Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M2925_RS00185 Genome accession   NZ_CP097004
Coordinates   43094..44464 (+) Length   456 a.a.
NCBI ID   WP_002356050.1    Uniprot ID   -
Organism   Enterococcus faecalis strain LS05-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 38094..49464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2925_RS00160 (M2925_00160) - 38584..39051 (-) 468 WP_002383505.1 VOC family protein -
  M2925_RS00165 (M2925_00165) - 39116..40063 (+) 948 WP_002419440.1 YafY family protein -
  M2925_RS00170 (M2925_00170) - 40155..41408 (-) 1254 WP_219259778.1 glutamate-5-semialdehyde dehydrogenase -
  M2925_RS00175 (M2925_00175) proB 41395..42210 (-) 816 WP_010714974.1 glutamate 5-kinase -
  M2925_RS00180 (M2925_00180) - 42530..43015 (+) 486 WP_002361472.1 dUTP diphosphatase -
  M2925_RS00185 (M2925_00185) radA 43094..44464 (+) 1371 WP_002356050.1 DNA repair protein RadA Machinery gene
  M2925_RS00190 (M2925_00190) - 44565..45710 (+) 1146 WP_002383500.1 PIN/TRAM domain-containing protein -
  M2925_RS00195 (M2925_00195) ispF 45729..46202 (+) 474 WP_002367570.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  M2925_RS00200 (M2925_00200) gltX 46255..47712 (+) 1458 WP_010706819.1 glutamate--tRNA ligase -
  M2925_RS00205 (M2925_00205) epsC 47982..48521 (+) 540 WP_002356054.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49757.28 Da        Isoelectric Point: 7.3489

>NTDB_id=686028 M2925_RS00185 WP_002356050.1 43094..44464(+) (radA) [Enterococcus faecalis strain LS05-2]
MAKKAKVQFECQSCGYVSPKYLGRCPNCGQWNSMVEEVIQDTSDRRARVSLTGKKTQPQRLSEVVPKKEPRVKTELVELN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSQQLAATGGTVLYVSGEESAEQIKLRAERLGTVNETFYLYAETDMHEISR
AIEKLEPDYVIIDSIQTMTQPDVTSVAGSVSQVRETTAELLKLAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GDKHHTFRILRAVKNRFGSTNEIGIFEMQTHGLVEVMNPSQVFLEERLEGATGSSIVVAMEGSRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKINEPAIDLALAISIASSYKEKGTSSSECFIGEIGLTG
EIRRVNSIEQRVREAQKLGFTKVYVPKNNLGGWEAPEGIEIIGVSTIGETLRKVFK

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=686028 M2925_RS00185 WP_002356050.1 43094..44464(+) (radA) [Enterococcus faecalis strain LS05-2]
ATGGCAAAAAAAGCAAAAGTTCAATTTGAATGTCAAAGCTGTGGGTATGTGTCTCCAAAATATCTCGGCCGCTGTCCTAA
TTGTGGACAATGGAATTCGATGGTGGAAGAGGTTATTCAAGATACTTCTGATCGTCGTGCTAGAGTAAGTTTGACTGGTA
AAAAGACACAACCACAACGGCTATCTGAAGTCGTACCTAAAAAAGAGCCTCGTGTAAAAACTGAATTAGTGGAGCTGAAT
CGGGTTTTAGGTGGTGGCGTTGTCCCAGGTTCGTTAGTTTTAATCGGTGGCGATCCCGGAATTGGTAAGTCAACATTGCT
CTTACAAGTATCGCAACAATTAGCAGCCACAGGTGGCACGGTTTTATATGTTTCTGGTGAAGAAAGTGCTGAGCAAATTA
AATTACGAGCGGAGCGTCTAGGCACCGTCAACGAGACATTTTACTTATACGCAGAAACAGATATGCACGAAATATCACGA
GCCATTGAAAAATTAGAACCAGATTATGTCATTATTGACTCGATTCAAACAATGACGCAGCCAGATGTTACCAGCGTTGC
TGGTAGTGTCAGTCAAGTCCGAGAAACAACTGCCGAATTATTGAAACTGGCAAAAACGAATGGGATTGCCATTTTTATTG
TCGGACACGTAACGAAGGAAGGGTCTATCGCAGGGCCACGGATGTTGGAACATATGGTAGACACGGTTCTTTATTTTGAA
GGAGATAAGCATCACACCTTTAGAATTTTACGGGCTGTCAAAAATCGGTTTGGCTCAACGAATGAGATTGGTATTTTTGA
AATGCAGACACATGGATTGGTTGAAGTTATGAATCCTTCTCAGGTCTTTTTAGAAGAACGTTTAGAAGGAGCCACTGGGT
CTTCGATTGTGGTAGCAATGGAAGGTTCACGCCCCATTTTGGTGGAAATCCAAGCGTTAGTTACCCCAACGATGTTTGGC
AATGCCAAGCGAACGACCACCGGGTTGGACTTTAATCGCGTTTCTTTAATCATGGCGGTTTTAGAAAAACGGGCAGGCCT
TTTATTACAAAACCAGGATGCGTATCTAAAAGCGGCTGGTGGAGTCAAAATTAATGAACCAGCTATTGATTTAGCGTTAG
CAATCAGTATTGCTTCTAGCTATAAAGAAAAAGGAACCTCATCTTCAGAATGTTTCATTGGTGAGATTGGTTTAACTGGT
GAAATCCGCCGCGTGAATAGTATTGAACAACGAGTGCGTGAAGCGCAAAAACTAGGCTTTACAAAAGTCTATGTCCCTAA
AAATAACCTTGGTGGCTGGGAAGCGCCTGAAGGGATTGAAATTATTGGCGTTTCAACAATTGGTGAAACGTTAAGAAAAG
TTTTTAAATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(65-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

75.385

99.781

0.752
  radA Streptococcus pneumoniae Rx1

75.385

99.781

0.752
  radA Streptococcus pneumoniae D39

75.385

99.781

0.752
  radA Streptococcus pneumoniae R6

75.385

99.781

0.752
  radA Streptococcus pneumoniae TIGR4

75.385

99.781

0.752
  radA Streptococcus mitis SK321

75.385

99.781

0.752
  radA Bacillus subtilis subsp. subtilis str. 168

66.297

98.904

0.656