Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M1B70_RS01825 Genome accession   NZ_CP096571
Coordinates   393699..395069 (+) Length   456 a.a.
NCBI ID   WP_002832361.1    Uniprot ID   A0AAN5Y9S9
Organism   Pediococcus acidilactici strain BB2-4M     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 388699..400069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1B70_RS01800 (M1B70_01800) - 389460..390131 (+) 672 WP_053906536.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  M1B70_RS01805 (M1B70_01805) - 390525..391871 (-) 1347 WP_002832366.1 C1 family peptidase -
  M1B70_RS01810 (M1B70_01810) rpiA 391920..392606 (-) 687 WP_002832365.1 ribose-5-phosphate isomerase RpiA -
  M1B70_RS01815 (M1B70_01815) - 392617..392940 (-) 324 WP_002832363.1 GNAT family N-acetyltransferase -
  M1B70_RS01820 (M1B70_01820) - 393069..393605 (+) 537 WP_005921602.1 dUTP diphosphatase dut -
  M1B70_RS01825 (M1B70_01825) radA 393699..395069 (+) 1371 WP_002832361.1 DNA repair protein RadA Machinery gene
  M1B70_RS01830 (M1B70_01830) - 395185..396333 (+) 1149 WP_002832360.1 PIN/TRAM domain-containing protein -
  M1B70_RS01835 (M1B70_01835) gltX 396458..397945 (+) 1488 WP_002832359.1 glutamate--tRNA ligase -
  M1B70_RS01840 (M1B70_01840) cysS 398102..399520 (+) 1419 WP_053906350.1 cysteine--tRNA ligase -
  M1B70_RS01845 (M1B70_01845) - 399513..399926 (+) 414 WP_002832357.1 Mini-ribonuclease 3 -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50252.79 Da        Isoelectric Point: 7.4153

>NTDB_id=681788 M1B70_RS01825 WP_002832361.1 393699..395069(+) (radA) [Pediococcus acidilactici strain BB2-4M]
MAKVKTQFVCNNCGYASPKFLGRCPNCGKWNTLVEERMSDPKAERKSRVSFDGKHTQPQLISDVAMHEEPRVKTGMEELN
RVLGGGVVDGSLVLIGGDPGIGKSTLLLQLSGQLAETQRKVLYVSGEESASQIKMRAERLKVNSERFYLYPETDMSSVRA
VIEELHPEYVIIDSVQTMQEPDIESAVGSVSQIREITAELMQIAKTNNITIFIVGHVTKGGAIAGPKILEHMVDTVLYFE
GDLHHTYRILRAVKNRFGSTNELGIFEMREEGLREVANPSEIFLEERLKDASGSAIVVSMEGTRPILVEIQALITPTIFG
NAQRTASGLDRNRVSLIMAVLEKRAKLTLQNQDAYLKAAGGVKLDEPAIDLALAISIVSSFKNQGTRPTDSFVGEIGLTG
EIRRVNRIEQRVAEAQKLGFKRILIPKNNLKGWQPPEGIEVVGVATIADAIRRAFN

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=681788 M1B70_RS01825 WP_002832361.1 393699..395069(+) (radA) [Pediococcus acidilactici strain BB2-4M]
ATGGCAAAAGTTAAGACCCAGTTTGTATGCAATAATTGCGGATACGCCTCCCCTAAATTTTTAGGACGCTGTCCCAATTG
TGGTAAGTGGAACACTTTAGTTGAAGAACGGATGAGCGACCCAAAGGCCGAACGAAAAAGTCGGGTGAGCTTTGATGGCA
AACATACGCAACCACAGTTGATTTCGGATGTTGCGATGCACGAGGAGCCCCGGGTAAAGACCGGGATGGAAGAACTTAAC
CGCGTGCTTGGTGGTGGGGTGGTTGACGGCTCCCTAGTCTTAATTGGTGGGGATCCTGGAATCGGAAAATCAACCCTGTT
GTTACAGCTATCAGGACAGTTGGCAGAGACGCAACGCAAAGTACTTTACGTTTCGGGTGAAGAAAGTGCTTCCCAGATTA
AGATGCGTGCCGAACGCTTGAAAGTTAATAGTGAGCGCTTCTATTTGTATCCAGAGACGGATATGTCTAGCGTGCGGGCG
GTGATTGAGGAGTTACACCCCGAATACGTGATCATTGATTCGGTTCAGACCATGCAGGAACCGGATATTGAATCGGCGGT
GGGGAGCGTTTCTCAAATTCGAGAAATTACCGCAGAATTGATGCAAATCGCGAAAACCAATAACATTACCATCTTCATCG
TTGGTCACGTGACCAAGGGCGGCGCAATTGCGGGCCCTAAGATTTTGGAACACATGGTGGATACGGTGCTTTACTTTGAA
GGTGATCTGCACCACACTTACCGGATCCTGCGGGCGGTGAAGAACCGCTTTGGTTCCACGAACGAATTAGGAATCTTTGA
GATGCGCGAAGAAGGGTTACGCGAGGTGGCTAACCCGTCAGAAATTTTCTTAGAAGAGCGTTTAAAAGATGCTTCGGGAT
CGGCAATTGTAGTTTCAATGGAAGGAACTCGGCCGATTTTAGTTGAAATACAAGCCTTGATCACGCCCACCATCTTCGGC
AATGCGCAGCGCACCGCGAGCGGGTTAGACCGCAACCGAGTTTCGCTGATCATGGCGGTGCTCGAAAAACGGGCAAAGCT
TACTTTGCAAAACCAAGACGCCTATTTAAAGGCGGCGGGCGGCGTCAAGCTTGACGAACCCGCAATTGACTTAGCGTTGG
CGATTAGTATTGTTTCAAGTTTTAAAAATCAGGGAACCCGGCCGACCGATAGTTTTGTTGGTGAAATCGGCTTAACTGGT
GAAATTCGGCGGGTTAACCGAATTGAACAACGGGTCGCTGAGGCACAAAAGCTTGGTTTTAAGCGGATTTTGATTCCGAA
GAATAATTTAAAGGGGTGGCAGCCCCCCGAAGGAATTGAAGTTGTAGGGGTAGCTACGATTGCCGACGCGATTCGGCGGG
CATTTAATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

67.033

99.781

0.669
  radA Streptococcus mitis NCTC 12261

66.813

99.781

0.667
  radA Streptococcus pneumoniae Rx1

66.813

99.781

0.667
  radA Streptococcus pneumoniae TIGR4

66.813

99.781

0.667
  radA Streptococcus pneumoniae D39

66.813

99.781

0.667
  radA Streptococcus pneumoniae R6

66.813

99.781

0.667
  radA Bacillus subtilis subsp. subtilis str. 168

65.351

100

0.654