Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexB   Type   Machinery gene
Locus tag   ORF34_RS09295 Genome accession   NZ_CP112873
Coordinates   1780146..1782029 (+) Length   627 a.a.
NCBI ID   WP_003245099.1    Uniprot ID   P49850
Organism   Bacillus subtilis strain O4     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1775146..1787029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ORF34_RS09280 (ORF34_09280) ymcA 1776183..1776614 (+) 432 WP_003231834.1 regulatory iron-sulfur-containing complex subunit RicA Regulator
  ORF34_RS09285 (ORF34_09285) cotE 1776876..1777421 (+) 546 WP_003231833.1 outer spore coat protein CotE -
  ORF34_RS09290 (ORF34_09290) hexA 1777554..1780130 (+) 2577 WP_003244841.1 DNA mismatch repair protein MutS Machinery gene
  ORF34_RS09295 (ORF34_09295) hexB 1780146..1782029 (+) 1884 WP_003245099.1 DNA mismatch repair endonuclease MutL Machinery gene
  ORF34_RS09300 (ORF34_09300) ymzD 1782427..1782882 (-) 456 WP_003231830.1 regulatory YrvL family protein -
  ORF34_RS09305 (ORF34_09305) - 1783037..1783594 (-) 558 WP_003245720.1 hypothetical protein -
  ORF34_RS09310 (ORF34_09310) pksA 1783715..1784332 (+) 618 WP_003231827.1 TetR/AcrR family transcriptional regulator -
  ORF34_RS09315 (ORF34_09315) pksB 1784522..1785199 (+) 678 WP_003231824.1 MBL fold metallo-hydrolase -
  ORF34_RS09320 (ORF34_09320) - 1785309..1785575 (+) 267 WP_019712693.1 hypothetical protein -
  ORF34_RS09325 (ORF34_09325) fabD 1785572..1786438 (+) 867 WP_003231821.1 ACP S-malonyltransferase -

Sequence

Protein


Download         Length: 627 a.a.        Molecular weight: 70431.38 Da        Isoelectric Point: 5.8680

>NTDB_id=686985 ORF34_RS09295 WP_003245099.1 1780146..1782029(+) (hexB) [Bacillus subtilis strain O4]
MAKVIQLSDELSNKIAAGEVVERPASVVKELVENAIDADSTVIEIDIEEAGLASIRVLDNGEGMENEDCKRAFRRHATSK
IKDENDLFRVRTLGFRGEALPSIASVSHLEITTSTGEGAGTKLVLQGGNIISESRSSSRKGTEIVVSNLFFNTPARLKYM
KTVHTELGNITDVVNRIALAHPEVSIRLRHHGKNLLQTNGNGDVRHVLAAIYGTAVAKKMLPLHVSSLDFEVKGYIALPE
ITRASRNYMSSVVNGRYIKNFPLVKAVHEGYHTLLPIGRHPITFIEITMDPILVDVNVHPSKLEVRLSKETELHDLIRDG
IKDVFKQQQLIPSAQVPKKSAPAIKNEQQFITFDEKPPEKKVPEKSTAPSYSPMKLSSVVKEPVDAEEKLPPLQFDAPPI
VDQEQTLEVSDVSAEQPETFEQECHEEQPQPASDRVPIMYPIGQMHGTYILAQNENGLYIIDQHAAQERIKYEYFREKVG
EVEPEVQEMIVPLTFHYSTNEALIIEQHKQELESVGVFLESFGSNSYIVRCHPAWFPKGEEAELIEEIIQQVLDSKNIDI
KKLREEAAIMMSCKGSIKANRHLRNDEIKALLDDLRSTSDPFTCPHGRPIIIHHSTYEMEKMFKRVM

