Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MX663_RS02095 Genome accession   NZ_CP095736
Coordinates   385637..386086 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus subtilis strain N4     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 380637..391086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MX663_RS02060 (MX663_02060) yckA 380908..381588 (-) 681 WP_046160037.1 amino acid ABC transporter permease -
  MX663_RS02065 (MX663_02065) yckB 381598..382461 (-) 864 WP_247471197.1 transporter substrate-binding domain-containing protein -
  MX663_RS02070 (MX663_02070) - 382623..382819 (+) 197 Protein_365 hypothetical protein -
  MX663_RS02075 (MX663_02075) - 382855..383175 (+) 321 Protein_366 RDD family protein -
  MX663_RS02080 (MX663_02080) yckD 383256..383588 (+) 333 WP_015252849.1 YckD family protein -
  MX663_RS02085 (MX663_02085) bglC 383742..385175 (+) 1434 WP_247471200.1 6-phospho-beta-glucosidase -
  MX663_RS02090 (MX663_02090) nin/comJ 385212..385610 (-) 399 WP_015715226.1 DNA-entry nuclease inhibitor Regulator
  MX663_RS02095 (MX663_02095) nucA/comI 385637..386086 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  MX663_RS02100 (MX663_02100) tlpC 386254..387975 (-) 1722 WP_213377036.1 methyl-accepting chemotaxis protein -
  MX663_RS02105 (MX663_02105) hxlB 388086..388643 (-) 558 WP_247471201.1 6-phospho-3-hexuloisomerase -
  MX663_RS02110 (MX663_02110) hxlA 388649..389281 (-) 633 WP_003246452.1 3-hexulose-6-phosphate synthase -
  MX663_RS02115 (MX663_02115) hxlR 389514..389876 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=677829 MX663_RS02095 WP_003234608.1 385637..386086(-) (nucA/comI) [Bacillus subtilis strain N4]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=677829 MX663_RS02095 WP_003234608.1 385637..386086(-) (nucA/comI) [Bacillus subtilis strain N4]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGTCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98