Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MYF86_RS02615 Genome accession   NZ_CP095841
Coordinates   543438..544877 (-) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas veronii strain AV040     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 538438..549877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MYF86_RS02585 (MYF86_02585) - 538706..539446 (+) 741 WP_247823587.1 KDGP aldolase family protein -
  MYF86_RS02590 (MYF86_02590) - 539554..541458 (+) 1905 WP_247823588.1 PRD domain-containing protein -
  MYF86_RS02595 (MYF86_02595) npr 541476..541748 (-) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  MYF86_RS02600 (MYF86_02600) rapZ 541769..542635 (-) 867 WP_247823589.1 RNase adapter RapZ -
  MYF86_RS02605 (MYF86_02605) ptsN 542681..543127 (-) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  MYF86_RS02610 (MYF86_02610) hpf 543130..543417 (-) 288 WP_005336323.1 ribosome hibernation promoting factor -
  MYF86_RS02615 (MYF86_02615) rpoN/rpoN1 543438..544877 (-) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  MYF86_RS02620 (MYF86_02620) lptB 544943..545668 (-) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  MYF86_RS02625 (MYF86_02625) lptA 545672..546199 (-) 528 WP_040068817.1 lipopolysaccharide transport periplasmic protein LptA -
  MYF86_RS02630 (MYF86_02630) lptC 546186..546746 (-) 561 WP_005336338.1 LPS export ABC transporter periplasmic protein LptC -
  MYF86_RS02635 (MYF86_02635) kdsC 546743..547297 (-) 555 WP_101530526.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  MYF86_RS02640 (MYF86_02640) - 547297..548292 (-) 996 WP_058057518.1 KpsF/GutQ family sugar-phosphate isomerase -
  MYF86_RS02645 (MYF86_02645) mlaF 548500..549300 (+) 801 WP_005336343.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=678686 MYF86_RS02615 WP_005336331.1 543438..544877(-) (rpoN/rpoN1) [Aeromonas veronii strain AV040]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=678686 MYF86_RS02615 WP_005336331.1 543438..544877(-) (rpoN/rpoN1) [Aeromonas veronii strain AV040]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGCTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACCAGTCCGGAAGCGCCGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCCCAGGAGCAGATGCCG
GACGAGTTGCCGGTGGATACCACCTGGGACGAGGTTTACTCTGCCGGCACAGCCCAGAGCGCGGGCCCCATCCATGATGG
GGAAGATAGCGTCTATCAGGGTGAAACCACCGAAAACCTGCAGGATTATCTGCTGTGGCAGATGCGGCTCACACCGTTTA
GCGATGTGGATGCCGCCATCGCGCTCGCCATCATCGATGCCATCGACGAATCCGGTTATCTGACCGTTGCGCTGGAGGAT
ATCCAGGCTGCCGTCACCAGCGATGATGTAGAGGTGGAACTCGATGAGGTCGAGGCGGTACTCAAGCGGGTGCAGCACTT
CGATCCGGTCGGTATTGCTGCCCGTTCTGTGCAGGAGTGTTTGCTCATCCAGCTGGATCAGTATGACCCGCAGACCCCCT
GGCTCAACGAGGCCAAAGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGCACCCTCGCCCGCAAG
ACAAAACTCAAGGAGAGCGACCTCAAGGAGGTGCTGGATCTGATCCAGCTGCTGGAGCCGCGCCCCGGCAACGGCATTAT
CCAGAGCGACTCCCAGTACGTGATCCCCGACGTCTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCTGCCCCCCGCATTCGGGTCAATGAGACCTATGCCGCCATGGCCCGCAACGCCCGCAGCAGCACCGACACCCAGTTT
ATCCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAATGACATCGCCG
AAGCGGTAGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCCCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6N7F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491