Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   ABRG53_RS00750 Genome accession   NZ_AP017560
Coordinates   176798..178810 (-) Length   670 a.a.
NCBI ID   WP_126384335.1    Uniprot ID   A0A2Z5WSV7
Organism   Pseudanabaena sp. ABRG5-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 171798..183810
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABRG53_RS00730 (ABRG53_0147) - 171868..173076 (+) 1209 WP_225886787.1 aldo/keto reductase -
  ABRG53_RS00735 (ABRG53_0148) - 173107..174114 (+) 1008 WP_126384330.1 aldo/keto reductase -
  ABRG53_RS00740 (ABRG53_0149) - 174165..175373 (-) 1209 WP_126384332.1 type II secretion system F family protein -
  ABRG53_RS00745 (ABRG53_0150) - 175437..176591 (-) 1155 WP_126389925.1 type IV pilus twitching motility protein PilT -
  ABRG53_RS00750 (ABRG53_0151) pilB/pilB1 176798..178810 (-) 2013 WP_126384335.1 GspE/PulE family protein Machinery gene
  ABRG53_RS00755 (ABRG53_0152) - 179221..181281 (-) 2061 WP_126384338.1 D-alanyl-lipoteichoic acid biosynthesis protein DltD -
  ABRG53_RS00760 (ABRG53_0153) - 181370..182356 (+) 987 WP_126384341.1 sensor domain-containing diguanylate cyclase -
  ABRG53_RS00765 (ABRG53_0154) - 182400..183236 (-) 837 WP_126384344.1 TPM domain-containing protein -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74784.38 Da        Isoelectric Point: 5.3372

>NTDB_id=67991 ABRG53_RS00750 WP_126384335.1 176798..178810(-) (pilB/pilB1) [Pseudanabaena sp. ABRG5-3]
MNTSDQAPNTAASRNRTKALAKRGGRTPFESKLIQEGHATSDQFDRAVKDSQEQNVPFLMALEQILGQQLAPDITRYYKR
QQLFELRVLYGIEPIDPDVEVDKFDIPTLSTLLDSNIIPVSSCRDCEILPLSKSENSITLAVVAPDSYKVQNEIARLLKN
ITVIRRVIAREDFHRTFDNIVQFQINKSSKAEGAISDEDLQVKDDVFGEDITDADQGDELMVGGENSAPIISLVDKILVR
ALKEGCSDIHIEPQEKDLCIRLRKDGVLREYFFLSDNKRLPKNIINAVISRFKIISNLNIAEKRVPQDGKLKRNFQGRRV
DFRVNTCPTQNGEKVCLRILDNSNTQLGLNKLISDPESLELMQSFAKRPYGLILVTGPTGSGKTTTLYSTLAECNDPGIN
ISTAEDPVEYNLPGLTQCQVLREKGMTFASILRAFLRQDPDVILVGETRDAETAKTAIEAALTGHLVLTTLHTNDAPSCI
ARLEEMGVEPFMASTAIIGVLAQRLLRRVCDNCRIPYNPSQEELDRFGLPSTDLEKTFYRANIVNREAMLPGQRVCPKCN
GSGYKGRAGVYEIMKMTERLQSAINKGATTEVIKEIAVQEGMKTLMAYAFDLVRQGATTLDEVLRVVYTDKGREAEERAR
RGKGLECDNCDAMLTPETVECPYCTTPRTF

