Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   M2A29_RS15105 Genome accession   NZ_CP096812
Coordinates   3222036..3222836 (-) Length   266 a.a.
NCBI ID   WP_284625743.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain 51248     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3217036..3227836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2A29_RS15085 (M2A29_15080) - 3218101..3218799 (-) 699 WP_000058788.1 tetratricopeptide repeat protein -
  M2A29_RS15090 (M2A29_15085) hisS 3218828..3220120 (-) 1293 WP_000095254.1 histidine--tRNA ligase -
  M2A29_RS15095 (M2A29_15090) ispG 3220117..3221232 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  M2A29_RS15100 (M2A29_15095) - 3221254..3222045 (-) 792 WP_000405163.1 RodZ domain-containing protein -
  M2A29_RS15105 (M2A29_15100) pilF 3222036..3222836 (-) 801 WP_284625743.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M2A29_RS15110 (M2A29_15105) rlmN 3222852..3224084 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  M2A29_RS15115 (M2A29_15110) ndk 3224207..3224638 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  M2A29_RS15120 (M2A29_15115) iscX 3224752..3224952 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  M2A29_RS15125 (M2A29_15120) - 3225052..3225603 (-) 552 WP_272292224.1 phosphatase PAP2 family protein -
  M2A29_RS15130 (M2A29_15125) - 3225628..3226926 (-) 1299 WP_086261868.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30098.43 Da        Isoelectric Point: 9.2307

>NTDB_id=684322 M2A29_RS15105 WP_284625743.1 3222036..3222836(-) (pilF) [Acinetobacter baumannii strain 51248]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKSNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRESYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=684322 M2A29_RS15105 WP_284625743.1 3222036..3222836(-) (pilF) [Acinetobacter baumannii strain 51248]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAATCCAATTTAGAAAAAGCAGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGAATCTTATATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_16)

(88-134)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.248

100

0.992
  pilF Acinetobacter baylyi ADP1

73.462

97.744

0.718