Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   M2J84_RS17900 Genome accession   NZ_CP096918
Coordinates   3793391..3794971 (-) Length   526 a.a.
NCBI ID   WP_270149913.1    Uniprot ID   -
Organism   Comamonas aquatica strain NY8661     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3788391..3799971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2J84_RS17885 (M2J84_17900) - 3789453..3789941 (-) 489 WP_045265908.1 putative toxin-antitoxin system toxin component, PIN family -
  M2J84_RS17890 (M2J84_17905) - 3789945..3791228 (-) 1284 WP_270149909.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  M2J84_RS17895 (M2J84_17910) - 3791381..3793207 (+) 1827 WP_270149911.1 methyl-accepting chemotaxis protein -
  M2J84_RS17900 (M2J84_17915) rpoN/rpoN1 3793391..3794971 (-) 1581 WP_270149913.1 RNA polymerase factor sigma-54 Machinery gene
  M2J84_RS17905 (M2J84_17920) lptB 3795022..3795792 (-) 771 WP_270149916.1 LPS export ABC transporter ATP-binding protein -
  M2J84_RS17910 (M2J84_17925) lptA 3795792..3796430 (-) 639 WP_042417727.1 lipopolysaccharide transport periplasmic protein LptA -
  M2J84_RS17915 (M2J84_17930) - 3796534..3797181 (-) 648 WP_042417634.1 thiol:disulfide interchange protein DsbA/DsbL -
  M2J84_RS17920 (M2J84_17935) - 3797237..3797887 (-) 651 WP_270149920.1 SPOR domain-containing protein -
  M2J84_RS17925 (M2J84_17940) argS 3797918..3799627 (-) 1710 WP_270149922.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 526 a.a.        Molecular weight: 58137.52 Da        Isoelectric Point: 4.8649

>NTDB_id=685160 M2J84_RS17900 WP_270149913.1 3793391..3794971(-) (rpoN/rpoN1) [Comamonas aquatica strain NY8661]
MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDDNPFLELASDDAPREEFGLQTADEPAQHEGASVDETPY
SIADSAGAVSAEGQKDQESSAPDSDVPSDWEGDGSVDMAHDDSEWGGDAPARGHRNDDEDAPTALDLAGAHESLTDFLHQ
QALSLRLSEIDRYALRFLIENLNDDGYLEDSLQELAEGLAGTDDLEQLDELVHRFTVALRLLQSLEPVGVGARDLAECLR
LQLLHLHREDPQDEMLEVALQVCAQPLEMLARRDVRKLMQACGLPEAKVRAALGVIARLEPKPGRRFTDVERHIIVPDVI
VKKTSRKGGDGLAQFSVQLNPDVMPRLRVHDVYANALRSHKGSDGHAGLQQRLQEARWFIKNVQQRFDTILRVSKAIVDR
QKNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPHGTYELKFFFGSGLGTETGGNASSTAVRALIKQFVAA
ESPKKPLSDSQIADMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1581 bp        

>NTDB_id=685160 M2J84_RS17900 WP_270149913.1 3793391..3794971(-) (rpoN/rpoN1) [Comamonas aquatica strain NY8661]
TTGAAGCCAGGTTTGTCACTGCGGGTCTCACAGCATCTGGCGCTCACGCCGCAGTTGCAGCAATCCATCCGGCTGTTGCA
GCTGTCCACCCTGGAGTTGTCCCAGGAAGTCGAGCAGATGCTCGACGACAACCCGTTTCTGGAGCTGGCCTCGGACGACG
CCCCGCGCGAGGAGTTCGGCCTGCAGACCGCGGACGAGCCCGCCCAGCACGAGGGCGCCTCTGTCGACGAAACACCATAT
TCCATAGCAGACAGCGCAGGTGCAGTAAGCGCTGAAGGCCAAAAAGACCAAGAATCCTCGGCACCCGACAGCGATGTGCC
CAGCGACTGGGAGGGCGACGGCAGCGTTGACATGGCGCACGACGACAGCGAATGGGGTGGCGACGCCCCGGCGCGCGGCC
ACCGCAATGACGACGAGGATGCCCCCACGGCCCTGGACCTGGCGGGCGCGCACGAGTCGCTGACCGATTTCCTGCACCAG
CAGGCACTGTCGCTGCGCCTGTCCGAGATCGACCGCTACGCGCTGCGTTTCCTGATCGAAAACCTCAACGACGACGGCTA
CCTCGAGGACTCGCTGCAGGAGTTGGCCGAGGGCCTGGCCGGCACCGACGACCTGGAGCAGCTCGACGAGCTGGTGCACC
GCTTCACCGTGGCCCTGCGCCTGCTGCAGTCGCTGGAACCCGTGGGCGTGGGCGCCCGCGACCTGGCCGAATGCCTGCGC
CTGCAGCTGCTGCACCTGCACCGCGAGGACCCGCAGGACGAGATGCTGGAGGTGGCGCTGCAGGTCTGCGCGCAGCCGCT
GGAAATGCTGGCCCGGCGCGATGTGCGCAAGCTGATGCAGGCCTGCGGCCTGCCCGAGGCCAAGGTGCGCGCCGCGCTGG
GCGTCATCGCCCGGCTGGAGCCCAAGCCGGGGCGGCGCTTCACCGATGTCGAGCGCCACATCATCGTGCCCGACGTGATC
GTCAAGAAAACGTCGCGCAAGGGGGGCGATGGCCTGGCGCAGTTCAGCGTGCAGCTGAACCCGGACGTGATGCCGCGCCT
GCGCGTGCACGACGTCTACGCCAACGCCCTGCGCAGCCACAAGGGCAGCGACGGGCACGCCGGACTGCAGCAGCGGCTGC
AGGAGGCGCGCTGGTTCATCAAGAACGTGCAGCAGCGCTTCGACACCATCCTGCGCGTGTCCAAGGCCATCGTCGACCGG
CAGAAGAACTTCTTCGTGCACGGCGAGCTGGCCATGCGCCCGCTGGTGCTGCGTGACATCGCCGACGAGCTGGGCCTGCA
CGAATCGACCATCAGCCGCGTGACCACGGCCAAGTACATGGCCACGCCGCATGGCACCTACGAGCTGAAGTTCTTCTTCG
GCTCGGGCCTGGGCACGGAGACCGGGGGCAATGCCTCCAGCACCGCGGTGCGCGCGCTGATCAAGCAGTTCGTCGCGGCC
GAGAGCCCGAAGAAACCGCTGTCCGACAGCCAGATCGCGGACATGCTCAAGGAGCAGGGCATCGAGTGCGCGCGCCGCAC
CGTGGCCAAGTACCGCGAGGCCTTGAAGATCGCTCCGGCCAACCTGCGCAAGGCGCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(369-524)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(148-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.019

100

0.534