Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M3J23_RS02385 Genome accession   NZ_CP097113
Coordinates   508168..509532 (+) Length   454 a.a.
NCBI ID   WP_001085220.1    Uniprot ID   -
Organism   Staphylococcus aureus strain JP1250     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 503168..514532
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3J23_RS02365 (M3J23_02360) - 503170..503631 (+) 462 WP_000551762.1 CtsR family transcriptional regulator -
  M3J23_RS02370 (M3J23_02365) - 503650..504216 (+) 567 WP_000882069.1 UvrB/UvrC motif-containing protein -
  M3J23_RS02375 (M3J23_02370) - 504206..505213 (+) 1008 WP_000149503.1 protein arginine kinase -
  M3J23_RS02380 (M3J23_02375) - 505227..507683 (+) 2457 WP_000897132.1 ATP-dependent Clp protease ATP-binding subunit -
  M3J23_RS02385 (M3J23_02380) radA 508168..509532 (+) 1365 WP_001085220.1 DNA repair protein RadA Machinery gene
  M3J23_RS02390 (M3J23_02385) - 509557..510630 (+) 1074 WP_001028376.1 PIN/TRAM domain-containing protein -
  M3J23_RS02395 (M3J23_02390) gltX 511187..512641 (+) 1455 WP_001283792.1 glutamate--tRNA ligase -
  M3J23_RS02400 (M3J23_02395) cysE 513064..513711 (+) 648 WP_001805237.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49863.41 Da        Isoelectric Point: 7.0049

>NTDB_id=687143 M3J23_RS02385 WP_001085220.1 508168..509532(+) (radA) [Staphylococcus aureus strain JP1250]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPKHGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEVLSFALHS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=687143 M3J23_RS02385 WP_001085220.1 508168..509532(+) (radA) [Staphylococcus aureus strain JP1250]
TTGGCCAAGAAAAAAGTGATTTTTGAATGTATGGCTTGTGGTTATCAATCTCCTAAATGGATGGGGAAATGTCCTAATTG
TGGCGCTTGGAATCAAATGGAGGAAATTGTTGAAAAAGCAGCCAATCCTAAACATGGAGTTAAAACCAAGGAATTAGCAG
GTAAAGTACAAAAATTAAATAGTATTAAACATGAAACAACGCCGAGAGTGTTAACAGATTCAGCAGAATTCAACCGTGTA
TTAGGTGGAGGTATTGTGAGCGGATCGTTAGTACTTATTGGTGGGGATCCAGGTATTGGTAAGTCAACGTTACTTTTACA
AATTTGTGCATCGTTATCTCAAAAGAAAAAAGTACTATATATTACTGGAGAAGAATCGCTTAGTCAGACTAAATTACGTG
CAGAGCGATTAGATGAAGATTCAAGTGAATTGCAAGTATTAGCTGAAACAGATCTTGAAGTTATTTATCAAACAGTAAAA
GAAGAACAACCTGATTTATTAGTAGTGGATTCGATTCAAACAATATATCATCCTGAAATCAGCTCTGCGCCAGGTTCTGT
TTCACAAGTTCGTGAAAGTACACAAAGTTTAATGAATATTGCTAAACAAATGAACATTGCAACTTTTATAGTGGGTCATG
TAACGAAAGAAGGTCAAATTGCTGGCCCAAGATTGCTAGAACACATGGTTGATACTGTGCTTTATTTTGAAGGCGATGAA
CACCACGCATATCGAATTTTGCGAGCTGTTAAAAACCGTTTTGGTTCAACGAATGAAATGGGAATCTTCGAAATGAAGCA
AAGTGGATTAAAAGGTGTAAATAATCCATCTGAAATGTTTTTAGAAGAACGTTCAACAAATGTTCCAGGTTCAACAATTG
TTGCAACCATGGAGGGAACCAGACCACTTTTAATAGAAGTTCAAGCGCTGGTAACTCCAACGACTTTTAACAATCCGAGA
CGAATGGCAACAGGGATTGATCATAATCGATTAAGTTTGTTGATGGCTGTTTTGGAAAAGAAAGAAAATTATCTATTACA
ACAACAAGATGCTTATATCAAAGTAGCTGGCGGTGTAAAGTTAACGGAGCCAGCAGTTGATTTAAGTGTAATTGTAGCAA
CTGCATCTAGCTTTAAAGATAAAGCTGTCGACGGATTAGATTGCTATATTGGAGAAGTTGGTTTAACGGGTGAGGTACGT
CGTGTATCTCGGATAGAACAACGCGTGCAAGAGGCTGCAAAACTAGGTTTCAAACGTGTAATTATTCCTAAAAATAATAT
AGGCGGATGGACATATCCTGAAGGTATACAAGTAATAGGTGTAACTACTGTACATGAAGTATTGTCATTTGCTCTTCATT
CATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(77-218)

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.449

100

0.672
  radA Streptococcus pneumoniae Rx1

62.277

98.678

0.615
  radA Streptococcus pneumoniae D39

62.277

98.678

0.615
  radA Streptococcus pneumoniae R6

62.277

98.678

0.615
  radA Streptococcus pneumoniae TIGR4

62.277

98.678

0.615
  radA Streptococcus mitis NCTC 12261

62.277

98.678

0.615
  radA Streptococcus mitis SK321

62.277

98.678

0.615