Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   OLR76_RS16985 Genome accession   NZ_CP110017
Coordinates   3441828..3443261 (+) Length   477 a.a.
NCBI ID   WP_282983251.1    Uniprot ID   -
Organism   Escherichia coli strain HE_50     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3436828..3448261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OLR76_RS16955 (OLR76_16865) yrbG 3437386..3438363 (+) 978 WP_000922901.1 calcium/sodium antiporter -
  OLR76_RS16960 (OLR76_16870) kdsD 3438377..3439363 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  OLR76_RS16965 (OLR76_16875) kdsC 3439384..3439950 (+) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  OLR76_RS16970 (OLR76_16880) lptC 3439947..3440522 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  OLR76_RS16975 (OLR76_16885) lptA 3440491..3441048 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  OLR76_RS16980 (OLR76_16890) lptB 3441055..3441780 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  OLR76_RS16985 (OLR76_16895) rpoN/rpoN1 3441828..3443261 (+) 1434 WP_282983251.1 RNA polymerase factor sigma-54 Machinery gene
  OLR76_RS16990 (OLR76_16900) hpf 3443284..3443571 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  OLR76_RS16995 (OLR76_16905) ptsN 3443689..3444180 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  OLR76_RS17000 (OLR76_16910) rapZ 3444226..3445080 (+) 855 WP_000243741.1 RNase adapter RapZ -
  OLR76_RS17005 (OLR76_16915) npr 3445077..3445349 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  OLR76_RS17010 (OLR76_16920) yrbL 3445563..3446195 (+) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  OLR76_RS17015 (OLR76_16925) mtgA 3446192..3446920 (-) 729 WP_061355135.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  OLR76_RS17020 (OLR76_16930) elbB 3446917..3447570 (-) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53973.74 Da        Isoelectric Point: 4.3663

>NTDB_id=682832 OLR76_RS16985 WP_282983251.1 3441828..3443261(+) (rpoN/rpoN1) [Escherichia coli strain HE_50]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
ILESIGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVEPNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=682832 OLR76_RS16985 WP_282983251.1 3441828..3443261(+) (rpoN/rpoN1) [Escherichia coli strain HE_50]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACCCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

43.825

100

0.461