Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   HRS14_RS18545 Genome accession   NZ_AP022650
Coordinates   3753979..3754656 (+) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli strain 09-02E     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3748979..3759656
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HRS14_RS18510 (Esc0902E_35830) gspG 3749459..3749896 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  HRS14_RS18515 (Esc0902E_35840) gspH 3749904..3750413 (+) 510 WP_001539909.1 type II secretion system minor pseudopilin GspH -
  HRS14_RS18520 (Esc0902E_35850) gspI 3750410..3750787 (+) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  HRS14_RS18525 (Esc0902E_35860) gspJ 3750780..3751367 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  HRS14_RS18530 (Esc0902E_35870) gspK 3751360..3752343 (+) 984 WP_001058513.1 type II secretion system minor pseudopilin GspK -
  HRS14_RS18535 (Esc0902E_35880) gspL 3752358..3753521 (+) 1164 Protein_3680 type II secretion system protein GspL -
  HRS14_RS18540 (Esc0902E_35900) gspM 3753518..3753979 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  HRS14_RS18545 (Esc0902E_35910) pilD 3753979..3754656 (+) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  HRS14_RS18550 (Esc0902E_35920) bfr 3754685..3755161 (-) 477 WP_000675504.1 bacterioferritin -
  HRS14_RS18555 (Esc0902E_35930) bfd 3755233..3755427 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  HRS14_RS18560 (Esc0902E_35940) chiA 3755596..3758298 (-) 2703 WP_000773146.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=68443 HRS14_RS18545 WP_000178175.1 3753979..3754656(+) (pilD) [Escherichia coli strain 09-02E]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=68443 HRS14_RS18545 WP_000178175.1 3753979..3754656(+) (pilD) [Escherichia coli strain 09-02E]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387

Multiple sequence alignment