Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M2908_RS07185 Genome accession   NZ_CP097045
Coordinates   1497754..1499124 (+) Length   456 a.a.
NCBI ID   WP_168413290.1    Uniprot ID   A0AAE9XII1
Organism   Vagococcus lutrae strain AT32     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1492754..1504124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2908_RS07160 (M2908_07160) - 1493934..1494653 (+) 720 WP_202585573.1 pseudouridine synthase -
  M2908_RS07165 (M2908_07165) - 1494767..1495081 (+) 315 WP_023607060.1 rhodanese-like domain-containing protein -
  M2908_RS07170 (M2908_07170) - 1495082..1496728 (+) 1647 WP_202585574.1 FAD-dependent oxidoreductase -
  M2908_RS07175 (M2908_07175) - 1496743..1497000 (+) 258 WP_126762350.1 metal-sensing transcriptional repressor -
  M2908_RS07180 (M2908_07180) - 1497116..1497649 (+) 534 WP_222316881.1 dUTP diphosphatase -
  M2908_RS07185 (M2908_07185) radA 1497754..1499124 (+) 1371 WP_168413290.1 DNA repair protein RadA Machinery gene
  M2908_RS07190 (M2908_07190) - 1499143..1500273 (+) 1131 WP_126762357.1 PIN/TRAM domain-containing protein -
  M2908_RS07195 (M2908_07195) gltX 1500369..1501835 (+) 1467 WP_248852085.1 glutamate--tRNA ligase -
  M2908_RS07200 (M2908_07200) epsC 1502042..1502647 (+) 606 WP_272158218.1 serine O-acetyltransferase EpsC -
  M2908_RS07205 (M2908_07205) cysS 1502631..1504049 (+) 1419 WP_126762363.1 cysteine--tRNA ligase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50116.58 Da        Isoelectric Point: 6.1973

>NTDB_id=686589 M2908_RS07185 WP_168413290.1 1497754..1499124(+) (radA) [Vagococcus lutrae strain AT32]
MAKKQRTQFECQACGYISPKYLGRCPNCGAWNEMMEVKIQEEPDRHSRVSLAGKRTTAKRLTEVAMEKEPRVKTQMDELN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSQQLNQADGVVLYVSGEESAEQIKMRAERLGIKGNDFYIYPETDMSQIRQ
TIEELQPDYVIIDSIQTMMQPEIASASGSVSQVRETTAELMQIAKTNHIAIFIVGHVTKEGALAGPRMLEHMVDTVLYFE
GDRHHTFRILRAVKNRFGSTNEIGIFEMVESGLQEVLNPSEVFLEERLDGATGSAIVVSMEGSRPILAEVQALVSPTLFG
NAKRTATGLDYNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLDEPAIDLAIAMSIASSYKDTGTSATECFIGEIGLTG
EVRRVNRIDQRIREAQKLGFKKVYVPKNNLHGYTPPADIEVVGIATLSEALRKVFP

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=686589 M2908_RS07185 WP_168413290.1 1497754..1499124(+) (radA) [Vagococcus lutrae strain AT32]
GTGGCGAAAAAGCAACGTACACAATTTGAATGTCAAGCATGCGGTTACATTTCTCCTAAATATTTAGGTAGATGCCCTAA
CTGCGGTGCTTGGAATGAAATGATGGAAGTGAAGATACAAGAAGAACCTGACCGTCATAGTCGCGTCAGCCTAGCTGGCA
AGCGAACAACGGCTAAGCGATTAACAGAAGTGGCGATGGAAAAAGAACCCCGTGTAAAAACACAAATGGACGAGCTTAAT
CGTGTGTTGGGTGGTGGCGTTGTTCCCGGGTCACTTGTTTTAATTGGTGGAGACCCAGGGATTGGGAAATCTACGTTATT
ACTACAAGTTTCGCAACAGCTTAATCAAGCTGATGGCGTAGTTTTATACGTGTCTGGTGAGGAAAGTGCCGAGCAGATAA
AAATGCGTGCGGAGCGTTTAGGGATTAAAGGCAATGATTTTTATATTTATCCAGAAACAGATATGTCTCAGATACGTCAA
ACTATCGAGGAATTACAACCAGACTATGTCATCATTGATTCCATCCAGACGATGATGCAACCAGAGATAGCCAGTGCATC
AGGTAGTGTGAGCCAAGTCCGAGAAACGACTGCTGAGTTGATGCAAATTGCTAAAACTAATCATATTGCTATTTTTATCG
TAGGCCATGTGACAAAGGAAGGCGCCTTAGCAGGTCCTAGAATGTTAGAACATATGGTAGATACGGTGTTGTATTTTGAA
GGGGATCGTCATCATACCTTTCGAATTTTGCGAGCGGTAAAAAACCGTTTTGGATCGACAAATGAGATTGGGATATTTGA
GATGGTGGAGTCGGGCTTACAGGAAGTCTTAAATCCTTCTGAAGTCTTCTTAGAAGAGCGATTAGACGGTGCGACTGGTT
CTGCGATTGTGGTGTCGATGGAAGGCAGTCGTCCGATTTTAGCAGAAGTGCAAGCTCTTGTATCTCCTACTTTATTTGGA
AACGCAAAGCGGACAGCGACTGGGTTAGATTATAACCGGGTGTCACTGATTATGGCAGTGTTAGAAAAACGAGCCGGTCT
TTTGTTGCAAAATCAAGATGCGTATTTAAAAGCTGCTGGTGGTGTTAAGCTAGATGAACCGGCGATTGACTTAGCCATTG
CGATGAGTATCGCTTCGAGCTACAAAGATACGGGAACTAGTGCTACCGAGTGTTTTATTGGCGAAATTGGTTTGACCGGT
GAAGTACGACGGGTGAATCGGATTGATCAACGCATTAGAGAAGCTCAAAAGTTAGGCTTTAAAAAAGTGTACGTACCAAA
AAACAATTTACATGGATATACACCGCCAGCTGATATTGAAGTGGTTGGAATTGCCACCTTATCAGAAGCGTTAAGAAAAG
TTTTTCCTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-34)

AAA domain (AAA_25)

(71-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.066

99.781

0.739
  radA Streptococcus mitis SK321

74.066

99.781

0.739
  radA Streptococcus pneumoniae Rx1

73.846

99.781

0.737
  radA Streptococcus pneumoniae D39

73.846

99.781

0.737
  radA Streptococcus pneumoniae R6

73.846

99.781

0.737
  radA Streptococcus pneumoniae TIGR4

73.846

99.781

0.737
  radA Bacillus subtilis subsp. subtilis str. 168

66.15

99.123

0.656