Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   LEUCM_RS06830 Genome accession   NZ_AP017929
Coordinates   1356824..1357531 (+) Length   235 a.a.
NCBI ID   WP_226474265.1    Uniprot ID   -
Organism   Latilactobacillus sakei subsp. sakei DSM 20017 = JCM 1157 strain LT-13     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1351824..1362531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEUCM_RS06800 (LACBS_01343) - 1352031..1353218 (+) 1188 WP_025015730.1 FtsW/RodA/SpoVE family cell cycle protein -
  LEUCM_RS06805 (LACBS_01344) - 1353229..1354317 (+) 1089 WP_025015731.1 CAP-associated domain-containing protein -
  LEUCM_RS06810 (LACBS_01345) - 1354341..1354631 (+) 291 WP_016265218.1 YlbG family protein -
  LEUCM_RS06815 (LACBS_01346) rsmD 1354636..1355190 (+) 555 WP_016265217.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  LEUCM_RS06820 (LACBS_01347) coaD 1355203..1355700 (+) 498 WP_025015732.1 pantetheine-phosphate adenylyltransferase -
  LEUCM_RS06825 (LACBS_01348) - 1355702..1356748 (+) 1047 WP_035145546.1 SepM family pheromone-processing serine protease -
  LEUCM_RS06830 (LACBS_01349) comEA 1356824..1357531 (+) 708 WP_226474265.1 helix-hairpin-helix domain-containing protein Machinery gene
  LEUCM_RS06835 (LACBS_01350) - 1357589..1358077 (+) 489 WP_011374770.1 ComE operon protein 2 -
  LEUCM_RS06840 (LACBS_01351) comEC 1358275..1360341 (+) 2067 WP_231953244.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  LEUCM_RS06845 (LACBS_01352) holA 1360338..1361366 (+) 1029 WP_025015735.1 DNA polymerase III subunit delta -
  LEUCM_RS06850 (LACBS_01353) rpsT 1361434..1361688 (-) 255 WP_011374767.1 30S ribosomal protein S20 -
  LEUCM_RS06855 (LACBS_01354) rpsO 1361967..1362236 (+) 270 WP_011374766.1 30S ribosomal protein S15 -

Sequence

Protein


Download         Length: 235 a.a.        Molecular weight: 26122.75 Da        Isoelectric Point: 9.7683

>NTDB_id=68564 LEUCM_RS06830 WP_226474265.1 1356824..1357531(+) (comEA) [Latilactobacillus sakei subsp. sakei DSM 20017 = JCM 1157 strain LT-13]
MMWQDYWQRYRRFWWVPCLVLLGIITGGFYWHQTQQKQKVTHNEQLLATSASKIKPATKKTHQAATKKKGGFVDIKGAVA
YPGIYPITVSETRLFEVLKNAGGPTDAADTKRLNLAQKLTDQLIIYIPKKGEVVTDQELIQAPQQIATTSERTPEQASTG
TSANASSASDTAKIDLNQADSTQLQTLNGIGPKKAEQIIAYRDEQGKFKQIEDLQKVGGIGPKTFEQLQSQICVN

Nucleotide


Download         Length: 708 bp        

>NTDB_id=68564 LEUCM_RS06830 WP_226474265.1 1356824..1357531(+) (comEA) [Latilactobacillus sakei subsp. sakei DSM 20017 = JCM 1157 strain LT-13]
ATGATGTGGCAAGATTATTGGCAGCGATATCGGCGTTTTTGGTGGGTGCCTTGCTTAGTGCTATTGGGGATCATCACGGG
TGGCTTTTATTGGCATCAAACGCAACAAAAACAAAAAGTAACGCATAATGAGCAACTGCTAGCAACCAGTGCGTCTAAAA
TAAAACCTGCTACTAAGAAAACACACCAAGCGGCAACCAAGAAAAAAGGCGGTTTTGTCGATATAAAAGGTGCTGTGGCT
TATCCGGGAATTTATCCGATCACAGTCTCTGAAACGCGCTTATTTGAGGTGCTCAAAAATGCAGGTGGTCCGACTGATGC
GGCTGATACCAAGCGGCTTAATTTAGCCCAAAAATTAACCGATCAACTGATTATTTATATTCCGAAAAAAGGAGAGGTGG
TTACTGATCAAGAACTCATTCAAGCGCCACAACAAATTGCAACGACCAGTGAGCGTACGCCAGAACAAGCATCAACTGGG
ACAAGTGCGAATGCATCGAGCGCGAGTGACACTGCTAAAATCGATTTGAATCAGGCTGATAGTACGCAGCTTCAGACCCT
CAATGGCATTGGCCCCAAGAAAGCTGAACAAATAATTGCTTATCGTGATGAACAAGGAAAATTTAAACAGATTGAAGATC
TACAAAAAGTCGGCGGGATTGGTCCGAAGACGTTTGAACAATTGCAATCACAAATTTGTGTGAATTAG

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(73-124)

Helix-hairpin-helix motif (HHH_3)

(172-233)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Latilactobacillus sakei subsp. sakei 23K

97.021

100

0.97

Multiple sequence alignment