Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   M1F46_RS07970 Genome accession   NZ_CP096539
Coordinates   1645982..1646956 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain RIVM_M047065     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1640982..1651956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1F46_RS07930 (M1F46_07920) gcvT 1641483..1642574 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  M1F46_RS07935 (M1F46_07925) - 1642733..1643257 (-) 525 WP_001015120.1 shikimate kinase -
  M1F46_RS07940 (M1F46_07930) - 1643247..1643393 (-) 147 WP_001789879.1 hypothetical protein -
  M1F46_RS07945 (M1F46_07935) comGF 1643490..1643987 (-) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  M1F46_RS07950 (M1F46_07940) comGE 1643905..1644204 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  M1F46_RS07955 (M1F46_07945) comGD 1644191..1644637 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  M1F46_RS07960 (M1F46_07950) comGC 1644615..1644926 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  M1F46_RS07965 (M1F46_07955) comGB 1644940..1646010 (-) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  M1F46_RS07970 (M1F46_07960) comGA 1645982..1646956 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  M1F46_RS07975 (M1F46_07965) - 1647008..1647631 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  M1F46_RS07980 (M1F46_07970) - 1647628..1647957 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  M1F46_RS07985 (M1F46_07975) - 1647957..1648943 (-) 987 WP_000161315.1 ROK family glucokinase -
  M1F46_RS07990 (M1F46_07980) - 1648940..1649143 (-) 204 WP_000087559.1 YqgQ family protein -
  M1F46_RS07995 (M1F46_07985) - 1649124..1650587 (-) 1464 WP_001017856.1 rhomboid family intramembrane serine protease -
  M1F46_RS08000 (M1F46_07990) - 1650599..1651138 (-) 540 WP_000164554.1 5-formyltetrahydrofolate cyclo-ligase -
  M1F46_RS08005 (M1F46_07995) rpmG 1651347..1651496 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=681696 M1F46_RS07970 WP_000697220.1 1645982..1646956(-) (comGA) [Staphylococcus aureus strain RIVM_M047065]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=681696 M1F46_RS07970 WP_000697220.1 1645982..1646956(-) (comGA) [Staphylococcus aureus strain RIVM_M047065]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTACGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1