Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JLE49_RS01855 Genome accession   NZ_AP022409
Coordinates   407375..408052 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain STW0522-31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 402375..413052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JLE49_RS01840 (STW0522ESC31_03660) chiA 403742..406435 (+) 2694 WP_200922265.1 bifunctional chitinase/lysozyme -
  JLE49_RS01845 (STW0522ESC31_03670) bfd 406604..406798 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  JLE49_RS01850 (STW0522ESC31_03680) bfr 406870..407346 (+) 477 WP_000675504.1 bacterioferritin -
  JLE49_RS01855 (STW0522ESC31_03690) pilD 407375..408052 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  JLE49_RS01860 (STW0522ESC31_03700) gspM 408052..408513 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  JLE49_RS01865 (STW0522ESC31_03710) gspL 408510..409673 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  JLE49_RS01870 (STW0522ESC31_03720) gspK 409688..410671 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  JLE49_RS01875 (STW0522ESC31_03730) gspJ 410664..411251 (-) 588 WP_001402582.1 type II secretion system minor pseudopilin GspJ -
  JLE49_RS01880 (STW0522ESC31_03740) gspI 411244..411621 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  JLE49_RS01885 (STW0522ESC31_03750) gspH 411618..412127 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  JLE49_RS01890 (STW0522ESC31_03760) gspG 412135..412572 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=67737 JLE49_RS01855 WP_000178161.1 407375..408052(-) (pilD) [Escherichia coli strain STW0522-31]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=67737 JLE49_RS01855 WP_000178161.1 407375..408052(-) (pilD) [Escherichia coli strain STW0522-31]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAACTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment