Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   N8A72_RS02755 Genome accession   NZ_CP109924
Coordinates   574380..575813 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli O125ac:K+:H10 strain C 236-04A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 569380..580813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8A72_RS02720 (N8A72_02720) elbB 570071..570724 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  N8A72_RS02725 (N8A72_02725) mtgA 570721..571449 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  N8A72_RS02730 (N8A72_02730) yrbL 571446..572078 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  N8A72_RS02735 (N8A72_02735) npr 572292..572564 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  N8A72_RS02740 (N8A72_02740) rapZ 572561..573415 (-) 855 WP_000243741.1 RNase adapter RapZ -
  N8A72_RS02745 (N8A72_02745) ptsN 573461..573952 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  N8A72_RS02750 (N8A72_02750) hpf 574070..574357 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  N8A72_RS02755 (N8A72_02755) rpoN/rpoN1 574380..575813 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  N8A72_RS02760 (N8A72_02760) lptB 575861..576586 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  N8A72_RS02765 (N8A72_02765) lptA 576593..577150 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  N8A72_RS02770 (N8A72_02770) lptC 577119..577694 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  N8A72_RS02775 (N8A72_02775) kdsC 577691..578257 (-) 567 WP_000030011.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  N8A72_RS02780 (N8A72_02780) kdsD 578278..579264 (-) 987 WP_001325168.1 arabinose-5-phosphate isomerase KdsD -
  N8A72_RS02785 (N8A72_02785) yrbG 579278..580255 (-) 978 WP_000922872.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=681766 N8A72_RS02755 WP_000809051.1 574380..575813(-) (rpoN/rpoN1) [Escherichia coli O125ac:K+:H10 strain C 236-04A]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=681766 N8A72_RS02755 WP_000809051.1 574380..575813(-) (rpoN/rpoN1) [Escherichia coli O125ac:K+:H10 strain C 236-04A]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTTCAACAGGCGATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465