Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   M2I93_RS26530 Genome accession   NZ_CP096946
Coordinates   5643085..5643411 (-) Length   108 a.a.
NCBI ID   WP_349632734.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NY5530     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5623803..5642808 5643085..5643411 flank 277


Gene organization within MGE regions


Location: 5623803..5643411
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2I93_RS26420 (M2I93_26375) - 5623803..5625017 (+) 1215 WP_034069051.1 site-specific integrase -
  M2I93_RS26425 (M2I93_26380) - 5625019..5625561 (-) 543 WP_123809552.1 hypothetical protein -
  M2I93_RS26430 (M2I93_26385) - 5625705..5625905 (+) 201 WP_023082007.1 helix-turn-helix domain-containing protein -
  M2I93_RS26435 (M2I93_26390) - 5625902..5626318 (+) 417 WP_034054964.1 hypothetical protein -
  M2I93_RS26440 (M2I93_26395) - 5626311..5626544 (+) 234 WP_031653969.1 hypothetical protein -
  M2I93_RS26445 (M2I93_26400) - 5626541..5626789 (+) 249 WP_031653970.1 hypothetical protein -
  M2I93_RS26450 (M2I93_26405) - 5626786..5627091 (+) 306 WP_023109345.1 hypothetical protein -
  M2I93_RS33680 - 5627094..5627531 (+) 438 WP_308416434.1 hypothetical protein -
  M2I93_RS26460 (M2I93_26415) - 5627570..5628037 (+) 468 WP_031629313.1 hypothetical protein -
  M2I93_RS26465 (M2I93_26420) - 5628044..5628997 (+) 954 WP_308416433.1 DUF3631 domain-containing protein -
  M2I93_RS26470 (M2I93_26425) - 5628997..5629290 (+) 294 WP_221345446.1 hypothetical protein -
  M2I93_RS26475 (M2I93_26430) - 5629346..5630242 (+) 897 WP_034069047.1 hypothetical protein -
  M2I93_RS26480 (M2I93_26435) - 5630948..5632123 (+) 1176 WP_223822176.1 DUF262 domain-containing protein -
  M2I93_RS26485 (M2I93_26440) - 5632134..5632961 (+) 828 WP_124157035.1 MAE_28990/MAE_18760 family HEPN-like nuclease -
  M2I93_RS26490 (M2I93_26445) - 5634275..5634781 (-) 507 WP_034064628.1 GNAT family N-acetyltransferase -
  M2I93_RS26495 (M2I93_26450) - 5635554..5635823 (+) 270 WP_225007575.1 hypothetical protein -
  M2I93_RS26500 (M2I93_26455) - 5635823..5636059 (+) 237 WP_003096116.1 hypothetical protein -
  M2I93_RS26505 (M2I93_26460) - 5636198..5636434 (-) 237 WP_071536299.1 hypothetical protein -
  M2I93_RS26510 (M2I93_26465) - 5636462..5639224 (-) 2763 WP_057379627.1 RHS repeat domain-containing protein -
  M2I93_RS26515 (M2I93_26470) - 5639239..5640387 (-) 1149 WP_172773772.1 DUF6531 domain-containing protein -
  M2I93_RS26520 (M2I93_26475) - 5642566..5642808 (+) 243 WP_023109353.1 hypothetical protein -
  M2I93_RS26530 (M2I93_26485) pilA 5643085..5643411 (-) 327 WP_349632734.1 pilin Machinery gene

Sequence


Protein


Download         Length: 108 a.a.        Molecular weight: 11243.69 Da        Isoelectric Point: 6.2562

>NTDB_id=685534 M2I93_RS26530 WP_349632734.1 5643085..5643411(-) (pilA) [Pseudomonas aeruginosa strain NY5530]
MATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYVGIDEKANKLGTVAVDIKDTGDGTVKFTFATGQSSPKNAGTAI
TLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide


Download         Length: 327 bp        

>NTDB_id=685534 M2I93_RS26530 WP_349632734.1 5643085..5643411(-) (pilA) [Pseudomonas aeruginosa strain NY5530]
CTGGCGACGATCAACCCGCTGAAGACTACTGTTGAAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGG
TACTACAGCTTCTACTGCAGATACCACCTATGTAGGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAGATA
TTAAAGACACAGGCGATGGTACTGTAAAATTTACTTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCACGGCAATT
ACTTTGAATCGTACTGCTGAAGGTGTATGGACTTGCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAA
GCCTTAA

Domains


Predicted by InterproScan.

(1-106)


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

51.818

100

0.528

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

33.613

100

0.37

  pilA Vibrio cholerae strain A1552

33.613

100

0.37

  pilA Vibrio cholerae C6706

33.613

100

0.37