Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   MYE78_RS12150 Genome accession   NZ_CP095842
Coordinates   2518608..2519222 (-) Length   204 a.a.
NCBI ID   WP_032873955.1    Uniprot ID   -
Organism   Bacillus velezensis strain EM-1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2513608..2524222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MYE78_RS12120 rpsT 2513911..2514177 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  MYE78_RS12125 holA 2514194..2515235 (-) 1042 Protein_2343 DNA polymerase III subunit delta -
  MYE78_RS12130 - 2515275..2515426 (-) 152 Protein_2344 hypothetical protein -
  MYE78_RS12135 - 2515467..2515601 (+) 135 WP_003152870.1 YqzM family protein -
  MYE78_RS12140 comEC 2515620..2517971 (-) 2352 WP_032873958.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  MYE78_RS12145 - 2517972..2518541 (-) 570 WP_003152868.1 ComE operon protein 2 -
  MYE78_RS12150 comEA 2518608..2519222 (-) 615 WP_032873955.1 helix-hairpin-helix domain-containing protein Machinery gene
  MYE78_RS12155 comER 2519281..2520102 (+) 822 WP_012118027.1 late competence protein ComER -
  MYE78_RS12160 - 2520171..2520908 (-) 738 WP_032873951.1 class I SAM-dependent methyltransferase -
  MYE78_RS12165 rsfS 2520905..2521261 (-) 357 WP_007408260.1 ribosome silencing factor -
  MYE78_RS12170 yqeK 2521265..2521840 (-) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  MYE78_RS12175 - 2521830..2522399 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  MYE78_RS12180 yhbY 2522410..2522700 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  MYE78_RS12185 aroE 2522694..2523536 (-) 843 WP_032873946.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22265.47 Da        Isoelectric Point: 6.4940

>NTDB_id=678759 MYE78_RS12150 WP_032873955.1 2518608..2519222(-) (comEA) [Bacillus velezensis strain EM-1]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=678759 MYE78_RS12150 WP_032873955.1 2518608..2519222(-) (comEA) [Bacillus velezensis strain EM-1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363