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=67991 ABRG53_RS00750 WP_126384335.1 176798..178810(-) (pilB/pilB1) [Pseudanabaena sp. ABRG5-3]
ATGAATACATCAGATCAAGCGCCAAATACTGCCGCGAGCAGAAACAGAACCAAAGCTCTTGCTAAACGGGGAGGTCGCAC
TCCTTTTGAGAGCAAACTCATTCAAGAAGGACATGCTACCTCAGACCAGTTCGATCGCGCTGTCAAGGATAGTCAAGAAC
AGAATGTTCCATTTCTCATGGCCCTAGAGCAAATTTTGGGTCAACAACTTGCCCCAGACATTACCCGCTATTACAAGCGC
CAACAGCTTTTTGAACTGCGTGTCCTTTACGGCATCGAACCGATCGACCCCGATGTTGAAGTTGACAAATTTGATATCCC
CACCCTCAGTACCTTACTCGACTCCAATATTATCCCCGTTTCTAGTTGCCGCGACTGTGAGATTCTCCCTCTATCCAAAA
GTGAAAACAGCATTACCCTTGCAGTAGTTGCGCCCGACAGTTATAAAGTCCAAAATGAAATTGCACGACTGCTCAAAAAT
ATAACGGTGATTAGGCGAGTGATTGCTCGCGAGGATTTTCATCGCACATTTGATAATATTGTGCAATTTCAAATCAACAA
GAGTTCCAAGGCTGAAGGCGCAATCAGTGACGAAGATCTCCAAGTTAAAGATGATGTCTTTGGTGAAGATATAACAGATG
CAGATCAAGGCGATGAACTCATGGTTGGGGGCGAAAACTCCGCCCCAATCATTAGCCTTGTAGACAAAATCTTAGTCAGA
GCGCTCAAAGAAGGATGCTCAGATATTCACATCGAGCCACAGGAAAAAGATCTCTGCATTCGTCTGCGTAAGGATGGTGT
ATTGCGAGAATACTTCTTCCTCAGTGACAACAAGCGTTTACCCAAGAACATTATCAACGCTGTTATTTCTCGCTTCAAGA
TTATTTCTAACCTCAATATCGCGGAGAAACGGGTTCCCCAAGATGGCAAGCTGAAGCGTAACTTCCAAGGTCGTCGTGTA
GATTTCCGTGTCAATACCTGCCCCACCCAAAACGGTGAAAAAGTATGTCTACGGATTCTCGACAACTCCAATACCCAACT
TGGACTGAATAAACTGATCAGCGATCCCGAAAGTTTGGAACTGATGCAAAGTTTTGCCAAACGTCCCTATGGACTGATTC
TAGTTACAGGTCCTACGGGGTCAGGTAAAACCACCACTCTCTACTCAACCCTCGCTGAATGTAACGATCCAGGCATTAAC
ATTAGTACTGCCGAAGATCCCGTTGAATATAATCTTCCTGGACTGACTCAATGCCAAGTTCTACGGGAAAAAGGCATGAC
CTTTGCCAGTATTCTCCGCGCTTTCCTCCGCCAAGATCCTGACGTAATCCTTGTGGGTGAGACTCGCGACGCAGAAACTG
CCAAAACCGCGATCGAAGCGGCGCTAACAGGTCACTTAGTCTTAACAACACTACATACCAACGATGCACCTAGTTGTATT
GCCCGTCTTGAAGAAATGGGTGTCGAACCATTCATGGCAAGTACAGCGATTATTGGTGTGCTAGCTCAGCGTCTACTCCG
CCGAGTCTGTGACAACTGCCGCATCCCCTACAATCCATCGCAAGAGGAACTCGATCGCTTTGGCTTGCCCAGTACTGACC
TCGAAAAAACCTTCTACCGAGCTAATATCGTTAACCGTGAAGCCATGCTCCCCGGTCAGCGCGTATGTCCAAAATGTAAC
GGCTCTGGATACAAGGGTCGGGCAGGTGTCTACGAGATCATGAAAATGACCGAACGTCTGCAAAGCGCGATCAACAAGGG
GGCAACCACTGAAGTGATCAAAGAAATTGCTGTCCAAGAAGGGATGAAAACCTTGATGGCATATGCCTTTGATTTGGTCA
GACAGGGCGCAACTACTCTTGATGAAGTACTACGTGTGGTTTACACAGACAAAGGAAGAGAAGCCGAAGAACGCGCTCGT
CGAGGCAAAGGGCTTGAATGCGACAACTGTGATGCTATGCTCACACCTGAAACTGTCGAATGTCCTTACTGCACCACACC
GAGAACCTTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(71-180)

Type II/IV secretion system protein (T2SSE)

(236-508)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z5WSV7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

51.791

100

0.518

Multiple sequence alignment