Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M3M40_RS04355 Genome accession   NZ_CP097119
Coordinates   826822..828204 (-) Length   460 a.a.
NCBI ID   WP_252766255.1    Uniprot ID   A0A9Q8ZS08
Organism   Fructilactobacillus cliffordii strain KI4_B1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 821822..833204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3M40_RS04330 (M3M40_04330) - 821982..822509 (-) 528 WP_252766250.1 NYN domain-containing protein -
  M3M40_RS04335 (M3M40_04335) rlmB 822538..823299 (-) 762 WP_252766251.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  M3M40_RS04340 (M3M40_04340) - 823289..823714 (-) 426 WP_252766252.1 Mini-ribonuclease 3 -
  M3M40_RS04345 (M3M40_04345) cysS 823717..825132 (-) 1416 WP_252766253.1 cysteine--tRNA ligase -
  M3M40_RS04350 (M3M40_04350) gltX 825244..826737 (-) 1494 WP_252766254.1 glutamate--tRNA ligase -
  M3M40_RS04355 (M3M40_04355) radA 826822..828204 (-) 1383 WP_252766255.1 DNA repair protein RadA Machinery gene
  M3M40_RS04360 (M3M40_04360) - 828220..828756 (-) 537 WP_252766256.1 dUTP diphosphatase -
  M3M40_RS04365 (M3M40_04365) rpiA 828902..829588 (+) 687 WP_252766257.1 ribose-5-phosphate isomerase RpiA -
  M3M40_RS04370 (M3M40_04370) - 829640..830974 (+) 1335 WP_252766258.1 C1 family peptidase -
  M3M40_RS04375 (M3M40_04375) - 831098..831769 (-) 672 WP_252766259.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  M3M40_RS04380 (M3M40_04380) - 831940..833091 (+) 1152 WP_252766260.1 LCP family protein -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49675.70 Da        Isoelectric Point: 5.1174

>NTDB_id=687330 M3M40_RS04355 WP_252766255.1 826822..828204(-) (radA) [Fructilactobacillus cliffordii strain KI4_B1]
MSKTKTQFVCSNCGYISPTYLGRCPNCGEWNTFTEETVTKDSGTNAKSTRVSLTGGRVEPQLINEVDAQDAPRYQIQSAE
LNRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLATLGKRVLYVTGEESADQVKMRADRLQIAANDRLYVFPETDMTA
VRDAIANLKPDVVIVDSVQTMQESDVDSAIGSVSQVRGVTTDLMNIAKTNNITVFIVGHVTKGGAIAGPKTLEHMVDTVL
YFEGDKHHSYRLLRAVKNRFGSTNELGIFEMADGGLQEVQNPSEIFLEERLKNATGSAIVVAMEGTRPILVELQALITPS
VFGNAQRTATGVDRNRISLIMAVLEKRAGLMLQNQDAYVKAAGGVKLNEPAIDLAMAVSIASSYENISTNPRECYVGELG
LTGEVRRVDRMEQRIREAAKLGFDRIVVPQHSLEGLDVPDGIEVIGVTTLEEALKLAIPK

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=687330 M3M40_RS04355 WP_252766255.1 826822..828204(-) (radA) [Fructilactobacillus cliffordii strain KI4_B1]
ATGAGTAAAACAAAAACCCAGTTTGTCTGTTCTAACTGCGGTTACATTTCACCGACCTACCTGGGCCGGTGCCCCAATTG
TGGGGAATGGAATACGTTTACCGAAGAAACGGTCACAAAGGATAGTGGAACAAACGCCAAGTCGACTCGAGTGTCTTTGA
CGGGGGGTCGCGTAGAACCGCAACTGATTAACGAGGTTGACGCTCAGGACGCACCCCGCTACCAGATTCAATCAGCCGAG
TTAAATCGGGTGCTCGGGGGCGGGATTGTGCCCGGATCCCTGGTTTTAATCGGGGGAGATCCCGGAATCGGGAAGTCGAC
CTTACTGCTCCAAGTTTCCGGTCAATTAGCCACACTCGGAAAACGGGTCTTGTACGTGACCGGAGAAGAAAGTGCGGATC
AGGTCAAGATGCGGGCGGATCGGTTGCAAATTGCGGCAAACGACCGGCTCTACGTCTTTCCAGAAACTGATATGACGGCG
GTGCGCGATGCAATTGCAAACCTAAAACCGGACGTCGTGATTGTTGACTCCGTTCAAACGATGCAGGAGAGCGACGTGGA
CTCCGCGATTGGCTCGGTCTCACAGGTCCGGGGTGTAACGACCGACCTGATGAACATTGCGAAGACCAACAACATCACGG
TCTTTATCGTGGGCCACGTGACCAAGGGCGGTGCAATTGCCGGACCGAAAACCCTGGAACACATGGTGGATACGGTGCTG
TACTTTGAAGGTGACAAACACCACTCGTACCGTTTGTTACGGGCGGTCAAAAATCGGTTTGGCTCGACCAACGAACTCGG
CATCTTTGAGATGGCGGACGGGGGCCTGCAAGAGGTGCAAAATCCGTCGGAGATTTTCCTAGAGGAACGGCTGAAGAATG
CGACCGGATCAGCCATTGTGGTGGCGATGGAAGGAACCCGGCCGATTTTGGTGGAACTCCAGGCCCTGATTACGCCCTCG
GTCTTTGGGAACGCACAGCGAACCGCGACCGGAGTGGATCGGAACCGCATCTCGTTGATCATGGCAGTCCTAGAAAAGCG
AGCCGGCTTGATGCTGCAAAATCAAGATGCCTACGTCAAGGCCGCTGGGGGCGTGAAATTGAACGAACCCGCGATTGATC
TGGCGATGGCGGTCAGCATTGCCTCCAGTTACGAAAACATTAGTACCAATCCACGCGAGTGTTATGTTGGAGAGCTCGGC
CTGACCGGAGAAGTGCGGCGGGTCGATCGCATGGAACAACGAATTCGGGAGGCCGCTAAGTTGGGCTTTGACCGCATCGT
GGTTCCCCAACACAGCTTGGAAGGGCTTGACGTTCCTGATGGCATCGAAGTAATCGGAGTGACGACCCTTGAGGAAGCAC
TAAAGTTGGCCATTCCCAAGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)

AAA domain (AAA_25)

(74-224)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

64.835

98.913

0.641
  radA Streptococcus pneumoniae D39

64.835

98.913

0.641
  radA Streptococcus pneumoniae R6

64.835

98.913

0.641
  radA Streptococcus pneumoniae TIGR4

64.835

98.913

0.641
  radA Streptococcus mitis NCTC 12261

64.835

98.913

0.641
  radA Streptococcus mitis SK321

64.835

98.913

0.641
  radA Bacillus subtilis subsp. subtilis str. 168

60.48

99.565

0.602