Detailed information NTG0684466

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M1V28_RS02040
• Genome accession: NZ_CP096822
• Coordinates: 443329..444477 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain NY8709
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 438329..449477

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M1V28_RS02015 (M1V28_02015)	-	439480..439776 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
M1V28_RS02020 (M1V28_02020)	-	439773..440366 (-)	594	WP_003084544.1	YggT family protein	-
M1V28_RS02025 (M1V28_02025)	proC	440377..441198 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
M1V28_RS02030 (M1V28_02030)	-	441210..441902 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
M1V28_RS02035 (M1V28_02035)	pilT	442117..443151 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
M1V28_RS02040 (M1V28_02040)	pilU	443329..444477 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
M1V28_RS02045 (M1V28_02045)	czcD	444485..445384 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
M1V28_RS02050 (M1V28_02050)	-	445538..445942 (+)	405	WP_003084560.1	NINE protein	-
M1V28_RS02055 (M1V28_02055)	-	446185..447558 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
M1V28_RS02060 (M1V28_02060)	-	447555..448739 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=684466 M1V28_RS02040 WP_003100959.1 443329..444477(+) (pilU) [Pseudomonas aeruginosa strain NY8709]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=684466 M1V28_RS02040 WP_003100959.1 443329..444477(+) (pilU) [Pseudomonas aeruginosa strain NY8709]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382