Nucleotide


Download         Length: 1884 bp        

>NTDB_id=686985 ORF34_RS09295 WP_003245099.1 1780146..1782029(+) (hexB) [Bacillus subtilis strain O4]
GTGGCAAAAGTCATCCAACTGTCAGATGAGCTTTCAAATAAAATAGCGGCGGGCGAGGTTGTGGAACGGCCCGCCTCAGT
CGTCAAAGAATTGGTGGAAAATGCGATCGACGCTGACAGCACAGTCATTGAAATCGATATTGAGGAAGCAGGCCTTGCAT
CCATTCGGGTCTTGGATAACGGCGAAGGAATGGAGAATGAAGATTGCAAGCGAGCTTTCCGGCGCCACGCAACGAGTAAA
ATAAAAGATGAAAATGATTTATTCAGGGTGAGAACGCTCGGTTTCAGGGGAGAAGCCCTGCCGAGTATCGCGTCAGTCTC
TCATCTGGAGATTACAACAAGCACTGGTGAAGGAGCTGGGACGAAGCTCGTCCTCCAAGGAGGAAACATCATTTCCGAAT
CGCGTTCCTCAAGCAGGAAGGGAACTGAAATTGTCGTTTCCAACCTGTTTTTTAACACACCGGCCCGTTTGAAATATATG
AAAACCGTTCATACAGAGCTTGGAAACATTACAGATGTGGTTAACCGTATTGCTCTGGCGCATCCAGAGGTATCGATCCG
CCTGCGCCATCATGGGAAAAACCTGCTTCAAACGAACGGAAACGGAGATGTGCGCCATGTACTGGCAGCGATTTACGGCA
CGGCTGTCGCCAAAAAAATGCTTCCGCTGCATGTGAGCTCCTTGGATTTTGAAGTAAAGGGATATATCGCCCTCCCAGAG
ATTACACGGGCGTCGAGAAACTATATGTCGTCTGTGGTCAATGGCCGTTACATTAAAAATTTCCCGCTCGTCAAAGCGGT
TCATGAAGGCTATCATACGCTTCTGCCGATCGGACGCCATCCGATTACTTTTATAGAAATTACGATGGACCCGATTTTAG
TCGATGTCAACGTGCATCCATCGAAGCTTGAGGTCCGGCTCAGCAAGGAAACAGAGCTTCATGACTTAATACGTGACGGA
ATTAAAGATGTGTTTAAACAGCAGCAGCTGATTCCTAGTGCCCAGGTCCCGAAAAAATCAGCACCTGCCATCAAAAATGA
GCAGCAGTTTATAACCTTTGATGAAAAGCCTCCGGAAAAAAAAGTTCCGGAAAAATCGACTGCGCCATCTTATTCACCGA
TGAAGCTCAGCTCAGTCGTAAAAGAGCCGGTTGATGCGGAGGAAAAATTGCCCCCTCTCCAATTTGATGCTCCTCCTATC
GTTGATCAGGAACAAACCCTTGAGGTGTCCGATGTTTCAGCAGAACAGCCTGAAACATTTGAGCAGGAATGCCATGAGGA
ACAGCCGCAGCCAGCGTCTGATCGGGTTCCAATTATGTACCCGATTGGCCAGATGCACGGGACTTATATATTGGCACAAA
ACGAAAACGGCCTATATATTATCGACCAGCACGCCGCCCAAGAACGTATTAAATATGAGTACTTCCGTGAAAAGGTGGGG
GAGGTTGAGCCTGAGGTGCAGGAGATGATTGTACCGCTGACGTTCCACTACTCCACAAATGAGGCTTTGATTATTGAACA
GCACAAACAAGAGCTCGAAAGCGTCGGTGTCTTTTTAGAGTCATTCGGCTCTAACAGTTACATCGTCCGCTGCCACCCAG
CCTGGTTTCCAAAGGGAGAAGAAGCAGAGCTTATAGAAGAAATCATTCAGCAGGTGCTCGACTCCAAAAATATAGATATT
AAAAAACTTCGCGAGGAAGCGGCGATTATGATGAGCTGCAAAGGCTCCATCAAAGCAAATCGCCACCTCAGAAACGACGA
AATCAAAGCGCTTCTGGACGACCTCCGAAGCACATCAGACCCATTTACATGCCCGCACGGCCGCCCGATCATCATTCACC
ACTCGACATATGAGATGGAAAAGATGTTCAAACGCGTGATGTAG

Domains

Predicted by InterProScan.

DNA mismatch repair protein, C-terminal domain (DNA_mis_repair)

(209-324)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c_3)

(25-124)

MutL C terminal dimerisation domain (MutL_C)

(442-583)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 3GAB
  PDB 3KDG
  PDB 3KDK
  PDB 6E8D

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexB Streptococcus pneumoniae R6

45.497

100

0.467