Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PALA43_RS27895 Genome accession   NZ_CP109932
Coordinates   6010145..6011635 (+) Length   496 a.a.
NCBI ID   WP_269972399.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA43     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6005145..6016635
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA43_RS27865 (PALA43_05539) - 6005357..6006166 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  PALA43_RS27870 (PALA43_05541) kdsD 6006580..6007560 (+) 981 WP_003094347.1 arabinose-5-phosphate isomerase KdsD -
  PALA43_RS27875 (PALA43_05542) - 6007560..6008099 (+) 540 WP_003098849.1 KdsC family phosphatase -
  PALA43_RS27880 (PALA43_05543) lptC 6008108..6008680 (+) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  PALA43_RS27885 (PALA43_05544) lptA 6008667..6009194 (+) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  PALA43_RS27890 (PALA43_05545) lptB 6009194..6009919 (+) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  PALA43_RS27895 (PALA43_05546) rpoN/rpoN1 6010145..6011635 (+) 1491 WP_269972399.1 RNA polymerase factor sigma-54 Machinery gene
  PALA43_RS27900 (PALA43_05547) hpf 6011713..6012021 (+) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  PALA43_RS27905 (PALA43_05548) ptsN 6012035..6012499 (+) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PALA43_RS27910 (PALA43_05549) rapZ 6012501..6013361 (+) 861 WP_003094361.1 RNase adapter RapZ -
  PALA43_RS27915 (PALA43_05550) - 6013391..6013663 (+) 273 WP_003094362.1 HPr family phosphocarrier protein -
  PALA43_RS27920 (PALA43_05551) - 6013760..6014692 (-) 933 WP_003123303.1 ZIP family metal transporter -
  PALA43_RS27925 (PALA43_05552) - 6014711..6015322 (-) 612 WP_031643275.1 superoxide dismutase -
  PALA43_RS27930 (PALA43_05553) - 6015335..6015784 (-) 450 WP_003094369.1 hypothetical protein -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 55983.14 Da        Isoelectric Point: 4.4327

>NTDB_id=682116 PALA43_RS27895 WP_269972399.1 6010145..6011635(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain PALA43]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGRNLRECLLLQLRQLPSTTPWLNEALRLVS
DYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVNA
TYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHESTI
SRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAKY
RESLGIAPSSERKRLV

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=682116 PALA43_RS27895 WP_269972399.1 6010145..6011635(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain PALA43]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCTCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCTCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAATGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTACCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCCGCAACC
TGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTATCC
GACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCGCCA
GGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCCCCG
ACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCACGCCTGCGCGTCAACGCC
ACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCGCTG
GTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTTTCC
TCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCACGACATCGCCGAAGCGGTGGGCATGCACGAATCGACCATT
TCGCGGGTCACCACCCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGTCAG
TACCGCCGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGAAAA
AGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAATAC
CGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(336-494)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-322)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.457

100

0.476