Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   M1F49_RS08290 Genome accession   NZ_CP096530
Coordinates   1681744..1682718 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain RIVM_M084526     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1676744..1687718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1F49_RS08250 (M1F49_08245) - 1677162..1677686 (-) 525 WP_001015120.1 shikimate kinase -
  M1F49_RS08255 (M1F49_08250) - 1677676..1677822 (-) 147 WP_001789879.1 hypothetical protein -
  M1F49_RS08260 (M1F49_08255) - 1677889..1679061 (-) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  M1F49_RS08265 (M1F49_08260) comGF 1679252..1679749 (-) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  M1F49_RS08270 (M1F49_08265) comGE 1679667..1679966 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  M1F49_RS08275 (M1F49_08270) comGD 1679953..1680399 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  M1F49_RS08280 (M1F49_08275) comGC 1680377..1680688 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  M1F49_RS08285 (M1F49_08280) comGB 1680702..1681772 (-) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  M1F49_RS08290 (M1F49_08285) comGA 1681744..1682718 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  M1F49_RS08295 (M1F49_08290) - 1682770..1683393 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  M1F49_RS08300 (M1F49_08295) - 1683390..1683719 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  M1F49_RS08305 (M1F49_08300) - 1683719..1684705 (-) 987 WP_000161315.1 ROK family glucokinase -
  M1F49_RS08310 (M1F49_08305) - 1684702..1684905 (-) 204 WP_000087559.1 YqgQ family protein -
  M1F49_RS08315 (M1F49_08310) - 1684886..1686349 (-) 1464 WP_001017856.1 rhomboid family intramembrane serine protease -
  M1F49_RS08320 (M1F49_08315) - 1686361..1686900 (-) 540 WP_000164554.1 5-formyltetrahydrofolate cyclo-ligase -
  M1F49_RS08325 (M1F49_08320) rpmG 1687109..1687258 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=681567 M1F49_RS08290 WP_000697220.1 1681744..1682718(-) (comGA) [Staphylococcus aureus strain RIVM_M084526]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=681567 M1F49_RS08290 WP_000697220.1 1681744..1682718(-) (comGA) [Staphylococcus aureus strain RIVM_M084526]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTACGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1