NTDB

Overview

Name	radA	Type	Machinery gene
Locus tag	CLFE_RS03590	Genome accession	NZ_CP096980
Coordinates	767685..769037 (+)	Length	450 a.a.
NCBI ID	WP_077833993.1	Uniprot ID	-
Organism	Clostridium felsineum DSM 794
Function	homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 762685..774037

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CLFE_RS03570 (CLFE_007170)	-	763007..763468 (+)	462	WP_077892327.1	CtsR family transcriptional regulator	-
CLFE_RS03575 (CLFE_007180)	-	763493..764020 (+)	528	WP_077833996.1	UvrB/UvrC motif-containing protein	-
CLFE_RS03580 (CLFE_007190)	-	764022..765044 (+)	1023	WP_077892328.1	protein arginine kinase	-
CLFE_RS03585 (CLFE_007200)	-	765055..767505 (+)	2451	WP_077892329.1	ATP-dependent Clp protease ATP-binding subunit	-
CLFE_RS03590 (CLFE_007210)	radA	767685..769037 (+)	1353	WP_077833993.1	DNA repair protein RadA	Machinery gene
CLFE_RS03595 (CLFE_007220)	disA	769049..770122 (+)	1074	WP_077833992.1	DNA integrity scanning diadenylate cyclase DisA	-
CLFE_RS03600 (CLFE_007230)	-	770159..770557 (-)	399	WP_077833991.1	DUF1573 domain-containing protein	-
CLFE_RS03605 (CLFE_007240)	-	770947..772044 (+)	1098	WP_077833990.1	PIN/TRAM domain-containing protein	-
CLFE_RS03610 (CLFE_007250)	ispD	772068..772748 (+)	681	WP_077892330.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
CLFE_RS03615 (CLFE_007260)	-	772804..773769 (-)	966	WP_077892331.1	ABC transporter ATP-binding protein	-

Sequence

Protein

Download Length: 450 a.a. Molecular weight: 49876.63 Da Isoelectric Point: 8.4368

                                >NTDB_id=685871 CLFE_RS03590 WP_077833993.1 767685..769037(+) (radA) [Clostridium felsineum DSM 794]

MSKIKSYFVCQECGYQSVKWLGKCPSCNNWNSFIEEEKVENKKTSKIILNSEPKCINNIKSGQYERYDTNNKELNRVLGG

GLVKGSITLISGAPGIGKSTLLLQICQDIAVKYGKVLYVSGEESENQIKMRGDRLKVTSPNIYILSETNMENIEEHVNNI

KPAFVIIDSIQTLYKEQITSAPGSVSQVRECANDIMRIAKTSNIPFFIVAHVTKQGELAGPRVLEHIVDTVLTFEGQRTE

EFRILRTVKNRFGTTSEIGVFEMQEEGLKQIYNPSGLFLEETNFNQEGSVVIGIMEGTRPILVEIQALVSKTNAVMPRRT

SVGIDSSRLNLILAVLEKKLKVPFYNCDVYVNVVGGLNIEGTYGDLGLALALISSVKGKSIKLDKLIVIGEIGLTGEVRP

VSFSDRIVNEADKLGFKNVLVPHKNIKSDKNVAINTIGVSYLKEAVNKVF

Nucleotide

Download Length: 1353 bp

                                >NTDB_id=685871 CLFE_RS03590 WP_077833993.1 767685..769037(+) (radA) [Clostridium felsineum DSM 794]

ATGAGCAAAATTAAAAGTTATTTTGTATGTCAGGAATGTGGATATCAGTCAGTAAAATGGCTTGGAAAATGTCCATCTTG

TAATAACTGGAATAGCTTTATAGAGGAAGAAAAGGTAGAAAATAAAAAGACAAGTAAAATAATATTAAATAGTGAACCCA

AATGTATAAATAATATAAAATCAGGACAGTATGAAAGATATGATACAAATAATAAAGAGCTAAATAGAGTACTTGGTGGA

GGACTTGTTAAAGGATCAATTACATTAATATCAGGGGCACCTGGTATAGGAAAGTCCACACTCCTTCTACAAATTTGTCA

GGATATAGCAGTGAAATACGGAAAAGTGCTTTATGTTTCAGGAGAAGAGTCTGAAAATCAAATAAAAATGCGTGGAGATA

GATTAAAAGTAACCTCTCCTAATATATATATACTATCAGAAACTAATATGGAAAATATAGAAGAGCATGTCAATAATATA

AAGCCTGCCTTTGTTATAATAGATTCAATACAAACATTGTATAAAGAACAAATAACTTCTGCTCCAGGCAGTGTTTCTCA

AGTTAGAGAATGTGCGAATGATATTATGAGAATAGCCAAAACTAGTAATATACCATTTTTTATAGTAGCACATGTAACAA

AGCAGGGGGAACTTGCAGGACCAAGAGTATTAGAACATATTGTAGATACGGTGCTTACATTTGAAGGACAAAGAACAGAA

GAATTTAGGATACTTAGGACAGTTAAAAATCGTTTTGGTACTACAAGTGAAATTGGTGTATTTGAAATGCAAGAAGAAGG

ACTTAAACAAATATATAATCCTTCAGGGCTATTTCTAGAGGAAACAAATTTTAATCAAGAAGGTTCAGTTGTAATAGGCA

TTATGGAAGGAACAAGACCAATACTTGTTGAGATACAAGCGTTAGTTTCAAAAACAAATGCAGTAATGCCAAGAAGAACA

TCTGTTGGAATTGATAGTTCTAGACTTAATTTAATTTTAGCAGTTCTTGAAAAAAAGCTTAAAGTTCCATTTTATAATTG

TGATGTTTACGTAAATGTAGTAGGCGGATTAAATATTGAAGGAACCTATGGAGATTTAGGATTAGCACTTGCTTTAATTT

CAAGTGTTAAAGGTAAAAGTATAAAGTTAGATAAGCTGATAGTTATAGGTGAAATTGGATTAACAGGAGAAGTAAGACCA

GTATCTTTTTCAGATAGAATTGTGAATGAAGCGGATAAATTAGGATTTAAAAATGTATTAGTTCCACACAAAAATATTAA

AAGTGATAAAAATGTTGCGATAAATACAATAGGTGTGTCTTATTTAAAGGAAGCTGTAAATAAAGTTTTTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(60-216)

Subunit ChlI of Mg-chelatase (ChlI)

(340-425)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
radA	Bacillus subtilis subsp. subtilis str. 168	50.331	100	0.507
radA	Streptococcus mitis SK321	49.119	100	0.496
radA	Streptococcus mitis NCTC 12261	49.119	100	0.496
radA	Streptococcus pneumoniae Rx1	49.119	100	0.496
radA	Streptococcus pneumoniae D39	49.119	100	0.496
radA	Streptococcus pneumoniae R6	49.119	100	0.496
radA	Streptococcus pneumoniae TIGR4	49.119	100	0.496

Detailed information NTG0685871