Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MZO21_RS02455 Genome accession   NZ_CP096120
Coordinates   474357..475121 (+) Length   254 a.a.
NCBI ID   WP_248101600.1    Uniprot ID   -
Organism   Acinetobacter portensis strain JNE5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 469357..480121
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MZO21_RS02425 (MZO21_02420) - 469496..470119 (+) 624 WP_154772145.1 DUF2971 domain-containing protein -
  MZO21_RS02430 (MZO21_02425) - 470204..471478 (+) 1275 WP_154772099.1 glycosyltransferase family 1 protein -
  MZO21_RS02435 (MZO21_02430) - 471511..472062 (+) 552 WP_154772100.1 phosphatase PAP2 family protein -
  MZO21_RS02440 (MZO21_02435) iscX 472204..472404 (+) 201 WP_154772101.1 Fe-S cluster assembly protein IscX -
  MZO21_RS02445 (MZO21_02440) ndk 472511..472942 (+) 432 WP_120401584.1 nucleoside-diphosphate kinase -
  MZO21_RS02450 (MZO21_02445) rlmN 473067..474299 (+) 1233 WP_248101568.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  MZO21_RS02455 (MZO21_02450) pilF 474357..475121 (+) 765 WP_248101600.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  MZO21_RS02460 (MZO21_02455) - 475112..475906 (+) 795 WP_154772103.1 helix-turn-helix domain-containing protein -
  MZO21_RS02465 (MZO21_02460) ispG 475930..477051 (+) 1122 WP_154772104.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  MZO21_RS02470 (MZO21_02465) hisS 477055..478350 (+) 1296 WP_154772105.1 histidine--tRNA ligase -
  MZO21_RS02475 (MZO21_02470) - 478381..479082 (+) 702 WP_248101565.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 28743.50 Da        Isoelectric Point: 9.2066

>NTDB_id=679563 MZO21_RS02455 WP_248101600.1 474357..475121(+) (pilF) [Acinetobacter portensis strain JNE5]
MVGATFLVVGCQTVSDRKVDPEKAVQARTQLAAEYIRTGDFDAAKRELDQAMQVNPRNALVNMMMGVLLQREGSQLNMEK
ADRYFKAAISADPKNAQARNNYGTYLYQAGRYNEALTHLEIAGATLGYDQRYTAFESAGRIYLKLNDVNKAENSFNQALK
ISRNSYISMVELAEIYYLRQDIPAATRLYENSVSLAGQNNQNARALWVGIRLARANGNPMQTQVLVNQLRALYPDSPEYQ
RYLQLQYSTEVVWK

Nucleotide


Download         Length: 765 bp        

>NTDB_id=679563 MZO21_RS02455 WP_248101600.1 474357..475121(+) (pilF) [Acinetobacter portensis strain JNE5]
ATTGTGGGTGCTACTTTTTTAGTTGTGGGTTGTCAGACAGTATCTGATCGCAAGGTTGATCCTGAAAAAGCAGTTCAAGC
AAGAACGCAGTTGGCTGCTGAATATATTCGAACTGGCGACTTTGATGCAGCAAAACGTGAATTAGACCAAGCCATGCAGG
TTAATCCACGTAATGCACTTGTAAATATGATGATGGGTGTGTTGTTGCAACGGGAAGGTAGTCAGCTCAATATGGAAAAA
GCTGATCGCTACTTTAAAGCAGCCATTTCAGCAGATCCTAAAAATGCACAAGCTCGTAACAACTATGGTACTTATTTGTA
TCAAGCTGGTCGTTATAATGAAGCATTAACGCACTTAGAGATTGCAGGTGCAACATTAGGTTATGATCAGCGTTATACTG
CATTTGAAAGTGCGGGACGTATTTATTTAAAGCTAAATGATGTTAATAAGGCTGAAAATAGCTTTAATCAAGCTTTAAAA
ATTAGCCGTAACTCATATATCTCAATGGTCGAATTGGCTGAGATATATTATTTAAGACAAGATATTCCTGCTGCGACTAG
ACTTTATGAAAATTCTGTCAGTTTAGCTGGACAAAATAATCAAAATGCACGTGCTTTGTGGGTGGGTATTCGACTTGCAC
GCGCAAATGGTAATCCTATGCAAACACAGGTTCTTGTGAATCAATTACGTGCTTTGTATCCGGATAGTCCAGAATACCAA
CGCTATTTGCAATTACAGTACAGTACTGAGGTCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(26-57)

Tetratricopeptide repeat (TPR_8)

(138-164)

Tetratricopeptide repeat (TPR_17)

(85-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

67.589

99.606

0.673
  pilF Acinetobacter baylyi ADP1

64.567

100

0.646