Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EFK13_RS13100 Genome accession   NZ_CP096889
Coordinates   2569780..2570190 (-) Length   136 a.a.
NCBI ID   WP_129508189.1    Uniprot ID   -
Organism   Bacillus cabrialesii strain TE3     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2564780..2575190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFK13_RS13070 (EFK13_13070) - 2564853..2564993 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  EFK13_RS21100 - 2565132..2565266 (-) 135 WP_283107553.1 hypothetical protein -
  EFK13_RS13075 (EFK13_13075) - 2565341..2566072 (-) 732 WP_129508193.1 SGNH/GDSL hydrolase family protein -
  EFK13_RS13080 (EFK13_13080) - 2566326..2567078 (-) 753 WP_129508192.1 N-acetylmuramoyl-L-alanine amidase -
  EFK13_RS13085 (EFK13_13085) - 2567273..2567899 (+) 627 WP_129508191.1 TVP38/TMEM64 family protein -
  EFK13_RS13090 (EFK13_13090) gnd 2567917..2568784 (-) 868 Protein_2535 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  EFK13_RS13095 (EFK13_13095) - 2569028..2569747 (+) 720 WP_129508190.1 DUF308 domain-containing protein -
  EFK13_RS13100 (EFK13_13100) nucA/comI 2569780..2570190 (-) 411 WP_129508189.1 sporulation-specific Dnase NucB Machinery gene
  EFK13_RS13105 (EFK13_13105) - 2570386..2570736 (+) 351 Protein_2538 sigma-70 family RNA polymerase sigma factor -
  EFK13_RS13110 (EFK13_13110) spoIVCA 2570764..2572224 (-) 1461 WP_225514266.1 site-specific DNA recombinase SpoIVCA -
  EFK13_RS13115 (EFK13_13115) - 2572182..2572360 (-) 179 Protein_2540 hypothetical protein -
  EFK13_RS13120 (EFK13_13120) - 2572678..2572920 (+) 243 WP_010330740.1 hypothetical protein -
  EFK13_RS13125 (EFK13_13125) - 2573087..2574310 (-) 1224 WP_010330741.1 MFS transporter -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14847.89 Da        Isoelectric Point: 5.1750

>NTDB_id=684890 EFK13_RS13100 WP_129508189.1 2569780..2570190(-) (nucA/comI) [Bacillus cabrialesii strain TE3]
MKKWLAGLFLAAAVLLCLMVPQQIQGASLYDKVLYFPLSRYPETGDHIKDAIAGGHSDICTIDRDGADKRREESLKGIPT
KPGFDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSGYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=684890 EFK13_RS13100 WP_129508189.1 2569780..2570190(-) (nucA/comI) [Bacillus cabrialesii strain TE3]
ATGAAAAAATGGTTGGCAGGCCTGTTTCTTGCTGCGGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
TTCTTTGTATGACAAAGTGTTGTATTTTCCGCTGTCTCGTTATCCGGAAACCGGGGATCATATTAAAGATGCGATTGCAG
GGGGACATTCAGATATTTGCACCATCGACAGGGATGGAGCAGACAAAAGACGGGAGGAATCATTAAAAGGAATTCCGACG
AAGCCGGGTTTTGATCGGGATGAGTGGCCGATGGCGGTTTGTGAGGAAGGCGGCGCAGGAGCTGACGTCCGATATGTGAC
GCCTTCTGATAATCGCGGGGCCGGCTCGTGGGTAGGGAATCAAATGAGCGGTTATCCTGACGGCACCAGGGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529