Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   M1F46_RS07945 Genome accession   NZ_CP096539
Coordinates   1643490..1643987 (-) Length   165 a.a.
NCBI ID   WP_001825340.1    Uniprot ID   A0A2S6DDC2
Organism   Staphylococcus aureus strain RIVM_M047065     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1638490..1648987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1F46_RS07920 (M1F46_07910) gcvPB 1638652..1640124 (-) 1473 WP_000202179.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  M1F46_RS07925 (M1F46_07915) gcvPA 1640117..1641463 (-) 1347 WP_000019698.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  M1F46_RS07930 (M1F46_07920) gcvT 1641483..1642574 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  M1F46_RS07935 (M1F46_07925) - 1642733..1643257 (-) 525 WP_001015120.1 shikimate kinase -
  M1F46_RS07940 (M1F46_07930) - 1643247..1643393 (-) 147 WP_001789879.1 hypothetical protein -
  M1F46_RS07945 (M1F46_07935) comGF 1643490..1643987 (-) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  M1F46_RS07950 (M1F46_07940) comGE 1643905..1644204 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  M1F46_RS07955 (M1F46_07945) comGD 1644191..1644637 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  M1F46_RS07960 (M1F46_07950) comGC 1644615..1644926 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  M1F46_RS07965 (M1F46_07955) comGB 1644940..1646010 (-) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  M1F46_RS07970 (M1F46_07960) comGA 1645982..1646956 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  M1F46_RS07975 (M1F46_07965) - 1647008..1647631 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  M1F46_RS07980 (M1F46_07970) - 1647628..1647957 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  M1F46_RS07985 (M1F46_07975) - 1647957..1648943 (-) 987 WP_000161315.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19179.92 Da        Isoelectric Point: 10.2192

>NTDB_id=681691 M1F46_RS07945 WP_001825340.1 1643490..1643987(-) (comGF) [Staphylococcus aureus strain RIVM_M047065]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=681691 M1F46_RS07945 WP_001825340.1 1643490..1643987(-) (comGF) [Staphylococcus aureus strain RIVM_M047065]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S6DDC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97