Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   OFY13_RS01815 Genome accession   NZ_CP110188
Coordinates   370332..371795 (-) Length   487 a.a.
NCBI ID   WP_000804830.1    Uniprot ID   Q9KP49
Organism   Vibrio cholerae strain E1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 365332..376795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OFY13_RS01785 (OFY13_01785) pmbA 365473..366816 (+) 1344 WP_000382642.1 metalloprotease PmbA -
  OFY13_RS01790 (OFY13_01790) mgtE 366889..368250 (-) 1362 WP_000840452.1 magnesium transporter -
  OFY13_RS01795 (OFY13_01795) - 368411..368689 (-) 279 WP_001135899.1 HPr family phosphocarrier protein -
  OFY13_RS01800 (OFY13_01800) rapZ 368698..369558 (-) 861 WP_001237198.1 RNase adapter RapZ -
  OFY13_RS01805 (OFY13_01805) ptsN 369571..370017 (-) 447 WP_001177163.1 PTS IIA-like nitrogen regulatory protein PtsN -
  OFY13_RS01810 (OFY13_01810) hpf 370020..370307 (-) 288 WP_001166643.1 ribosome hibernation promoting factor -
  OFY13_RS01815 (OFY13_01815) rpoN/rpoN1 370332..371795 (-) 1464 WP_000804830.1 RNA polymerase factor sigma-54 Machinery gene
  OFY13_RS01820 (OFY13_01820) lptB 371851..372576 (-) 726 WP_000996180.1 LPS export ABC transporter ATP-binding protein -
  OFY13_RS01825 (OFY13_01825) lptA 372579..373073 (-) 495 WP_000830444.1 lipopolysaccharide transport periplasmic protein LptA -
  OFY13_RS01830 (OFY13_01830) lptC 373054..373617 (-) 564 WP_000059348.1 LPS export ABC transporter periplasmic protein LptC -
  OFY13_RS01835 (OFY13_01835) kdsC 373614..374171 (-) 558 WP_000099768.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  OFY13_RS01840 (OFY13_01840) kdsD 374171..375145 (-) 975 WP_001120615.1 arabinose-5-phosphate isomerase KdsD -
  OFY13_RS01845 (OFY13_01845) - 375201..376163 (-) 963 WP_000904329.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 487 a.a.        Molecular weight: 55438.47 Da        Isoelectric Point: 4.2824

>NTDB_id=683397 OFY13_RS01815 WP_000804830.1 370332..371795(-) (rpoN/rpoN1) [Vibrio cholerae strain E1]
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALESNPLLEVEENQDEAPSLDSVRMTEESPREPEELYEPEP
QDSSDLIEKSEISAELEMDTTWDEVYSANTGSTGLALDDDAPIYQGETTQTLQDYLHWQLDLTPFSDVDRTIAVALIDAI
DDYGYLTVSLEEIQESLRSDDIELDEIEAVRKRIQQFDPFGVASLNLQDCLLLQLTTYPCDTPWLEEARLLLSQYIDDLG
NRDYKTILKETKLKEEDLREILQLIQQLDPRPGSRIAQDHAEYVIPDVSVYKEQGRWLVTINPDSVPKLKINQQYADLMR
GNNAESNYIRTNLQEAKWLIKSLESRNETLLKVAKCIVEHQHDFFEYGEEAMKPMVLNDVAMAVEMHESTISRVTTQKYM
HTPRGIFELKYFFSSHVSTDNGGECSSTAIRALIKKLVAAENPAKPLSDSKIATLLADQGIQVARRTIAKYRESLGIAPS
SQRKRLL

Nucleotide


Download         Length: 1464 bp        

>NTDB_id=683397 OFY13_RS01815 WP_000804830.1 370332..371795(-) (rpoN/rpoN1) [Vibrio cholerae strain E1]
ATGAAACCGTCATTACAGCTCAAGCTAGGTCAACAGTTAGCCATGACTCCGCAGCTCCAGCAGGCGATTCGTCTGTTGCA
GCTTTCGACGCTGGATCTGCAGCAAGAAATTCAGGAAGCATTGGAATCTAACCCCTTGTTAGAGGTGGAAGAGAACCAAG
ACGAAGCGCCATCGCTTGATAGCGTACGTATGACGGAAGAAAGCCCGCGCGAGCCAGAAGAACTCTACGAACCTGAACCG
CAGGATAGCTCGGATCTGATTGAAAAATCGGAAATCAGCGCTGAATTAGAGATGGATACCACTTGGGATGAAGTCTACAG
CGCCAATACTGGTAGTACAGGACTGGCACTCGATGATGATGCCCCTATCTACCAAGGCGAAACCACCCAAACTCTGCAAG
ATTATCTTCACTGGCAACTCGACTTAACTCCCTTTAGCGATGTGGATCGCACTATAGCCGTTGCACTTATCGATGCCATC
GACGATTACGGTTATTTAACCGTCTCACTAGAGGAGATCCAAGAGAGCCTGCGCAGTGATGACATTGAGTTGGATGAGAT
TGAAGCGGTACGTAAACGCATTCAGCAGTTTGACCCCTTTGGTGTGGCATCGCTTAATCTGCAAGACTGCTTGTTGCTGC
AACTCACCACCTATCCCTGTGATACTCCTTGGCTGGAAGAAGCGCGTTTACTGCTCTCACAGTACATCGATGATTTAGGG
AATCGCGATTACAAAACCATTCTGAAAGAGACTAAGCTCAAAGAAGAGGACTTGCGCGAGATCCTGCAACTCATCCAACA
GCTCGATCCTCGCCCCGGCAGTCGAATTGCCCAAGATCACGCTGAATACGTTATACCTGACGTGTCAGTCTATAAAGAAC
AAGGTCGATGGCTGGTCACCATCAACCCAGATAGTGTGCCTAAACTGAAGATCAATCAGCAGTATGCCGACCTGATGCGC
GGTAATAATGCGGAGAGCAACTACATCCGTACCAATTTGCAAGAGGCAAAATGGCTGATCAAAAGTTTAGAAAGTAGAAA
CGAGACACTGCTCAAAGTTGCCAAGTGCATAGTTGAACACCAACACGATTTCTTCGAGTATGGAGAAGAGGCGATGAAGC
CGATGGTGCTCAACGATGTGGCGATGGCGGTGGAAATGCATGAATCGACAATTTCGCGTGTCACCACGCAGAAATACATG
CATACCCCGCGCGGCATTTTTGAGTTGAAGTACTTTTTCTCCAGCCACGTCAGTACTGACAATGGCGGAGAGTGCTCGTC
CACTGCGATCAGGGCGCTGATCAAAAAACTGGTGGCGGCAGAGAATCCTGCCAAACCACTGAGTGACAGCAAGATCGCAA
CCCTACTCGCTGACCAAGGCATCCAAGTCGCGCGGCGAACCATCGCCAAATATCGTGAATCACTTGGCATCGCCCCATCA
AGTCAGCGCAAACGCCTGCTATAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(327-485)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KP49

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.533

100

0.46