Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PALA45_RS04545 Genome accession   NZ_CP110351
Coordinates   975135..976628 (-) Length   497 a.a.
NCBI ID   WP_003094357.1    Uniprot ID   A0A0H2ZGR9
Organism   Pseudomonas aeruginosa strain PALA45     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 970135..981628
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA45_RS04510 (PALA45_00900) - 970986..971435 (+) 450 WP_003094369.1 hypothetical protein -
  PALA45_RS04515 (PALA45_00901) - 971448..972059 (+) 612 WP_003098862.1 superoxide dismutase -
  PALA45_RS04520 (PALA45_00902) - 972078..973010 (+) 933 WP_003123303.1 ZIP family metal transporter -
  PALA45_RS04525 (PALA45_00903) - 973107..973379 (-) 273 WP_003094362.1 HPr family phosphocarrier protein -
  PALA45_RS04530 (PALA45_00904) rapZ 973409..974269 (-) 861 WP_003094361.1 RNase adapter RapZ -
  PALA45_RS04535 (PALA45_00905) ptsN 974271..974735 (-) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PALA45_RS04540 (PALA45_00906) hpf 974749..975057 (-) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  PALA45_RS04545 (PALA45_00907) rpoN/rpoN1 975135..976628 (-) 1494 WP_003094357.1 RNA polymerase factor sigma-54 Machinery gene
  PALA45_RS04550 (PALA45_00908) lptB 976854..977579 (-) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  PALA45_RS04555 (PALA45_00909) lptA 977579..978106 (-) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  PALA45_RS04560 (PALA45_00910) lptC 978093..978665 (-) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  PALA45_RS04565 (PALA45_00911) - 978674..979213 (-) 540 WP_003098849.1 KdsC family phosphatase -
  PALA45_RS04570 (PALA45_00912) kdsD 979213..980193 (-) 981 WP_269974003.1 arabinose-5-phosphate isomerase KdsD -
  PALA45_RS04575 (PALA45_00914) - 980607..981416 (+) 810 WP_003094343.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56054.22 Da        Isoelectric Point: 4.4327

>NTDB_id=684359 PALA45_RS04545 WP_003094357.1 975135..976628(-) (rpoN/rpoN1) [Pseudomonas aeruginosa strain PALA45]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=684359 PALA45_RS04545 WP_003094357.1 975135..976628(-) (rpoN/rpoN1) [Pseudomonas aeruginosa strain PALA45]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCTCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCCCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAGTGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTACCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCGCGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCATGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCCGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZGR9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477