric Point: 8.3080

>NTDB_id=687441 M2M89_RS12460 WP_014477369.1 2446743..2447510(-) (recO) [Bacillus subtilis strain S16]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=687441 M2M89_RS12460 WP_014477369.1 2446743..2447510(-) (recO) [Bacillus subtilis strain S16]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGTCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGTATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1L7G7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.608

100

0.996