Detailed information NTG0685776

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M2J01_RS24815
• Genome accession: NZ_CP096964
• Coordinates: 5351275..5352147 (+)
• Length: 290 a.a.
• NCBI ID: WP_034001500.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain NY13936
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5346275..5357147

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M2J01_RS24800 (M2J01_24750)	-	5347658..5348122 (-)	465	WP_003122079.1	pilin	-
M2J01_RS24805 (M2J01_24755)	pilB	5348353..5350053 (+)	1701	WP_003122078.1	type IV-A pilus assembly ATPase PilB	Machinery gene
M2J01_RS24810 (M2J01_24760)	pilC	5350057..5351274 (+)	1218	WP_003107299.1	type II secretion system F family protein	Machinery gene
M2J01_RS24815 (M2J01_24765)	pilD	5351275..5352147 (+)	873	WP_034001500.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
M2J01_RS24820 (M2J01_24770)	coaE	5352144..5352755 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
M2J01_RS24825 (M2J01_24775)	yacG	5352752..5352952 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
M2J01_RS24830 (M2J01_24780)	-	5352989..5353198 (-)	210	WP_003094660.1	hypothetical protein	-
M2J01_RS24835 (M2J01_24785)	-	5353304..5353993 (-)	690	WP_003094662.1	energy-coupling factor ABC transporter permease	-
M2J01_RS24840 (M2J01_24790)	-	5353990..5354460 (-)	471	WP_003094664.1	hypothetical protein	-
M2J01_RS24845 (M2J01_24795)	-	5354457..5354882 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
M2J01_RS24850 (M2J01_24800)	-	5355015..5355644 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
M2J01_RS24855 (M2J01_24805)	-	5355641..5356090 (+)	450	WP_023105574.1	MOSC domain-containing protein	-
M2J01_RS24860 (M2J01_24810)	-	5356116..5356286 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31790.83 Da
• Isoelectric Point: 7.3233

>NTDB_id=685776 M2J01_RS24815 WP_034001500.1 5351275..5352147(+) (pilD) [Pseudomonas aeruginosa strain NY13936]
MTLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMECNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=685776 M2J01_RS24815 WP_034001500.1 5351275..5352147(+) (pilD) [Pseudomonas aeruginosa strain NY13936]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGTGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTTGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.357	96.552	0.534
pilD	Vibrio campbellii strain DS40M4	53.004	97.586	0.517
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	48.921	95.862	0.469