Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   JLF30_RS02590 Genome accession   NZ_AP022478
Coordinates   553746..555179 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain STN0717-11     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 548746..560179
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JLF30_RS02555 (STN0717ESC11_05050) elbB 549438..550091 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  JLF30_RS02560 (STN0717ESC11_05060) mtgA 550088..550816 (+) 729 WP_000047064.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  JLF30_RS02565 (STN0717ESC11_05070) yrbL 550813..551445 (-) 633 WP_000620396.1 PhoP regulatory network protein YrbL -
  JLF30_RS02570 (STN0717ESC11_05080) npr 551658..551930 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  JLF30_RS02575 (STN0717ESC11_05090) rapZ 551927..552781 (-) 855 WP_000243741.1 RNase adapter RapZ -
  JLF30_RS02580 (STN0717ESC11_05100) ptsN 552827..553318 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  JLF30_RS02585 (STN0717ESC11_05110) hpf 553436..553723 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  JLF30_RS02590 (STN0717ESC11_05120) rpoN/rpoN1 553746..555179 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  JLF30_RS02595 (STN0717ESC11_05130) lptB 555227..555952 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  JLF30_RS02600 (STN0717ESC11_05140) lptA 555959..556516 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  JLF30_RS02605 (STN0717ESC11_05150) lptC 556485..557060 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  JLF30_RS02610 (STN0717ESC11_05160) kdsC 557057..557623 (-) 567 WP_000030006.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  JLF30_RS02615 (STN0717ESC11_05170) kdsD 557644..558630 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  JLF30_RS02620 (STN0717ESC11_05180) yrbG 558644..559621 (-) 978 WP_000922873.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=67819 JLF30_RS02590 WP_000809051.1 553746..555179(-) (rpoN/rpoN1) [Escherichia coli strain STN0717-11]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=67819 JLF30_RS02590 WP_000809051.1 553746..555179(-) (rpoN/rpoN1) [Escherichia coli strain STN0717-11]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGATACTGACCGCGCCATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGTTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATTATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACGGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCATTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465

Multiple sequence alignment