Detailed information NTG0678286

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: MYW48_RS05540
• Genome accession: NZ_CP095767
• Coordinates: 1098242..1101967 (+)
• Length: 1241 a.a.
• NCBI ID: WP_106081478.1
• Uniprot ID: -
• Organism: Bacillus cereus strain CDHWZ7
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1093242..1106967

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MYW48_RS05525 (MYW48_05525)	-	1093401..1093994 (+)	594	WP_000347517.1	TVP38/TMEM64 family protein	-
MYW48_RS05530 (MYW48_05530)	lepB	1094051..1094614 (+)	564	WP_000751896.1	signal peptidase I	-
MYW48_RS05535 (MYW48_05535)	addB	1094730..1098245 (+)	3516	WP_106081479.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
MYW48_RS05540 (MYW48_05540)	addA	1098242..1101967 (+)	3726	WP_106081478.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
MYW48_RS05545 (MYW48_05545)	-	1101980..1102267 (+)	288	WP_106081477.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
MYW48_RS05550 (MYW48_05550)	gerPF	1102405..1102620 (-)	216	WP_001141566.1	spore germination protein GerPF	-
MYW48_RS05555 (MYW48_05555)	-	1102663..1103049 (-)	387	WP_000902327.1	spore germination protein GerPE	-
MYW48_RS05560 (MYW48_05560)	gerPD	1103065..1103259 (-)	195	WP_001052807.1	spore germination protein GerPD	-
MYW48_RS05565 (MYW48_05565)	gerPC	1103266..1103880 (-)	615	WP_001070761.1	spore germination protein GerPC	-
MYW48_RS05570 (MYW48_05570)	gerPB	1103948..1104154 (-)	207	WP_001012509.1	spore germination protein GerPB	-
MYW48_RS05575 (MYW48_05575)	gerPA	1104169..1104390 (-)	222	WP_001111188.1	spore germination protein GerPA	-
MYW48_RS05580 (MYW48_05580)	-	1104487..1104666 (-)	180	WP_000462848.1	aspartyl-phosphate phosphatase Spo0E family protein	-
MYW48_RS05585 (MYW48_05585)	-	1104913..1105812 (+)	900	WP_001213078.1	fumarylacetoacetate hydrolase family protein	-
MYW48_RS05590 (MYW48_05590)	-	1105848..1106150 (-)	303	WP_106081476.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142743.96 Da
• Isoelectric Point: 4.8307

>NTDB_id=678286 MYW48_RS05540 WP_106081478.1 1098242..1101967(+) (addA) [Bacillus cereus strain CDHWZ7]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADVALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDAGAELKLGATYPEGEEVAAELLCIQQTEEEVIDGEEGTEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEDLATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHEKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTNWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKKEVYDRLMWKYGYEDATSYRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLTAEEAYQDWQGKSEETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHGVNIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=678286 MYW48_RS05540 WP_106081478.1 1098242..1101967(+) (addA) [Bacillus cereus strain CDHWZ7]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCTGTTGTAGCGAACGGACG
TGATATTTTAGTTGCGGCTGCAGCTGGATCAGGTAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAGAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACAAATGCAGCAGCGCAAGAGATGAAAAATCGAATT
GGGGAAGCGTTAGAAAAGGTATTAATTGATGAACCAGGCTCTCAGCATGTAAGAAAGCAACTGAGCCTATTAAATAAAGC
TTCCATTTCAACAATTCACTCATTCTGTTTACAAGTAATTAGAGGATATTACTACATGCTGGATGTTGATCCTCGTTTCC
GTATTGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTACGGAATCGAA
GATAATACAATCTTCTTTGAACTTGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAACGTATGATTTTAGC
GCTTCATACAGAGTCAAGAGCGCATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACAATTGAAGATTTAGTATATGCTTCTTATTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAGGCAACTGAACTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACCCTGCAAGCAGATGTAGCATTACT
TGGAACGTTATCATCAGCTGCTCGTGAGTCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTA
AAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAATCCGTCAGCAGTGGCGCTCCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTTGTACAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAGCAGTCGATTTATCGTTTCCGACTAGCTG
AACCAGGACTATTCCTAGGAAAGTATAAACGCTTCACACAAGAAGGATTGGGCGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTACTAGCAGGTACGAACTTCATTTTCAAACAAATTATGGGCGAAGAAGTTGGAGAGAT
TGACTACGATGCTGGCGCTGAATTAAAACTAGGTGCTACCTATCCAGAAGGTGAAGAGGTAGCGGCAGAATTACTCTGTA
TTCAGCAAACGGAAGAAGAAGTAATAGACGGTGAAGAAGGTACAGAAGTCGAAAAAGCACAGCTTGAAGCTCGCCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCCGGTTATGAAGTGTATGACCGTAAAACGGATAGTATGCGTCCTGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCGG
TATATGCTGACCTTGCGACTGGTTATTTTGAAGCGACGGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATCCCGCTTGCAGCTGTACTTCGTTCCCCAATCGTTGGATTAAACGATGAAGACCTTGCGACGCTTCG
TGCTCACGGAAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGTGCACCGCTTGAAGAGGAACAAGAAC
TTCATGAAAAACTAGAGTGGTTTTATAACTTACTGCAAGGATGGCGTGAATTTGCACGTCAACAATCACTTTCTGATTTA
ATTTGGAAAGTGTACGGCGAGACAGGTTATTACGACTTCGTTGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
ACGCGTACTATATGACCGTGCAAGACAATATGAAGCAACATCATTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGATGATATGGGGACGGCGAGGGCCCTCGGTGAACAAGAAGACGTTGTTCGCATCATGACGATC
CATAAAAGTAAAGGGCTAGAGTTCCCGGTTGTATTTGTAGCTGGACTCGGCCGTCGTTTTAATACACAAGACTTAATGAA
ACGTTTCTTACTCCATAAAGATTTCGGTTTCGGTTCGCAATTTATCGATCCGCGTAAACGAATTAAATACACGACATTAT
CACAGCTTGCGATTAAACGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCGTTGACGCGT
GCGAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGGGAACA
TAGTGAATGGTTATTACCGGATCACATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGTATTCCAGATGAAATTTACGGGTATGACACGAACTGGAAA
GTAGAAGTTGTGGACGGTAACACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCGCTTCG
TGAGAAAAAGGCTGTTCCCTTGCAAAGTGAACGGAAAAAAGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
ATGCAACATCTTATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAACTACGTGCGCCAATTAAAACACGTCCGCGTTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGTGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGGTTGAAGTTCTGCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GATTTAGGTAAAAGGGTACTAGCGGCGAAAAGTGTTGAGCGTGAAGTGCCATTTACGATGATGCTTACAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGTGAAGAAACGATTCTTGTCCAAGGGGTTATCGACTGTATGATTGAAGAGGAAGACG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTCCCAGGCGGATTCGAACAAGCAAAACCAATTTTA
GAAGATCGATATAAAGTACAGCTTTCGTTATATGCAAAAGCGCTGGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGGAATCATGGTGTAAATATTGAGGAATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.339	100	0.534