Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   M2M89_RS01965 Genome accession   NZ_CP097130
Coordinates   374858..375307 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain S16     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 369858..380307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2M89_RS01930 yckA 370009..370689 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  M2M89_RS01935 yckB 370699..371562 (-) 864 WP_080121173.1 transporter substrate-binding domain-containing protein -
  M2M89_RS01940 - 371723..371920 (+) 198 WP_120363256.1 hypothetical protein -
  M2M89_RS01945 yckC 371940..372395 (+) 456 WP_024572178.1 RDD family protein -
  M2M89_RS01950 yckD 372477..372809 (+) 333 WP_003246247.1 YckD family protein -
  M2M89_RS01955 bglC 372963..374396 (+) 1434 WP_038828490.1 6-phospho-beta-glucosidase -
  M2M89_RS01960 nin/comJ 374433..374831 (-) 399 WP_021481824.1 DNA-entry nuclease inhibitor Regulator
  M2M89_RS01965 nucA/comI 374858..375307 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  M2M89_RS01970 tlpC 375475..377196 (-) 1722 WP_021481821.1 methyl-accepting chemotaxis protein TlpC -
  M2M89_RS01975 hxlB 377306..377863 (-) 558 WP_041850679.1 6-phospho-3-hexuloisomerase -
  M2M89_RS01980 hxlA 377869..378501 (-) 633 WP_041850680.1 3-hexulose-6-phosphate synthase -
  M2M89_RS01985 hxlR 378733..379095 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=687398 M2M89_RS01965 WP_014475770.1 374858..375307(-) (nucA/comI) [Bacillus subtilis strain S16]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=687398 M2M89_RS01965 WP_014475770.1 374858..375307(-) (nucA/comI) [Bacillus subtilis strain S16]
GTGAACATCACGACGGACATCATAAAAACAATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987