Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   MX663_RS20145 Genome accession   NZ_CP095736
Coordinates   3847274..3848419 (+) Length   381 a.a.
NCBI ID   WP_160245473.1    Uniprot ID   -
Organism   Bacillus subtilis strain N4     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3842274..3853419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MX663_RS20125 (MX663_20125) albF 3842290..3843570 (+) 1281 WP_247470914.1 pitrilysin family protein -
  MX663_RS20130 (MX663_20130) albG 3843567..3844268 (+) 702 WP_015250988.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  MX663_RS20135 (MX663_20135) ywhL 3844274..3845650 (-) 1377 WP_015250987.1 YncE family protein -
  MX663_RS20140 (MX663_20140) ywhK 3845689..3847044 (-) 1356 WP_015250986.1 YncE family protein -
  MX663_RS22100 - 3847041..3847271 (-) 231 Protein_3920 hypothetical protein -
  MX663_RS20145 (MX663_20145) rapF 3847274..3848419 (+) 1146 WP_160245473.1 response regulator aspartate phosphatase RapF Regulator
  MX663_RS20150 (MX663_20150) phrF 3848403..3848522 (+) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  MX663_RS20155 (MX663_20155) - 3848775..3849254 (+) 480 WP_015714892.1 hypothetical protein -
  MX663_RS20160 (MX663_20160) - 3849403..3850191 (+) 789 WP_015714893.1 membrane protein -
  MX663_RS20165 (MX663_20165) - 3850178..3850852 (+) 675 WP_015714894.1 hypothetical protein -
  MX663_RS20170 (MX663_20170) - 3850862..3851659 (+) 798 WP_231573542.1 hypothetical protein -
  MX663_RS20175 (MX663_20175) - 3851662..3852309 (+) 648 WP_247470916.1 ABC transporter ATP-binding protein -
  MX663_RS20180 (MX663_20180) - 3852302..3852862 (+) 561 WP_015714897.1 hypothetical protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45604.09 Da        Isoelectric Point: 5.0624

>NTDB_id=677898 MX663_RS20145 WP_160245473.1 3847274..3848419(+) (rapF) [Bacillus subtilis strain N4]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKENEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AIPYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=677898 MX663_RS20145 WP_160245473.1 3847274..3848419(+) (rapF) [Bacillus subtilis strain N4]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTAATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAATTTCTTCAG
AGGCATGTATGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAAAATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGGAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATACCTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(223-290)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(4-65)

Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3)

(132-208)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

99.475

100

0.995
  rapC Bacillus subtilis subsp. subtilis str. 168

57.368

99.738

0.572