Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   KK0981_RS10025 Genome accession   NZ_AP017971
Coordinates   1864603..1865427 (-) Length   274 a.a.
NCBI ID   WP_001036790.1    Uniprot ID   A0A0B7KWT3
Organism   Streptococcus pneumoniae strain KK0981     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1859603..1870427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KK0981_RS10020 (KK0981_39570) - 1862981..1864312 (-) 1332 WP_000390042.1 hemolysin family protein -
  KK0981_RS10025 (KK0981_39580) endA 1864603..1865427 (-) 825 WP_001036790.1 DNA-entry nuclease EndA Machinery gene
  KK0981_RS10030 (KK0981_39590) - 1865466..1865654 (-) 189 WP_001036519.1 DNA-directed RNA polymerase subunit beta -
  KK0981_RS10035 (KK0981_39600) murA 1865647..1866930 (-) 1284 WP_000358028.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  KK0981_RS10040 (KK0981_39610) - 1866954..1867184 (-) 231 WP_000250384.1 DUF1146 family protein -
  KK0981_RS10045 (KK0981_39620) sepM 1867242..1868279 (-) 1038 WP_001842811.1 SepM family pheromone-processing serine protease Regulator
  KK0981_RS10050 (KK0981_39630) coaD 1868263..1868751 (-) 489 WP_001280753.1 pantetheine-phosphate adenylyltransferase -
  KK0981_RS10055 (KK0981_39640) rsmD 1868741..1869280 (-) 540 WP_000706959.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  KK0981_RS10060 (KK0981_39650) asnA 1869345..1870337 (-) 993 WP_000747990.1 aspartate--ammonia ligase -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29949.69 Da        Isoelectric Point: 10.1700

>NTDB_id=68708 KK0981_RS10025 WP_001036790.1 1864603..1865427(-) (endA) [Streptococcus pneumoniae strain KK0981]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQAPEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=68708 KK0981_RS10025 WP_001036790.1 1864603..1865427(-) (endA) [Streptococcus pneumoniae strain KK0981]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGACAGCTAATAGTCCTAAAACCAAGCTTAGTCAGAAAAAACAAGCGCCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7KWT3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

98.175

100

0.982
  endA Streptococcus pneumoniae D39

98.175

100

0.982
  endA Streptococcus pneumoniae R6

98.175

100

0.982
  endA Streptococcus pneumoniae TIGR4

98.175

100

0.982

Multiple sequence alignment