Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M2J85_RS25055 Genome accession   NZ_CP096920
Coordinates   5386402..5387772 (-) Length   456 a.a.
NCBI ID   WP_003251626.1    Uniprot ID   I7AVL1
Organism   Pseudomonas putida strain NY4811     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5381402..5392772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2J85_RS25035 (M2J85_24950) - 5381764..5381961 (-) 198 WP_003251633.1 YbdD/YjiX family protein -
  M2J85_RS25040 (M2J85_24955) - 5381979..5384045 (-) 2067 WP_004577217.1 carbon starvation CstA family protein -
  M2J85_RS25045 (M2J85_24960) - 5384223..5384585 (+) 363 WP_003251630.1 PilZ domain-containing protein -
  M2J85_RS25050 (M2J85_24965) - 5384849..5386366 (+) 1518 WP_060538251.1 nucleobase:cation symporter-2 family protein -
  M2J85_RS25055 (M2J85_24970) radA 5386402..5387772 (-) 1371 WP_003251626.1 DNA repair protein RadA Machinery gene
  M2J85_RS25060 (M2J85_24975) mscL 5387949..5388368 (+) 420 WP_003251622.1 large-conductance mechanosensitive channel protein MscL -
  M2J85_RS25065 (M2J85_24980) - 5388644..5389420 (-) 777 WP_003251619.1 ferredoxin--NADP reductase -
  M2J85_RS25070 (M2J85_24985) - 5389636..5390343 (+) 708 WP_003251617.1 autoinducer binding domain-containing protein -
  M2J85_RS25075 (M2J85_24990) - 5390440..5391564 (+) 1125 WP_003251615.1 methyltransferase -
  M2J85_RS25080 (M2J85_24995) - 5391670..5391825 (-) 156 WP_003251614.1 DUF2474 domain-containing protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 48741.18 Da        Isoelectric Point: 6.8985

>NTDB_id=685180 M2J85_RS25055 WP_003251626.1 5386402..5387772(-) (radA) [Pseudomonas putida strain NY4811]
MAKAKRLYGCTECGATFPKWAGQCGECGAWNTLVETMIESGGAAAPSSGRAGWTGQQAQIKTLAEVSVEEIPRFTTSSTE
LDRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAVGMPALYVTGEESQQQVAMRSRRLGLPQDQLKVMTETCIETII
ATARVEKPRVMVIDSIQTIFTEQLQSAPGGVAQVRESTALLVRYAKQSGTAIFLVGHVTKEGSLAGPRVLEHMVDTVLYF
EGESDGRLRLLRAVKNRFGAVNELGVFGMTDRGLKEVSNPSAIFLNRTQEEVPGSVVMATWEGTRPMLVEVQALVDDSHL
ANPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALLAAVMSSLRNRPLAHGLLVFGEIGLS
GEVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPAGLQVIAVTRLEQALDALFE

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=685180 M2J85_RS25055 WP_003251626.1 5386402..5387772(-) (radA) [Pseudomonas putida strain NY4811]
ATGGCCAAGGCCAAGCGCTTGTATGGCTGCACCGAATGCGGCGCGACCTTCCCCAAATGGGCCGGCCAGTGCGGCGAATG
CGGGGCCTGGAACACCCTGGTCGAAACCATGATCGAAAGCGGCGGCGCTGCTGCACCCAGTAGCGGCCGCGCAGGCTGGA
CAGGACAGCAGGCGCAAATCAAGACCCTGGCCGAAGTCAGTGTCGAGGAAATCCCGCGTTTCACGACCAGCAGCACCGAA
CTGGACCGCGTGCTGGGTGGCGGCTTGGTGGATGGCTCGGTGGTGCTGATTGGCGGTGACCCCGGCATCGGCAAGTCGAC
CATTTTGCTACAGACCTTGTGCAACATCGCCGTGGGCATGCCGGCACTGTATGTCACGGGGGAGGAGTCGCAGCAGCAGG
TGGCCATGCGCTCGCGGCGGCTGGGCCTACCCCAGGACCAGCTCAAGGTGATGACCGAAACCTGCATCGAGACGATCATT
GCCACAGCGCGGGTCGAGAAGCCGCGGGTAATGGTGATCGACTCGATCCAGACCATTTTCACTGAGCAGTTGCAATCGGC
CCCGGGCGGCGTGGCCCAGGTGCGCGAAAGCACAGCGTTGCTGGTGCGCTATGCCAAGCAAAGCGGTACAGCAATCTTTC
TGGTCGGCCACGTGACCAAGGAAGGCTCGCTGGCTGGCCCAAGGGTGCTGGAGCACATGGTCGACACGGTGCTGTATTTC
GAAGGTGAGTCCGATGGCCGGCTGCGCTTGCTGCGGGCCGTCAAGAACCGCTTTGGCGCGGTCAACGAGCTGGGCGTGTT
CGGCATGACCGATCGTGGCCTCAAGGAGGTATCCAACCCGTCGGCGATCTTCCTCAACCGTACTCAGGAAGAGGTGCCGG
GTAGCGTGGTGATGGCAACCTGGGAGGGTACCCGGCCAATGCTGGTCGAGGTGCAGGCGCTGGTCGACGATAGCCATCTG
GCCAACCCGCGGCGGGTAACCTTGGGCCTGGACCAGAACCGCCTGGCCATGTTGTTGGCGGTGCTCCACCGCCATGGCGG
TATCCCGACCCATGACCAGGATGTGTTTCTCAACGTGGTCGGCGGGGTGAAGGTGCTGGAAACGGCTTCGGACCTGGCGC
TGCTGGCGGCGGTGATGTCCAGCTTGCGTAACCGGCCACTGGCCCACGGTTTGCTGGTGTTTGGCGAGATTGGCTTGTCG
GGCGAGGTACGGCCGGTGCCCAGTGGTCAGGAGCGCTTGAAGGAAGCGGCCAAGCACGGCTTCAAGCGTGCCATCGTACC
CAAGGGCAATGCGCCGAAAGAAGCGCCTGCGGGTTTGCAGGTGATTGCCGTTACCCGCCTGGAACAGGCGCTGGATGCAT
TGTTCGAATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(355-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(73-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I7AVL1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.684

100

0.487
  radA Streptococcus pneumoniae Rx1

46.087

100

0.465
  radA Streptococcus pneumoniae D39

46.087

100

0.465
  radA Streptococcus pneumoniae TIGR4

46.087

100

0.465
  radA Streptococcus pneumoniae R6

46.087

100

0.465
  radA Streptococcus mitis NCTC 12261

45.87

100

0.463
  radA Streptococcus mitis SK321

46.053

100

0.461