Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CCX78_RS00990 Genome accession   NZ_AP017931
Coordinates   190674..192038 (+) Length   454 a.a.
NCBI ID   WP_096584176.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain LK-145     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 185674..197038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCX78_RS00970 (LASAK_00181) - 185994..187340 (-) 1347 WP_096584170.1 C1 family peptidase -
  CCX78_RS00975 (LASAK_00182) - 187544..188866 (-) 1323 WP_076639104.1 aminopeptidase C -
  CCX78_RS00980 (LASAK_00183) rpiA 189180..189866 (-) 687 WP_096584173.1 ribose-5-phosphate isomerase RpiA -
  CCX78_RS00985 (LASAK_00184) - 190062..190601 (+) 540 WP_011375374.1 dUTP diphosphatase -
  CCX78_RS00990 (LASAK_00185) radA 190674..192038 (+) 1365 WP_096584176.1 DNA repair protein RadA Machinery gene
  CCX78_RS00995 (LASAK_00186) - 192069..193181 (+) 1113 WP_035146631.1 PIN/TRAM domain-containing protein -
  CCX78_RS01000 (LASAK_00187) cysS 193849..195255 (+) 1407 WP_096584178.1 cysteine--tRNA ligase -
  CCX78_RS01005 (LASAK_00188) - 195252..195659 (+) 408 WP_004265086.1 Mini-ribonuclease 3 -
  CCX78_RS01010 (LASAK_00189) rlmB 195646..196533 (+) 888 WP_082267747.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49611.03 Da        Isoelectric Point: 7.3488

>NTDB_id=68578 CCX78_RS00990 WP_096584176.1 190674..192038(+) (radA) [Latilactobacillus sakei strain LK-145]
MAKVKTQFVCQNCGYSSPRFLGRCPNCGAWNQMVEEREQPAAAAKSNFTISGRATEPEKISTVNIQKEPRVKTELNELNR
VLGGGVVPGSLILIGGDPGIGKSTLLLQVSGQLEKVGKILYVSGEESASQIKMRANRLGVNGDQLYLYPETDMGNIRHQI
ETLKPEYVVIDSIQTMSEPEVTSAVGSVSQVRQVTAELMRIAKTNQITIFVVGHVTKEGAIAGPKILEHMVDTVLYFEGD
THHIYRILRSVKNRFGSTNEIGIFEMREAGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVELQTLITPTLFGNA
KRTSSGLDHNRVSLIMAVLEKRASLMLSNQDAYLKATGGVKLDEPAIDLAMAVSIASSYRDKEIPPTDCFVGEIGLTGEI
RRVNRIEQRVGEAAKLGFKRIYVPKNNLQGWDPPTDIQVVGVTTIAETLKKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=68578 CCX78_RS00990 WP_096584176.1 190674..192038(+) (radA) [Latilactobacillus sakei strain LK-145]
GTGGCTAAAGTTAAAACGCAGTTTGTCTGCCAGAATTGTGGCTATAGTTCCCCCAGATTCTTGGGGCGCTGTCCGAATTG
TGGTGCTTGGAACCAAATGGTTGAAGAACGTGAACAACCAGCCGCAGCGGCAAAATCAAATTTCACAATTTCAGGGCGCG
CCACTGAACCTGAAAAAATTAGTACTGTTAATATCCAAAAAGAACCCCGTGTTAAAACGGAATTAAATGAATTGAATCGT
GTTTTAGGTGGCGGCGTTGTTCCGGGTTCACTGATTTTAATCGGTGGGGATCCTGGGATTGGAAAATCAACCTTGCTACT
ACAAGTTTCAGGGCAACTTGAAAAAGTGGGTAAGATCCTATACGTTTCAGGTGAAGAAAGTGCCTCACAAATTAAGATGC
GGGCGAATCGATTAGGGGTTAATGGCGATCAATTGTATCTGTATCCCGAAACGGATATGGGTAATATTCGCCACCAAATT
GAAACGCTCAAACCAGAATACGTCGTGATTGATTCGATTCAAACGATGAGTGAACCAGAAGTGACTTCCGCAGTCGGGAG
TGTTTCGCAAGTTCGCCAAGTAACCGCAGAATTGATGCGGATTGCTAAAACGAATCAAATTACCATCTTCGTGGTGGGAC
ATGTGACGAAGGAAGGGGCGATTGCCGGCCCTAAGATTTTGGAACATATGGTGGATACCGTGTTGTACTTTGAAGGTGAC
ACCCATCACATTTACCGGATTTTACGATCTGTTAAAAACCGGTTTGGTTCAACCAATGAAATCGGGATTTTCGAAATGCG
GGAAGCGGGCTTGCAAGAAGTCGCTAATCCATCCGAAATTTTCCTTGAAGAACGACTAGCCGGTGCGACTGGCTCAGCAG
TTGTGGTTTCAATGGAAGGGACAAGACCAATCTTGGTTGAGTTACAAACTTTGATTACCCCAACCTTGTTCGGGAATGCG
AAACGGACCTCTTCAGGGTTAGATCATAATCGGGTGTCATTGATTATGGCGGTTTTAGAAAAGCGGGCGAGTTTGATGCT
TTCAAACCAAGATGCTTATCTTAAAGCAACCGGTGGCGTTAAGTTAGATGAACCAGCGATTGATTTGGCGATGGCCGTTT
CAATTGCTTCCAGTTATCGCGATAAAGAAATTCCACCAACCGATTGTTTTGTCGGCGAAATTGGCTTGACGGGTGAAATC
CGGCGGGTCAACCGGATCGAACAACGCGTTGGCGAAGCGGCGAAGTTAGGTTTTAAACGGATTTATGTTCCTAAAAATAA
TTTGCAAGGTTGGGATCCACCAACTGATATTCAAGTCGTTGGGGTCACCACTATTGCAGAAACTTTAAAAAAAGTATTCA
ACTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

AAA domain (AAA_25)

(70-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

69.095

99.78

0.689
  radA Streptococcus mitis NCTC 12261

69.095

99.78

0.689
  radA Streptococcus pneumoniae Rx1

68.874

99.78

0.687
  radA Streptococcus pneumoniae D39

68.874

99.78

0.687
  radA Streptococcus pneumoniae R6

68.874

99.78

0.687
  radA Streptococcus pneumoniae TIGR4

68.874

99.78

0.687
  radA Bacillus subtilis subsp. subtilis str. 168

64.901

99.78

0.648

Multiple sequence alignment