Detailed information NTG0683078

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M1S14_RS15030
• Genome accession: NZ_CP096717
• Coordinates: 3084138..3085256 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain 5741
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3079138..3090256

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M1S14_RS15020 (M1S14_15020)	-	3082254..3082946 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
M1S14_RS15025 (M1S14_15025)	pilT	3083073..3084110 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
M1S14_RS15030 (M1S14_15030)	pilU	3084138..3085256 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
M1S14_RS15035 (M1S14_15035)	fur	3085326..3085763 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
M1S14_RS15040 (M1S14_15040)	bamE	3085875..3086273 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
M1S14_RS15045 (M1S14_15045)	-	3086328..3086651 (-)	324	WP_000442587.1	RnfH family protein	-
M1S14_RS15050 (M1S14_15050)	-	3086651..3087718 (-)	1068	WP_002019572.1	hypothetical protein	-
M1S14_RS15055 (M1S14_15055)	-	3087871..3088323 (-)	453	WP_249374761.1	bacteriohemerythrin	-
M1S14_RS15060 (M1S14_15060)	-	3088576..3089424 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=683078 M1S14_RS15030 WP_000347039.1 3084138..3085256(+) (pilU) [Acinetobacter baumannii strain 5741]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=683078 M1S14_RS15030 WP_000347039.1 3084138..3085256(+) (pilU) [Acinetobacter baumannii strain 5741]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395