Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MU858_RS00530 Genome accession   NZ_CP095874
Coordinates   97555..98931 (+) Length   458 a.a.
NCBI ID   WP_001085207.1    Uniprot ID   A0A2B4FB19
Organism   Bacillus sp. PGP15     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92555..103931
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MU858_RS00510 (MU858_00510) ctsR 92748..93209 (+) 462 WP_048526354.1 transcriptional regulator CtsR -
  MU858_RS00515 (MU858_00515) - 93383..93931 (+) 549 WP_048526355.1 UvrB/UvrC motif-containing protein -
  MU858_RS00520 (MU858_00520) - 93936..95000 (+) 1065 WP_048526356.1 protein arginine kinase -
  MU858_RS00525 (MU858_00525) clpC 95023..97458 (+) 2436 WP_000971179.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  MU858_RS00530 (MU858_00530) radA 97555..98931 (+) 1377 WP_001085207.1 DNA repair protein RadA Machinery gene
  MU858_RS00535 (MU858_00535) disA 98935..100008 (+) 1074 WP_048526357.1 DNA integrity scanning diadenylate cyclase DisA -
  MU858_RS00540 (MU858_00540) - 100169..101278 (+) 1110 WP_048526358.1 PIN/TRAM domain-containing protein -
  MU858_RS00545 (MU858_00545) ispD 101295..101975 (+) 681 WP_048526359.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MU858_RS00550 (MU858_00550) ispF 102091..102567 (+) 477 WP_166689495.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49814.39 Da        Isoelectric Point: 7.1421

>NTDB_id=678984 MU858_RS00530 WP_001085207.1 97555..98931(+) (radA) [Bacillus sp. PGP15]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
AHIEEVNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=678984 MU858_RS00530 WP_001085207.1 97555..98931(+) (radA) [Bacillus sp. PGP15]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGGTATCAGTCACCAAAATATATGGGGAAATGCCCTGGGTG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCAGTTGTATCATCAAGGCGCCTTAATTATGCCAATGCGATTCAAT
CAGAAGTAACAAAACCAAGACGTCTAACAGAAGTAGAAACAAAGTCTGAGGCGCGTATTGAAACGAAATTTCAAGAATTT
AACCGCGTACTTGGTGGCGGGATTGTAGATGGGTCCTTAGTACTTATTGGTGGTGACCCTGGGATTGGAAAATCAACGTT
ACTATTACAAATATCATCGCAATTAGCAGATTCTTCATATGATGTACTATATATATCGGGTGAAGAATCAGCAAAACAGA
TTAAACTGCGTGCGGACCGTTTACATGTAAATGGTAGCAATCTATTTGTTGTATCAGAGACGGATTTGCAGCGGATTGCA
GCACACATTGAAGAAGTGAATCCAGCCTTTGTTGTTATTGATTCTATCCAAACGATACATTTACCTGAGGTAACGTCAGC
ACCAGGAAGTGTGGCGCAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACGAAAGGGATCCCTATTTTTA
TCGTTGGACATGTGACAAAAGAGGGGGCAATTGCAGGGCCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTATTTT
GAAGGAGACCGTCATCATACATATCGTATTTTGCGAGCGGTGAAGAATCGTTTTGGTTCCACGAATGAAATGGGTATTTT
TGAAATGAAAGAACTTGGCCTTGCGGAAGTATTAAATCCTTCTGAAATTTTCCTTGAGGAAAGACCAGTTGGGGTTGCAG
GGTCAACGGTGGTTGCCTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCAACGGGAATTGACCATAACCGTGTATCGCTTATTATGGCAGTGTTAGAAAAAAGAACAGG
TTTATTATTGCAAAACCAAGACGCATATTTAAAAGTAGCAGGTGGTCTGAAGTTAGATGAACCAGCAATTGATTTAGCTG
TGGCTTTAAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCACCAACCGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTGTCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGCGCTATTATTCC
TAGAAAAAATTTAGGGGGATGGACAATTCCTGATGGGATTGAAGTAGTAGGTGTATCCAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B4FB19

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.728

100

0.749
  radA Streptococcus pneumoniae Rx1

63.797

98.908

0.631
  radA Streptococcus pneumoniae D39

63.797

98.908

0.631
  radA Streptococcus pneumoniae R6

63.797

98.908

0.631
  radA Streptococcus pneumoniae TIGR4

63.797

98.908

0.631
  radA Streptococcus mitis SK321

63.797

98.908

0.631
  radA Streptococcus mitis NCTC 12261

63.576

98.908

0.629