Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   M0696_RS12795 Genome accession   NZ_CP096590
Coordinates   2536044..2536454 (-) Length   136 a.a.
NCBI ID   WP_084993633.1    Uniprot ID   -
Organism   Bacillus rugosus strain A78.1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2531044..2541454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0696_RS12765 (M0696_12765) - 2531060..2531200 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  M0696_RS12770 (M0696_12770) - 2531505..2532236 (-) 732 WP_222143200.1 SGNH/GDSL hydrolase family protein -
  M0696_RS12775 (M0696_12775) - 2532484..2533236 (-) 753 WP_222143201.1 N-acetylmuramoyl-L-alanine amidase -
  M0696_RS12780 (M0696_12780) - 2533427..2534053 (+) 627 WP_222143202.1 TVP38/TMEM64 family protein -
  M0696_RS12785 (M0696_12785) gnd 2534072..2534965 (-) 894 WP_222143203.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  M0696_RS12790 (M0696_12790) - 2535300..2536011 (+) 712 Protein_2476 DUF308 domain-containing protein -
  M0696_RS12795 (M0696_12795) nucA/comI 2536044..2536454 (-) 411 WP_084993633.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  M0696_RS12800 (M0696_12800) - 2536649..2536996 (+) 348 Protein_2478 sigma-70 family RNA polymerase sigma factor -
  M0696_RS12805 (M0696_12805) spoIVCA 2537027..2538487 (-) 1461 WP_248602773.1 site-specific DNA recombinase SpoIVCA -
  M0696_RS12810 (M0696_12810) - 2538445..2538623 (-) 179 Protein_2480 hypothetical protein -
  M0696_RS12815 (M0696_12815) - 2538884..2539756 (-) 873 WP_248602003.1 alpha/beta hydrolase -
  M0696_RS12820 (M0696_12820) - 2539945..2540688 (-) 744 WP_248602004.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14979.02 Da        Isoelectric Point: 5.2038

>NTDB_id=681970 M0696_RS12795 WP_084993633.1 2536044..2536454(-) (nucA/comI) [Bacillus rugosus strain A78.1]
MKKWMAGLLLAAAVFLCLVVPQPIKSASLYDKVLYFPLSRYPETGDHIRDAITEGHSDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSGYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=681970 M0696_RS12795 WP_084993633.1 2536044..2536454(-) (nucA/comI) [Bacillus rugosus strain A78.1]
ATGAAAAAATGGATGGCAGGCCTGCTTCTTGCTGCAGCAGTTTTTCTTTGTTTAGTGGTTCCGCAGCCGATCAAGAGCGC
TTCTTTGTATGACAAAGTGTTGTATTTTCCGCTGTCTCGTTATCCGGAAACAGGGGATCATATTAGAGATGCGATTACAG
AGGGACATTCTGATATTTGTACCATCGACAGGGATGGAGCGGACAAAAGGCGAGAGGAATCATTAAAAGGAATTCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCAATGGCGGTTTGCGAGGAAGGCGGCGCAGGAGCTGACGTCCGATATGTGAC
GCCTTCTGATAATCGCGGGGCCGGCTCGTGGGTAGGGAATCAAATGAGTGGTTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

63.478

84.559

0.537