Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M2919_RS07425 Genome accession   NZ_CP097017
Coordinates   1547542..1548912 (+) Length   456 a.a.
NCBI ID   WP_248848525.1    Uniprot ID   -
Organism   Vagococcus lutrae strain AT46b     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1542542..1553912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2919_RS07400 (M2919_07400) - 1543722..1544441 (+) 720 WP_248848521.1 pseudouridine synthase -
  M2919_RS07405 (M2919_07405) - 1544555..1544869 (+) 315 WP_023607060.1 rhodanese-like domain-containing protein -
  M2919_RS07410 (M2919_07410) - 1544870..1546516 (+) 1647 WP_248848522.1 FAD-dependent oxidoreductase -
  M2919_RS07415 (M2919_07415) - 1546552..1546788 (+) 237 WP_248848523.1 metal-sensing transcriptional repressor -
  M2919_RS07420 (M2919_07420) - 1546904..1547437 (+) 534 WP_248848524.1 dUTP diphosphatase -
  M2919_RS07425 (M2919_07425) radA 1547542..1548912 (+) 1371 WP_248848525.1 DNA repair protein RadA Machinery gene
  M2919_RS07430 (M2919_07430) - 1548931..1550061 (+) 1131 WP_126762357.1 PIN/TRAM domain-containing protein -
  M2919_RS07435 (M2919_07435) gltX 1550157..1551623 (+) 1467 WP_126762359.1 glutamate--tRNA ligase -
  M2919_RS07440 (M2919_07440) epsC 1551830..1552435 (+) 606 WP_272158218.1 serine O-acetyltransferase EpsC -
  M2919_RS07445 (M2919_07445) cysS 1552419..1553837 (+) 1419 WP_248848526.1 cysteine--tRNA ligase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50102.55 Da        Isoelectric Point: 6.1967

>NTDB_id=686234 M2919_RS07425 WP_248848525.1 1547542..1548912(+) (radA) [Vagococcus lutrae strain AT46b]
MAKKQRTQFECQACGYISPKYLGRCPNCGAWNEMMEVKIQEEPDRHSRVSLAGKRTTAKRLTEVAMEKEPRVKTQMDELN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSQQLNQADGVVLYVSGEESAEQIKMRAERLGIKGNDFYIYPETDMSQIRQ
TIEDLQPDYVIIDSIQTMMQPEIASASGSVSQVRETTAELMQIAKTNHIAIFIVGHVTKEGALAGPRMLEHMVDTVLYFE
GDRHHTFRILRAVKNRFGSTNEIGIFEMVESGLQEVLNPSEVFLEERLDGATGSAIVVSMEGSRPILAEVQALVSPTLFG
NAKRTATGLDYNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLDEPAIDLAIAMSIASSYKDTGTSATECFIGEIGLTG
EVRRVNRIDQRIREAQKLGFKKVYVPKNNLHGYTPPADIEVVGIATLSEALRKVFP

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=686234 M2919_RS07425 WP_248848525.1 1547542..1548912(+) (radA) [Vagococcus lutrae strain AT46b]
GTGGCAAAAAAGCAACGTACACAATTTGAATGTCAAGCATGCGGTTACATTTCTCCTAAATATTTAGGTAGATGCCCTAA
CTGCGGTGCTTGGAATGAAATGATGGAAGTGAAGATACAAGAAGAACCTGACCGTCATAGTCGCGTCAGCCTAGCTGGCA
AGCGAACAACGGCTAAGCGATTAACAGAAGTCGCGATGGAAAAAGAACCCCGTGTAAAAACACAAATGGACGAGCTTAAT
CGTGTGTTGGGTGGTGGCGTTGTTCCCGGGTCACTTGTTTTAATTGGTGGAGACCCAGGAATTGGGAAATCTACGTTGTT
ACTACAAGTTTCGCAACAGCTTAATCAAGCTGATGGCGTAGTTTTATACGTGTCTGGTGAGGAAAGTGCCGAGCAGATAA
AAATGCGTGCGGAGCGTTTAGGGATTAAAGGCAATGATTTTTATATTTATCCAGAAACAGATATGTCTCAAATACGTCAA
ACTATCGAAGACTTACAACCAGACTATGTCATCATTGATTCCATCCAGACGATGATGCAACCGGAGATAGCCAGTGCATC
AGGTAGTGTGAGTCAAGTCCGAGAAACGACTGCTGAGTTAATGCAAATCGCTAAAACTAATCATATTGCTATTTTTATCG
TAGGCCATGTGACAAAGGAAGGCGCCTTAGCAGGTCCTAGAATGTTAGAACATATGGTAGATACGGTGTTGTATTTTGAA
GGGGATCGTCATCATACCTTTCGAATTTTGCGAGCGGTAAAAAACCGTTTTGGATCGACAAATGAGATTGGGATATTTGA
GATGGTGGAGTCGGGATTACAGGAAGTCTTAAATCCTTCTGAAGTCTTCTTAGAAGAGCGATTAGATGGTGCGACTGGCT
CGGCGATTGTGGTGTCGATGGAAGGCAGTCGTCCGATTTTAGCAGAAGTTCAAGCTCTTGTATCTCCTACTTTATTTGGA
AACGCAAAGCGGACAGCGACTGGGTTAGATTATAACCGGGTGTCACTGATTATGGCAGTGTTAGAAAAACGAGCCGGTCT
TTTGTTGCAAAATCAAGATGCGTATTTAAAAGCTGCTGGTGGTGTTAAGCTAGATGAACCGGCGATTGACTTAGCCATTG
CGATGAGTATCGCTTCGAGCTACAAAGATACGGGAACTAGTGCTACCGAGTGTTTTATTGGCGAAATTGGTTTGACCGGT
GAAGTACGACGGGTGAATCGGATTGATCAACGCATTAGAGAAGCTCAAAAGTTAGGCTTTAAAAAAGTGTACGTACCAAA
AAACAATTTACATGGATATACACCGCCAGCTGATATTGAAGTGGTTGGAATTGCCACCTTATCAGAAGCGCTAAGAAAAG
TTTTTCCTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-34)

AAA domain (AAA_25)

(71-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.066

99.781

0.739
  radA Streptococcus mitis SK321

74.066

99.781

0.739
  radA Streptococcus pneumoniae Rx1

73.846

99.781

0.737
  radA Streptococcus pneumoniae D39

73.846

99.781

0.737
  radA Streptococcus pneumoniae R6

73.846

99.781

0.737
  radA Streptococcus pneumoniae TIGR4

73.846

99.781

0.737
  radA Bacillus subtilis subsp. subtilis str. 168

65.929

99.123

0.654