Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   MU858_RS21865 Genome accession   NZ_CP095874
Coordinates   4194236..4194982 (-) Length   248 a.a.
NCBI ID   WP_000487010.1    Uniprot ID   Q81LT9
Organism   Bacillus sp. PGP15     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4189236..4199982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MU858_RS21850 (MU858_21850) dnaG 4190003..4191799 (-) 1797 WP_000528216.1 DNA primase -
  MU858_RS21855 (MU858_21855) - 4192654..4193466 (-) 813 WP_000368943.1 pyruvate, water dikinase regulatory protein -
  MU858_RS21860 (MU858_21860) - 4193496..4194128 (-) 633 WP_000583758.1 helix-turn-helix transcriptional regulator -
  MU858_RS21865 (MU858_21865) recO 4194236..4194982 (-) 747 WP_000487010.1 DNA repair protein RecO Machinery gene
  MU858_RS21870 (MU858_21870) - 4195016..4195159 (-) 144 WP_000883924.1 YqzL family protein -
  MU858_RS21875 (MU858_21875) era 4195336..4196241 (-) 906 WP_048531222.1 GTPase Era -
  MU858_RS21880 (MU858_21880) - 4196234..4196632 (-) 399 WP_001086293.1 cytidine deaminase -
  MU858_RS21885 (MU858_21885) - 4196732..4197085 (-) 354 WP_166688038.1 diacylglycerol kinase family protein -
  MU858_RS21890 (MU858_21890) ybeY 4197082..4197552 (-) 471 WP_048531226.1 rRNA maturation RNase YbeY -
  MU858_RS21895 (MU858_21895) - 4197549..4199693 (-) 2145 WP_048531228.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28665.22 Da        Isoelectric Point: 8.0540

>NTDB_id=679015 MU858_RS21865 WP_000487010.1 4194236..4194982(-) (recO) [Bacillus sp. PGP15]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGAKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDQYRIPVGEAVHKLLRLFYHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=679015 MU858_RS21865 WP_000487010.1 4194236..4194982(-) (recO) [Bacillus sp. PGP15]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTCTCAAG
AGAATTGGGTAAGGTAAGTGCGATGGCAAGAGGAGCGAAAAAACCGAAAAGCCGGTTAGCATCTGTTTCGCAACTTATGA
CACATGGTCATTTCCTTATTCAAATGGGATCTGGACTTGGAACTTTGCAACAAGGCGAAATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAGTTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCATATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGACCCAGAGGTATTATCATTGA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGATACATGTGCGATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTCCGGGAAGGCGGTTTTCTGTGCTCTCGTCATGCTGAACAAGATCAGTATCGTATACCGGTGGG
GGAAGCTGTTCATAAATTATTGCGTCTTTTTTACCACTTCGATCTACATAGACTTGGCAATGTATCAGTGAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGGATTTATTTGAAATCAAGACGTTTCTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q81LT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552